Evaluation of Crataeva nurvala extracts as antioxidant, antiproteolytic and cytotoxic against hepato-carcinoma and mouse melanoma cell lines

Hade, Swati N.; Joshi, Prachi; Pilley, Harshda H.; Wadegaonkar, Varsha P.; Wadegaonkar, P. A.

doi:10.7324/japs.2016.60928

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…The qualitative phytochemical screening of methanol extract of stem bark of C. nurvala showed the presence of steroids, triterpenoids and alkaloids. Phytochemical screening of methanol extract of stem bark of C. nurvala revealed the presence of steroid and terpenoids as well as alkaloids, phenolics, flavanoids, tannins and saponins (Hade et al, 2016) which supported our results. Sodipo et al (2000) have reported that alkaloids have been associated with medicinal uses for centuries and one of their common biological properties is their cytotoxicity.…”

Section: Qualitative Phytochemical Analysissupporting

confidence: 90%

Qualitative and Quantitative Analysis (GC-MS) of Methanol Extract of Crataeva nurvala stem bark

Aathira¹,

Bibu²,

Dhanusha³

et al. 2021

J Vet Anim Sci

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Medicinal plants are precious source of bioactive compounds which possess a range of beneficial properties and serve as the major source of medicine for a large proportion of population across the world. Since ancient times, Crataeva nurvala has been used as a vital herb in Ayurvedic system of medicine. In Unani system of medicine the bark of C. nurvala is used as an appetite stimulant and as an agent to decrease the secretion of bile and phlegm. In the present study, the methanol extract of stem bark of C. nurvala was analysed for preliminary phytochemicals and the chemical profiling of the extract was illustrated using gas chromatography mass spectrometry (GC-MS) analysis. The phytochemical analysis revealed that the plant extract contained alkaloids, steroids and triterpenoids. The GC-MS analysis determined the presence of different compounds of biological importance. The identification and characterisation of the phytoconstituents in the extract could pave the way for the discovery of new drugs for various ailments.

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Section: Qualitative Phytochemical Analysissupporting

confidence: 90%

Qualitative and Quantitative Analysis (GC-MS) of Methanol Extract of Crataeva nurvala stem bark

Aathira¹,

Bibu²,

Dhanusha³

et al. 2021

J Vet Anim Sci

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“…The absorbance was measured at 750 nm. Salicylic acid (5 mg/mL) was used as standard [12,16,21,29]. Xanthine oxidase inhibition assay.…”

Section: Determination Of Total Polyphenols Contentmentioning

confidence: 99%

Research on Enzyme Inhibition Potential and Phenolic Compounds From Origanum Vulgare Ssp. Vulgare

Hanganu¹

2020

FARMACIA

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This study assessed for the first time, the enzyme inhibitory properties of the Origanum vulgare ssp. vulgare extract. The main compounds were analysed chromatographically and spectrophotometrically. The enzymatic inhibitory potential was evaluated in vitro, against enzymes that play key roles in different diseases: acetylcholinesterase, urease, tyrosinase, trypsin and xanthine oxidase. A specific phenolic profile was evidenced, with large amounts of rosmarinic acid and luteolin 7-Oglucuronide; two phenolic diterpenes (rosmanol and rosmadial) were identified for the first time in this species. The oregano extract exhibited significant urease, tyrosinase and xanthine oxidase inhibitory effects, in line with the phenolic content. As a result of this study, new potential biological activities have been described for Origanum vulgare extract, which can be further studied and used in the pharmaceutical field. Rezumat În acest studiu se evaluează pentru prima dată potenţialul inhibitor enzimatic al extractului de Origanum vulgare ssp. vulgare (șovârf). Principalii compuşi chimici au fost analizaţi prin metode cromatografice şi spectrofotometrice. Capacitatea inhibitorie a fost evaluată in vitro, asupra unor enzime implicate în anumite afecţiuni: acetilcolinesteraza, ureaza, tirozinaza, tripsina şi xantin-oxidaza. În ceea ce priveşte profilul fenolic al extractului, s-au pus în evidenţă cantităţi mari de acid rozmarinic şi luteolin-7-O-glucuronidă; două diterpene fenolice (rosmanol şi rosmadial) au fost identificate pentru prima dată în această specie. Extractul de șovârf a inhibat semnificativ ureaza, tirozinaza şi xantin-oxidaza, în concordanţă cu conţinutul mare de compuşi polifenolici. Prin acest studiu s-au semnalat noi activităţi biologice potenţiale ale extractului de Origanum vulgare, care deschid noi perspective de interes ştiinţific în domeniul farmaceutic.

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“…Previously anticancer, antimicrobial, antihypertension, analgesic, antiparasitic, antidiabetic and antioxidant activities of C. adansonii have been reported (Zingue et al, 2016;Adjagba et al, 2017). Cytotoxic studies have been carried out in various genera of the family Capparaceae including C. nurvala (Sinha et al, 2013;Ali et al, 2014;Hade et al, 2016;Parvin et al, 2011) and C. nurvala (Da Silva Ferreira et al, 2013).…”

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confidence: 99%

Fluorescence Analysis, Extractive Values and Cytotoxic Screening of Crataeva adansonii DC Leaf and Bark through Brine Shrimp Larvae Assay

Bibi¹,

Din²

2020

SJA

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C rataeva adansonii is a moderate-sized deciduous tree belonging to family Capparaceae (Ryan and Ray, 2004). It is commonly known as Varun, temple plant and sacred garlic pear. It grows well on sandy loam in arid areas. In Pakistan, it is found on the Sub-Himalayan track. Its leaves are trifoliate compound (Figure1; Hoang, 2013). The fruit is globose in shape having a 4-5cm diameter. The flowers are showy Abstract | Crataeva adansonii is a medicinal plant and many folklore uses of this plant are well known to us. Pharmacognostic studies like finding extractive values and nutritional values play a vital role in the determination of adulterated or exhausted drugs. It lays down parameters for authentication and standardization of drugs. The cytotoxic study helps in the evaluation of a drug for its toxicity, which is determined by the prevention of growth and replication. Such drugs can be used to treat cancer, multiple sclerosis and rheumatoid arthritis. During the present study ethanolic extracts of leaf and bark parts of C. adansonii were evaluated through cytotoxic activity. Brine shrimp larvae assay was conducted for this purpose. Extractive values of leaf and bark parts were estimated using n-hexane, chloroform, ethanol, methanol, water, and ether as a solvent. 10g of plant powder was dissolved in 200 ml solvent during various extract preparations Fluorescence study was conducted after treating the powder drug with different reagents including ethanol, methanol, acetic acid, n-hexane, HCl, nitric acid, carrageenan, glacial acetic acid, diethyl ether and picric acid and change in coloration was observed. Distinct fluorescence was exhibited by the drug under the normal daylight and UV-radiations of short wavelength (256nm) and long wavelength (365nm). Both the leaf and bark parts showed maximum extractive values in water (23% and 15% respectively) followed by methanol (15% and 6% respectively. The leaf part showed greater extractive values as compared to the bark. The cytotoxic study revealed that the ethanolic extract crude drugs of each part dose-dependently exhibited cytotoxic activity. At higher concentration (100-1000 µg/ml) the leaf and bark ethanolic extracts showed cytotoxicity and at lower concentration (10 µg/ml) very low cytotoxic effect was observed (Figure 4). The LC 50 values for crude leaf extract were found to be 5.34 and for that of stem bark extract, it was 7.44 ug/ml. This study manifests that the selected plant can be helpful in the preparation of novel pharmaceuticals. The pharmacognostic evaluation of this important medicinal drug will be supportive in the detection of adulteration and drug quality control.

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Evaluation of Crataeva nurvala extracts as antioxidant, antiproteolytic and cytotoxic against hepato-carcinoma and mouse melanoma cell lines

Cited by 5 publications

References 11 publications

Qualitative and Quantitative Analysis (GC-MS) of Methanol Extract of Crataeva nurvala stem bark

Qualitative and Quantitative Analysis (GC-MS) of Methanol Extract of Crataeva nurvala stem bark

Research on Enzyme Inhibition Potential and Phenolic Compounds From Origanum Vulgare Ssp. Vulgare

Fluorescence Analysis, Extractive Values and Cytotoxic Screening of Crataeva adansonii DC Leaf and Bark through Brine Shrimp Larvae Assay

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