Evaluation of Bone Marrow Mesenchymal Stem Cell Standard Cryopreservation Procedure Efficiency

Preservation of stem cells

“…29 Other investigators used mechanical freezers with Heng and colleagues placing the samples directly into a -80°C freezer 30 and Kotobuki and colleagues 31 placing the samples into a -30°C mechanical freezer followed by a -80°C freezer. Vitrification of the MSCs required plunging of the samples in a cryovial into liquid nitrogen.…”

“…Carvalho and colleagues used culture media supplemented with 10% fetal calf serum (FCS) and 5% dimethyl sulfoxide (DMSO) to freeze MSCs isolated from rat bone marrow. 29 Other investigators used a similar solution composition for the cryopreservation of human MSCs isolated from bone marrow. 30,31 In developing a solution more appropriate for clinical use, Pal, Hanwate and Totey used different parenteral solutions (e.g., saline solution, Plasmalyte A) supplemented with 5% human serum albumin (HSA) and 10% DMSO.…”

“…Some studies have used controlled rate freezing of the samples. 29,32 Carvalho colonies, it is not easy to quantify the fraction of cells that are viable within the colony. Typically the percentage of colonies which are attached is used.…”

Cryopreservation of Rat Bone Marrow Derived Mesenchymal Stem Cells by Two Conventional and Open-Pulled Straw Vitrification Methods

“…There have been several reports to demonstrate the superiority of vitrification to other freezing programs for human ESCs, because it is able to avoid cell injury resulted from ice crystal formation (42,44). Carvalho et al (2008) reported that viability of frozen BMMSCs by slow freezing which was to added 5% DMSO was 94.76% and 90.58% viability before versus after cryopreservation (45). Also, the high survival rate (81.8%) is obtained after cryopreservation of human ESCs by programmed freezing (46).…”

Potential applications of germline cell-derived pluripotent stem cells in organ regeneration

“…42 However, when these cells are cryopreserved, a loss in viability is observed. 43,44 ES cells have hitherto been the most promising cell source for hepatocyte generation. Both mouse and human ES cells have been used for this purpose.…”