Evaluation of ASPM and TEF Gene Expressions as Potential Biomarkers for Bladder Cancer

Saleh, Amany A.; Gohar, Suzy; Hemida, Aiat Shaban; Elgharbawy, Mohamed; Soliman, Shimaa E.

doi:10.1007/s10528-020-09962-1

Cited by 12 publications

(10 citation statements)

References 33 publications

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“…ASPM gene showed sensitivity of 95.56% and specificity of 86.67%, whilst TEF 91.11% and 71.11%. Furthermore, these two genes have the ability to predict occurrence of metastatic lesions, being significantly over or under expressed in high-grade tumors, and smokers [ 64 ].…”

Section: Resultsmentioning

confidence: 99%

The Role of Novel Bladder Cancer Diagnostic and Surveillance Biomarkers—What Should a Urologist Really Know?

Malinaric

Mantica

Monaco

et al. 2022

IJERPH

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The aim of this review is to analyze and describe the current landscape of bladder cancer diagnostic and surveillance biomarkers. We researched the literature from 2016 to November 2021 to find the most promising new molecules and divided them into seven different subgroups based on their function and location in the cell. Although cystoscopy and cytology are still the gold standard for diagnosis and surveillance when it comes to bladder cancer (BCa), their cost is quite a burden for national health systems worldwide. Currently, the research is focused on finding a biomarker that has high negative predictive value (NPV) and can exclude with a certainty the presence of the tumor, considering missing it could be disastrous for the patient. Every subgroup has its own advantages and disadvantages; for example, protein biomarkers cost less than genomic ones, but on the other hand, they seem to be less precise. We tried to simplify this complicated topic as much as possible in order to make it comprehensible to doctors and urologists that are not as familiar with it, as well as encourage them to actively participate in ongoing research.

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Section: Resultsmentioning

confidence: 99%

The Role of Novel Bladder Cancer Diagnostic and Surveillance Biomarkers—What Should a Urologist Really Know?

Malinaric

Mantica

Monaco

et al. 2022

IJERPH

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“…An improper response to DNA damage is a cancer hallmark and contributes to tumorigenesis and chemo/radiotherapeutic resistance. ASPM overexpression has been observed in various cancers ( Xu et al., 2019 , 2020 ; Zhou et al., 2020 ; Alsiary et al., 2014 ; Bruning-Richardson et al., 2011 ; Saleh et al., 2020 ; Xie et al., 2017 ; Tian and Wang, 2020 ; Timaner and Shaked, 2020 ; Hsu et al., 2019 ; Wang et al., 2013 ; Tang et al., 2019 ; Lin et al., 2008 ; Yuan et al., 2020 ; Wu et al., 2021 ) and thus might serve as a prognostic marker in various contexts. ASPM is a positive regulator of the canonical Wnt/β-catenin signaling ( Major et al., 2008 ; Hsu et al., 2019 ) and thus promotes cancer stemness and progression in prostate cancer ( Pai et al., 2019 ) and hepatocellular carcinoma ( Liao et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

ASPM promotes homologous recombination-mediated DNA repair by safeguarding BRCA1 stability

Wang

et al. 2021

iScience

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Summary DNA double-strand break (DSB) repair by homologous recombination (HR) is essential for ensuring genome stability. Abnormal spindle-like microcephaly-associated ( ASPM ) gene encodes a spindle protein that is commonly implicated in primary microcephaly. We found that ASPM is recruited to sites of DNA damage in a PARP2-dependent manner. ASPM interacts with BRCA1 and its E3 ligase HERC2, preventing HERC2 from accessing to BRCA1 and ensuring BRCA1 stability. Inhibition of ASPM expression promotes HERC2-mediated BRCA1 degradation, compromises HR repair efficiency and chromosome stability, and sensitizes cancer cells to ionizing radiation. Moreover, we observed a synergistic effect between ASPM and PARP inhibition in killing cancer cells. This research has uncovered a novel function for ASPM in facilitating HR-mediated repair of DSBs by ensuring BRCA1 stability. ASPM might constitute a promising target for synthetic lethality-based cancer therapy.

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“…S. aureus DEGs are commonly involved in immune pathways, such as antigen processing, presentation, complement, and coagulation cascades ( Figure 4 A) [ 36 , 37 ]. Furthermore, the unique common DSGs in S. aureus -challenged groups ( TEF , left in Figure 6 A) are related to the progress of cellular death and cancer [ 38 , 39 , 40 ]. These genes provide further broad-spectrum data for anti- S. aureus mastitis strategies.…”

Section: Discussionmentioning

confidence: 99%

Protective Roles of Folic Acid in the Responses of Bovine Mammary Epithelial Cells to Different Virulent Staphylococcus aureus Strains

Tang

Shi

et al. 2021

Biology

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Mastitis caused by Staphylococcus aureus (S. aureus) infection is one of the most difficult diseases to treat in dairy cattle. Exploring the biological progression of S. aureus mastitis via the interaction between host, pathogen, and environment is the key to an effective and sustainable improvement of animal health. Here, two strains of S. aureus and a strain of MRSA (Methicillin-resistant Staphylococcus aureus) isolated from cows with different inflammation phenotypes were used to challenge Mac-T cells and to investigate their effects on the global transcriptome of the cells, then to explore the potential regulatory mechanisms of folic acid on S. aureus mastitis prevention. Differential gene expression or splicing analysis showed that different strains of S. aureus led to distinct transcriptional responses from the host immune system. Folic acid could protect host defense against the challenge of S. aureus and MRSA partially through activating cytoplasmic DNA sensing and tight junction pathway. ZBP1 at the upstream of cytoplasmic DNA sensing pathway was verified and related to anti-pathogen through RNA interference. Further enrichment analysis using these transcriptome data with cattle large-scale genome-wide association study (GWAS) data confirmed that ZBP1 gene is highly associated with bovine somatic cell score (SCS) trait. Our data shed light on the potential effect of FA through regulating key gene and then protect host cells’ defense against S. aureus and MRSA.

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Evaluation of ASPM and TEF Gene Expressions as Potential Biomarkers for Bladder Cancer

Cited by 12 publications

References 33 publications

The Role of Novel Bladder Cancer Diagnostic and Surveillance Biomarkers—What Should a Urologist Really Know?

The Role of Novel Bladder Cancer Diagnostic and Surveillance Biomarkers—What Should a Urologist Really Know?

ASPM promotes homologous recombination-mediated DNA repair by safeguarding BRCA1 stability

Protective Roles of Folic Acid in the Responses of Bovine Mammary Epithelial Cells to Different Virulent Staphylococcus aureus Strains

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