Evaluation of Cell Migration and Cytokines Expression Changes under the Radiofrequency Electromagnetic Field on Wound Healing In Vitro Model

Costantini, Erica; Aielli, Lisa; Serra, Federica; Dominicis, Lorenzo De; Falasca, Katia; Giovanni, Pamela Di; Reale, Marcella

doi:10.3390/ijms23042205

Cited by 16 publications

(8 citation statements)

References 46 publications

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“…whereas NFD at 2% concentration has no significant effect on TGF-ß levels. We believe that NFD at 2% concentration may cause chronic inflammation due to upregulation of inflammatory cells in the inflammatory phase (14) and that staying wound healing in the inflammatory phase rather than advancing to the proliferative phase prevents, rather than promotes, wound healing (24). However, more research is needed to determine the mechanisms involved in the NFD 1% healing capability and why NFD 2% has no such effect, this might be explained in term of over-vasodilation resulting in reduced blood supply and thereby reduced growth factor supplied in the lesion vicinity (25).…”

Section: Discussionmentioning

confidence: 99%

EFFECTS OF FORMULATED TOPICAL NIFEDIPINE OINTMENT ON TGF-β AND ACCELERATION OF FACIAL SKIN WOUND HEALING IN RABBITS

Al-Dabbagh¹,

Al-Mashhadane²,

Al-Sarraj³

2023

MMSL

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The purpose of this study was to evaluate the impact of 1% and 2% topical nifedipine ointments on TGF-β and the acceleration of facial skin wound healing in rabbits. Nifedipine ointments of 1% and 2% were prepared. Fifty healthy male rabbits were involved and distributed into two groups according to the study period: group A (7 days) and group B (14 days). Each group was subdivided into five groups (5 rabbits/group): Group I (Normal): rabbits did not undergo a surgical procedure and did not receive treatment; group II (negative control): rabbits had surgical wounds without treatment; group III (positive control): rabbits had surgical wounds with white petroleum treatment; group IV(nifedipine 1%): rabbits had surgical wounds with nifedipine 1% ointment treatment; and group V(nifedipine 2%): rabbits had surgical wounds with nifedipine 2% ointment treatment. Following euthanasia, blood samples (5 ml) were collected from all animals for TGF-β analysis using an ELISA kit. The TGF-β level in NFD 1% group was significantly higher on the 7 th and14 th day of the study associated with a higher rate of wound closure in comparison to other groups. Conclusions: Nifedipine 1% ointment has beneficial value for improving wound healing, while nifedipine NFD 2% has no such effect.

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Section: Discussionmentioning

confidence: 99%

EFFECTS OF FORMULATED TOPICAL NIFEDIPINE OINTMENT ON TGF-β AND ACCELERATION OF FACIAL SKIN WOUND HEALING IN RABBITS

Al-Dabbagh¹,

Al-Mashhadane²,

Al-Sarraj³

2023

MMSL

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“…Impaired WH is a result of the failure of normal progression through the phases [ 26 ]. In the last few years, an increasing number of reports have evaluated the effects of pharmacologic or physical modulation of tissue repair [ 27 , 28 , 29 , 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Wound-Healing Promotion and Anti-Inflammatory Properties of Carvacrol Prodrugs/Hyaluronic Acid Formulations

et al. 2022

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Background. Wound healing (WH) is a complex process involving several stages, such as hemostasis, inflammation, re-epithelialization, and remodeling. Many factors can impair WH, and different pharmacological approaches were studied to date, but the increase in antibiotic resistance, invasiveness, treatment duration, and high cost, have often hampered the resolution of the wound. In this study, we investigated the possible application of water-soluble carvacrol prodrugs (WSCPs) and hyaluronic acid (HA) and their formulations (WSCPs/HA) to improve WH and regulate the inflammatory response. Materials and methods. Firstly, the cytotoxicity of 0.1, 1 and 10 µg/mL of HA, WSCPs and WSCPs/HA formulations were evaluated on HaCaT cells and THP-1 cell lines. The ability of WSCPs/HA formulations to modulate wound repair was evaluated in an in vitro model of WH, using HaCaT cells at 6, 18, and 24 h. The expression of WH mediators, after wound closure was determined by qRT-PCR. Following, we polarized THP-1 cells in M1/M2-like macrophages and tested the anti-inflammatory properties of WSCPs/HA formulations. After, we tested the in vitro WH model for the effects of conditioned medium (CM) from M1/M2-like cells cultured in the presence of WSCPs/HA. Results. Results showed that WSCPs/HA formulations were able to significantly raise the wound closure rate, compared to the single constituents, promoting a complete wound closure after 18 h for WSCP1/HA (10 µg/mL) and after 24 h for WSCP2/HA (10 µg/mL), modulating the MMPs, TGFβ, and COX-2 gene expression. The effects of CM derived from M1/M2 polarized cells cultured in the presence of WSCPs/HA determined WH regulation, with a better ability of the WSCP2/HA formulation to modulate the time-dependent expression of reparative and inflammatory mediators. Conclusion. Our data underline the possible application of WSCPs/HA formulations as bioactive agents for the regulation of the wound repair process by the modulation of inflammatory and remodeling phases, affecting the activity of immune cells.

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“…The anti-GFP siRNA, with the sense strand sequence of 5′-CAA GCU GAC CCU GAA GUU Ctt-3′, as well as the anti-VEGFA siRNA, with the sense strand sequence of 5′-GCG GAU CAA ACC UCA CCA Att-3′, were previously described in the literature [ 57 ]. For control purposes, a mock siRNA, with the sequence of 5′-UUC UCC GAA CGU GUC ACG Utt-3′, was utilized [ 59 ].…”

Section: Methodsmentioning

confidence: 99%

Polycondensed Peptide-Based Polymers for Targeted Delivery of Anti-Angiogenic siRNA to Treat Endometriosis

Egorova,

Maretina,

Krylova

et al. 2023

IJMS

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Endometriosis (EM) is a prevalent gynecological disease characterized by the abnormal growth of tissue similar to the endometrium outside of the uterus. This condition is accompanied by the development of new blood vessels in endometriotic lesions. While surgical intervention is effective in removing endometriotic lesions, some patients require multiple surgeries. Therefore, finding non-surgical treatments for EM is of great interest. One of the promising approaches is anti-angiogenic therapy using siRNA-therapeutics to target the expression of the VEGFA gene. Peptide-based polymers have shown promise as siRNA delivery systems due to their biocompatibility and ease of modification. We conducted a study to evaluate the effectiveness of the R6p-cRGD peptide carrier as a non-viral vehicle for delivering siRNA to endothelial cells in vitro and endometrial implants in vivo. We investigated the physicochemical properties of the siRNA-complexes, assessed cellular toxicity, and examined the efficiency of GFP and VEGFA genes silencing. Furthermore, we tested the anti-angiogenic effects of these complexes in cellular and animal models. The transfection with siRNA complexes led to a significant increase in VEGFA gene knockdown efficiency and a decrease in the migration of endothelial cells. For the animal model, we induced endometriosis in rats by transplanting endometrial tissue subcutaneously. We evaluated the efficiency of anti-angiogenic therapy for EM in vivo using anti-VEGF siRNA/R6p-RGD complexes. During this assessment, we measured the volume of the implants, analyzed VEGFA gene expression, and conducted CD34 immunohistochemical staining. The results showed a significant decrease in the growth of endometriotic implants and in VEGFA gene expression. Overall, our findings demonstrate the potential of the R6p-cRGD peptide carrier as a delivery system for anti-angiogenic therapy of EM.

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Evaluation of Cell Migration and Cytokines Expression Changes under the Radiofrequency Electromagnetic Field on Wound Healing In Vitro Model

Cited by 16 publications

References 46 publications

EFFECTS OF FORMULATED TOPICAL NIFEDIPINE OINTMENT ON TGF-β AND ACCELERATION OF FACIAL SKIN WOUND HEALING IN RABBITS

EFFECTS OF FORMULATED TOPICAL NIFEDIPINE OINTMENT ON TGF-β AND ACCELERATION OF FACIAL SKIN WOUND HEALING IN RABBITS

Wound-Healing Promotion and Anti-Inflammatory Properties of Carvacrol Prodrugs/Hyaluronic Acid Formulations

Polycondensed Peptide-Based Polymers for Targeted Delivery of Anti-Angiogenic siRNA to Treat Endometriosis

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