Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens

Gouveia, Ana Cláudia Carvalho; Eisenach, Kathleen D.; Vinhas, Solange Alves; Ribeiro, Fabíola Karla; Peres, Renata Lyrio; Dietze, Reynaldo; Hadad, David Jamil; Palaci, Moisés

doi:10.1590/s0074-02762008000400012

Mem. Inst. Oswaldo Cruz

2008

DOI: 10.1590/s0074-02762008000400012

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Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens

Ana Cláudia Carvalho Gouveia

Kathleen D. Eisenach

Solange Alves Vinhas

et al.

Abstract: We evaluated the ability of a PCR assay to identify Mycobacterium tuberculosis complex (MTBC)

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2010

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Cited by 2 publications

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“…Spearman correlation between SOD and (a) CAT, (b) TAS and (c) TNF-Spearman correlation between CAT and both of (a) TAS and (b) TNF-Spearman correlation between TAS and TNF- levelsDISCUSSIONTuberculosis has emerged as the greatest danger to people, threatening the health of millions. PTB is the most common form with insidious onset and illness remains unnoticed for sometime (17). So our study, evaluated the sensitivity of PCR (sputum and serum) for diagnosis of PTB compared to culture which is still considered as the gold standard method for the diagnosis of tuberculosis worldwide.…”

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confidence: 99%

Antioxidants and Tumor Necrosis Factor-α in Patients with Pulmonary Tuberculosis

Hussein¹,

Ghazal²,

Sharkawy³

2010

Journal of High Institute of Public Health

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Background: Tuberculosis (TB), is one of the major common air born infectious bacterial diseases which remains a major worldwide health problem with global mortality. Objective: To evaluate the efficiency of serum samples compared to sputum for the early diagnosis of TB, and to evaluate the levels of superoxide dismutase (SOD), catalase (CAT), total antioxidant status (TAS) and tumor necrosis factor- (TNF-) in patients with pulmonary tuberculosis (PTB). Methods: One hundred patients with clinically suspected PTB and 25 healthy individuals were enrolled in the study. According to the bacteriological results, 78 patients were diagnosed as having PTB infection. These cases were categorized into 69 culture positive cases [sputum and serum PCR positive patients (n=42), sputum PCR positive and serum PCR negative patients (n=16) and sputum and serum PCR negative patients (n=11)] and 9 culture negative and sputum PCR positive cases with radiological lung abnormalities suggestive for PTB. For these 78 cases, erythrocyte SOD, CAT, serum TAS and TNF- were determined. Twenty two patients were culture negative and negative for both sputum and serum PCR. They had no PTB and were not involved in biochemical studies. Results: In all 78 PTB patients, erythrocyte SOD, CAT and serum TAS levels were statistically lower than controls (p<0.05), while TNF- was highly significantly increased (p=0.001). There was a significant direct linear correlation between SOD and CAT and TAS (p<0.0001, r= 0.78; p<0.0001, r= 0.88; p<0.0001, r=0.80 respectively) and a significant reverse linear correlation between TNF- and SOD, CAT and TAS level (p<0.0001, r=-0.55; p<0.0001, r=-0.51; p<0.0001, r=-0.65 respectively). Conclusion: Although the sputum culture is still the gold standard for the diagnosis of patients with PTB, sputum PCR is an efficient method that could be used as an alternative to the culture for the rapid identification of PTB cases. The lower levels of SOD, CAT and TAS may be improved by the antioxidant therapy which may help in better prognosis. Anti TNF-α therapy may help in decreasing the elevated level of TNF-α shown in all PTB patients.

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confidence: 99%

Antioxidants and Tumor Necrosis Factor-α in Patients with Pulmonary Tuberculosis

Hussein¹,

Ghazal²,

Sharkawy³

2010

Journal of High Institute of Public Health

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Molecular identification and prevalence of <i>Mycobacterium tuberculosis</i> complex amongst people living with HIV in Osun state, Nigeria.

Alli¹,

Ogbolu²,

Salawu³

et al. 2010

Af J Clin Exp Micro

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Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens

Abstract: We evaluated the ability of a PCR assay to identify Mycobacterium tuberculosis complex (MTBC)

Cited by 2 publications

References 32 publications

Antioxidants and Tumor Necrosis Factor-α in Patients with Pulmonary Tuberculosis

Antioxidants and Tumor Necrosis Factor-α in Patients with Pulmonary Tuberculosis

Molecular identification and prevalence of <i>Mycobacterium tuberculosis</i> complex amongst people living with HIV in Osun state, Nigeria.

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