Evaluation of Commercial DNA and rRNA Amplification Assays for Assessment of Treatment Outcome in Pulmonary Tuberculosis Patients

Rajalahti, Iiris; Vuorinen, Pauli; Liippo, K.; Mm, Nieminen; Miettinen, A

doi:10.1007/s100960100593

Cited by 7 publications

(6 citation statements)

References 11 publications

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“…More recently, an enhanced MTD has also been approved for smear-negative respiratory specimens of suspect patients [89]. Several studies have evaluated both tests for the detection of M. tuberculosis in clinical samples [90]. Compared with culture and the clinical status, these methods have a high sensitivity and specificity in smear-positive specimens, but lower values are obtained in smear-negative specimens precluding their use as a screen to rule out the disease.…”

Section: Nucleic Acid Amplification Methodsmentioning

confidence: 99%

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Palomino¹

2005

Eur Respir J

147

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Tuberculosis (TB) remains one of the major causes of death from a single infectious agent worldwide. Of great concern for TB control is the emergence of drug resistance. Since there is no cure for some multidrug-resistant strains of Mycobacterium tuberculosis, there is concern that they may spread around the world, stressing the need for additional control measures, such as new diagnostics, better drugs for treatment, and a more effective vaccine.Pulmonary TB can be diagnosed by its symptoms, chest radiography, sputum smear microscopy and by cultivation of M. tuberculosis, which is considered as the gold standard. Recent advances in molecular biology and molecular epidemiology, and a better understanding of the molecular basis of drug resistance in TB, have provided new tools for rapid diagnosis; however, the high cost of most of these techniques, and their requirement for sophisticated equipment and skilled personnel have precluded their implementation on a routine basis, especially in low-income countries.Other nonconventional diagnostic approaches proposed include the search for biochemical markers, detection of immunological response and early detection of M. tuberculosis by methods other than colony counting.In the present article, some of these approaches will be reviewed and the feasibility for their implementation in diagnostic laboratories will be discussed.

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Section: Nucleic Acid Amplification Methodsmentioning

confidence: 99%

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Palomino¹

2005

Eur Respir J

147

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“…The laboratory performance of both commercial and in house TB PCR in the diagnosis of tuberculosis has been extensively evaluated. [1][2][3][4][5][6][7][8][9] However, there are few data on the ''in use'' evaluation of TB PCR as a tool for clinical decision making in the management of tuberculosis.…”

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confidence: 99%

Clinical evaluation of the polymerase chain reaction for the rapid diagnosis of tuberculosis

Cheng

2004

J Clin Pathol

105

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Aims: Use of the polymerase chain reaction for the detection of Mycobacterium tuberculosis (TB PCR) as a basis for making clinical decisions on the initiation of antituberculosis treatment was studied. Methods: A retrospective study involving a cohort of 155 patients being investigated for tuberculosis in an infectious disease consultation service was undertaken. TB PCR was performed on pulmonary and extrapulmonary specimens from these patients. The sensitivity of TB PCR was analysed. Results: Of the 155 patients, 144 fitted the clinical diagnosis of tuberculosis, and 112 of them were culture positive for M tuberculosis. Sixty (58.3%) patients with clinical features suggestive of tuberculosis received antituberculosis treatment based on positive TB PCR alone. Of 224 clinical specimens (138 pulmonary and 86 extrapulmonary) sent for TB PCR, 148 (99 pulmonary and 49 extrapulmonary) were positive in 117 patients. Of the 690 clinical specimens sent for culture, 279 were positive for M tuberculosis in 112 patients. The diagnostic sensitivity of TB PCR was 75.9% (85 of 112) and 81.3% (117 of 144) in patients with culture confirmed and clinically diagnosed tuberculosis, respectively. Using culture as the gold standard, the overall sensitivity of TB PCR was 78.3%, and for pulmonary and extrapulmonary specimens it was 82.3% and 72.0%, respectively. Conclusions: TB PCR is a rapid and reliable test in the diagnosis and management of tuberculosis.

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“…Some studies have found PCR positive results from patients with only a tuberculosis infection or a past history of tuberculosis (Yuen et al 1993, Rajalahti et al 2001. In the present study, there was only one BACTEC ® 12B vial that was PCR positive for MTBC but culture positive for NTM.…”

Section: Discussionmentioning

confidence: 38%

Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens

Gouveia

Eisenach

Vinhas

et al. 2008

Mem. Inst. Oswaldo Cruz

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Evaluation of Commercial DNA and rRNA Amplification Assays for Assessment of Treatment Outcome in Pulmonary Tuberculosis Patients

Cited by 7 publications

References 11 publications

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Clinical evaluation of the polymerase chain reaction for the rapid diagnosis of tuberculosis

Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens

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