Evaluation of capillary electrophoresis assay for CDT on SEBIA's Capillarys System: Intra and inter laboratory precision, reference interval and cut-off

Schellenberg, François; Wielders, Jos P.M.

doi:10.1016/j.cca.2010.07.015

Cited by 45 publications

(29 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Three capillary zone electrophoresis (CE) methods were included: the CEofix CDT procedure [ 11 ] (Suarl é e, Belgium) and the Capillarys [ 12 ] and Minicap methods of Sebia (Lisses, France). Four HPLC methods were included: the candidate reference method [ 8 ], the %CDT by HPLC assay of Bio-Rad (Munich, Germany) with both standard [ 13 ] and fast procedures, and the ClinRep CDT [ 14 ] of Recipe (Munich, Germany).…”

Section: Methodsmentioning

confidence: 99%

Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: III. Performance of native serum and serum spiked with disialotransferrin proves that harmonization of CDT assays is possible

Weykamp

Wielders

Helander

et al. 2013

Clinical Chemistry and Laboratory Medicine

Self Cite

View full text Add to dashboard Cite

Carbohydrate-deficient transferrin (CDT) is a generic term that refers to the transferrin glycoforms whose concentration in blood is temporarily increased by sustained alcohol consumption. Due to high clinical specificity, CDT was proposed as a biomarker of heavy alcohol use and has been available for about 20 years. A number of methods have been developed for CDT measurement based on different analytical techniques and principles and without any harmonization or calibration to a reference method. As a consequence, neither the reference limits nor the cut-off values have been similar across assays, hampering understanding of the diagnostic value of CDT and its routine use. This prompted the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) to initiate a Working Group on Standardization of CDT (WG-CDT). This third publication of the WG-CDT is devoted to testing the commutability of native and disialotransferrin-spiked serum panels as candidate secondary reference materials, in order to prove the harmonization potential of commercial CDT methods. The results showed that assay harmonization reduced the inter-laboratory imprecision in a network of reference laboratories running the HPLC candidate reference method. In the seven commercial methods evaluated in this study, the use of multi-level secondary calibrators of human serum origin significantly reduced the between-method imprecision. Thus, harmonization of CDT measurements by different methods can be achieved using this calibration system, opening the way for a full standardization of commercial methods against a reference method by use of certified reference materials.

show abstract

Section: Methodsmentioning

confidence: 99%

Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: III. Performance of native serum and serum spiked with disialotransferrin proves that harmonization of CDT assays is possible

Weykamp

Wielders

Helander

et al. 2013

Clinical Chemistry and Laboratory Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…BNP was measured in EDTA plasma using a chemiluminescent microparticle immunoassay (CMIA) produced by Abbott for use on the Architect i2000 analyser. The alcohol biomarker, carbohydrate deficient transferrin (CDT), was measured at the Moscow Research and Practical Center on Addictions using the Sebia Capillarys 2 multicapillary analyser [31]. …”

Section: Methodsmentioning

confidence: 99%

Hazardous alcohol consumption is associated with increased levels of B-type natriuretic peptide: evidence from two population-based studies

et al. 2013

View full text Add to dashboard Cite

Russia has very high mortality from cardiovascular disease (CVD), with evidence that heavy drinking may play a role. To throw further light on this association we have studied the association of alcohol with predictors of CVD risk including B-type natriuretic peptide (BNP). Levels of BNP increase primarily in response to abnormal cardiac chamber wall stretch which can occur both as a result of atherosclerosis as well as due to other types of damage to the myocardium. No previous population-based studies have investigated the association with alcohol. We analysed cross-sectional data on drinking behaviour in 993 men aged 25–60 years from the Izhevsk Family Study 2 (IFS2), conducted in the Russian city of Izhevsk in 2008–2009. Relative to non-drinkers, men who drank hazardously had an odds ratio (OR) of being in the top 20 % of the BNP distribution of 4.66 (95 % CI 2.13, 10.19) adjusted for age, obesity, waist–hip ratio, and smoking. Further adjustment for class of hypertension resulted in only slight attenuation of the effect, suggesting that this effect was not secondary to the influence of alcohol on blood pressure. In contrast hazardous drinking was associated with markedly raised ApoA1 and HDL cholesterol levels, but had little impact on levels of ApoB and LDL cholesterol. Similar but less pronounced associations were found in the Belfast (UK) component of the PRIME study conducted in 1991. These findings suggest that the association of heavy drinking with increased risk of cardiovascular disease may be partly due to alcohol-induced non-atherosclerotic damage to the myocardium.

show abstract

“…This method has been already ascertained as highly consistent with the HPLC, recognized and widely accepted as a reference method. To comply with a uniform and convenient manner of data presentation, the results were expressed by percentage of total transferrin concentration as reported elsewhere [17]. …”

Section: Discussionmentioning

confidence: 99%

Serum profile of transferrin isoforms in juvenile idiopathic arthritis: a preliminary study

et al. 2018

View full text Add to dashboard Cite

It is reported that alterations in protein glycosylation are present in adult rheumatic diseases; however, the data related to pediatric rheumatic conditions are very scarce. The aim of this study was to assess the effect of juvenile idiopathic arthritis (JIA) on the serum glycosylation profile of transferrin isoforms. Twenty-five patients with different clinical forms of an active JIA and 22 healthy controls were studied. Serum samples were analyzed by capillary electrophoresis on MINICAP electrophoretic system (Sebia, France) to determine the levels of transferrin isoforms. In patients with JIA, tetrasialotransferrin (median 82.6%; range 68.8–99.5) concentration was lower (P = 0.032), and pentasialotransferrin (median 14%; range 0.5–31.2) was higher (P = 0.020) in comparison to controls (median 84.45; range 79.8–87.4; median 11.55; range 9.7–16.1, respectively). No significant correlations between concentration of transferrin isoforms and disease activity score (JADAS 27) or the degree of disability (VAS and CHAQ) were found. Erythrocyte sedimentation rate and CRP levels correlated positively with disialotransferrin (R = 0.493, P = 0.017; R = 0.850, P < 0.001, respectively) and pentasialotransferrin (R = 0.533, P = 0.006; R = 0.491, P = 0.045, respectively), and negatively with trisialotransferrin (R = − 0.546, P = 0.007; R = − 0.515, P = 0.049, respectively) and tetrasialotransferrin (R = − 0.436, P = 0.029; R = − 0.504, P = 0.039, respectively). This preliminary study shows the shifts in transferrin isoforms profile among patients with JIA. Our data indicate a potential clinical utility of the transferrin isoforms measurement, especially tetrasialotransferrin and pentasialotransferrin. Further prospective studies on larger groups of patients should be conducted to validate the results.

show abstract

Evaluation of capillary electrophoresis assay for CDT on SEBIA's Capillarys System: Intra and inter laboratory precision, reference interval and cut-off

Cited by 45 publications

References 23 publications

Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: III. Performance of native serum and serum spiked with disialotransferrin proves that harmonization of CDT assays is possible

Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: III. Performance of native serum and serum spiked with disialotransferrin proves that harmonization of CDT assays is possible

Hazardous alcohol consumption is associated with increased levels of B-type natriuretic peptide: evidence from two population-based studies

Serum profile of transferrin isoforms in juvenile idiopathic arthritis: a preliminary study

Contact Info

Product

Resources

About