Evaluation of Cubilin and Megalin levels in association with vitamin D status in Iraqi patient with DM2

Abstract

Megalin is a large multifunctional endocytic clearance receptor for circulating proteins that has been implicated in vitamin D uptake and delivery to the kidney for activation to 1,25(OH) 2 D .Knockout of the megalin gene in mice usually is lethal but the few survivors were characterized as having severe rickets. The findings suggested that VDBP may be a ligand for megalin. The model proposes that VDBP, carrying vitamin D, is filtered by the glomerulus and reabsorbed by the "cargo" receptor megalin or the megalin/cubilin complex in tubular cells, thereby preventing urinary loss of vitamin D. In addit ion to the classical hypothesis of free vitamin D uptake by diffusion in the proximal convoluted tubules at the basolateral site of the epithelium. The aim of this study was evaluate serum levels of megalin and cubilin in relation with vitamin D . This study included Eighty-four participants of both gender (28 are control and 56 T2DM patients).estimation of serum levels of cubilin and megalin was done using commercial ELISA kit of BT/ China, as well as VD3 measurement.Using of HOMA -IR, DM2 patients were sub divided to insulin resistance (IR) and insulin sensitive (IS) groups.There was significant difference between C and IS patient group (0.222± 0.206), (1.222±0.403) , p-value (0.000) respectively . While there was significant difference between C and IR patient group (0.222± 0.206) , (3.688±1.238) ,p-value (0.000), also there was significant difference between IS and IR patient group (1.222±0.403), (3.688±1.238) , p-value (0.000) respectively. In conclusion, experimental studies and findings in rare disorders of receptor dysfunction have established the important role of megalin and cubilin in association with vitamin D.