Evaluation of Dexamethasone Acetate as a Topical Ophthalmic Formulation

Leibowitz, Howard M.; Kupferman, Allan; Stewart, Robert H.; Kimbrough, Richard L

doi:10.1016/0002-9394(78)90249-0

Cited by 43 publications

(10 citation statements)

References 6 publications

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“…Penetration of the cornea was the same whether its epithelium was intact or abraded, and it was concluded that availability of the drug was limited by the area of free surface between the ointment and the tear film. A similar effect occurs with a suspension of dexamethasone acetate (LEIBOWITZ et al 1978). This hypothesis should be investigated in humans with test substances which are less lipid-soluble and less pharmacologically active.…”

Section: B) Contact With Ocular Surfacementioning

confidence: 76%

“…It appears that general inflammation of the globe as a result of infection or irritation can cause a rise in corneal permeability, e.g., in the case of topical penicillin and lincomycin (NAKATSUE 1971a, b), prednisolone phosphate (KUPFERMAN and LEIBOWITZ 1974c), and dexamethasone phosphate (Cox et al1972;LEIBOWITZ et al 1978;HAMARD et al 1975). In the last paper cited, the rise in aqueous humor concentration could be correlated with a subjective scoring of the degree of in flamm ation.…”

Section: Topical Applicationmentioning

confidence: 97%

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Ocular Pharmacokinetics

Maurice¹,

Mishima²

1984

Handbook of Experimental Pharmacology

224

169

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Section: B) Contact With Ocular Surfacementioning

confidence: 76%

Section: Topical Applicationmentioning

confidence: 97%

Ocular Pharmacokinetics

Maurice¹,

Mishima²

1984

Handbook of Experimental Pharmacology

224

169

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“…Glucocorticoids have metabolic and anti‐inflammatory properties and help protect against stress and shock. Artificial glucocorticoids have been synthesized and used in many veterinary therapeutic drugs for the treatment of inflammatory diseases 3–6. These compounds also increase weight gain by means of water and fat retention, and they have a synergetic effect when combined with compounds like β ‐agonists or anabolic steroids 7–9.…”

mentioning

confidence: 99%

Simultaneous determination of seventeen glucocorticoids residues in milk and eggs by ultra‐performance liquid chromatography/electrospray tandem mass spectrometry

Cui

Shao

Zhao

et al. 2006

Rapid Comm Mass Spectrometry

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A comprehensive analytical method has been developed and validated for the simultaneous determination of seventeen glucocorticoid residues in eggs and milk. The mass spectrometer parameters, the composition of the mobile phase and the sample preparation method were firstly optimized to obtain maximum sensitivity. The samples were deconjugated with beta-glucuronidase/arylsulfatase enzyme and concentrated using an Oasis HLB solid-phase extraction cartridge, followed by cleanup with a dual Sep-pak silica and aminopropyl cartridge. The analytes were quantified by ultra-performance liquid chromatography (using a C18 column)/electrospray ionization tandem mass spectrometry (UPLC/ESI-MS/MS) operating in the negative ion mode. The assay for the 17 glucocorticoids was linear over the range of 1-200 microg/L for milk and egg samples with a high correlation coefficient (>0.99). The limits of quantification (LOQs) for the target analytes were 0.04-1.27 microg/kg for the egg samples and 0.03-0.73 microg/kg for the milk samples. The average extraction recoveries of the glucocorticoids from eggs and milk at two concentration levels (spiked at 0.40 and 2.00 microg/kg) were 65.6-118.7% and 61.5-119.6%, respectively, with relative standard deviations between 1.8-17.0% and 2.4-18.4%, respectively. Because of its high sensitivity, good precision and specificity, the method was found to be suitable for trace analysis of synthetic and natural glucocorticoids in complex biosamples such as eggs and milk.

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“…42 To date, steroid therapy has been the standard antiinflammatory and angiostatic treatment in the cornea. 28,29 Our results suggest that specific molecular or cellular targeting strategies, such as blockade of NF-B or regulation of the activation status of corneal stromal cells, may offer novel approaches in the treatment of inflammatory angiogenesis in the cornea.…”

Section: Discussionmentioning

confidence: 99%

“…28,29 Dexamethasone inhibits NF-B but not AP-1 activity in transfected human corneal fibroblasts. 30 We show that IL-1␤-induced NF-B activation is inhibited by dexamethasone and that a selective NF-B inhibitor diminishes inflammatory corneal angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

Dexamethasone Inhibits Interleukin-1β-Induced Corneal Neovascularization

Nakao

Hata

Matsuda

et al. 2007

The American Journal of Pathology

104

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Dexamethasone, a synthetic corticosteroid, is widely used as a potent anti-inflammatory drug in various diseases including corneal angiogenesis. However, dexamethasone's impact on interleukin (IL)-1␤-dependent inflammatory angiogenesis is unknown. Here, we show that dexamethasone inhibits IL-1␤-induced neovascularization and the expression of the angiogenesis-related factors, vascular endothelial growth factor-A, KC, and prostaglandin E 2 in the mouse cornea 2 days after IL-1␤ implantation. IL-1␤ caused IB-␣ phosphorylation in corneal stromal cells but not in infiltrated CD11b

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Evaluation of Dexamethasone Acetate as a Topical Ophthalmic Formulation

Cited by 43 publications

References 6 publications

Ocular Pharmacokinetics

Ocular Pharmacokinetics

Simultaneous determination of seventeen glucocorticoids residues in milk and eggs by ultra‐performance liquid chromatography/electrospray tandem mass spectrometry

Dexamethasone Inhibits Interleukin-1β-Induced Corneal Neovascularization

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