Evaluation of DXS9902, DXS7132, DXS6809, DXS7133, and DXS7423 in humans and chimpanzees: sequence variation, repeat structure, and nomenclature

Abstract

Sequencing data obtained in this study provide information on the short tandem repeat allele structures of DXS9902, DXS7132, DXS6809, DXS7133, and DXS7423. Data were obtained from the three human major population groups, namely Africans, Caucasians, and Asians as well as from chimpanzees (Pan troglodytes). DXS7133 was found to be the most stable locus and DXS6809 seemed to have evolved from a simple array of CTAT units but currently reveals a highly complex and compound structure within and between humans and chimpanzees. DXS9902 results support a TAGA allele nomenclature, which increases in one repeat unit previously reported allele distributions at this locus. For DXS7132, human/chimpanzee comparisons performed in this study provided important evidence that the CTAT allele structure should be considered for allele nomenclature purposes. Also, possible population-specific intermediate type alleles (with Native American origin) were detected at this locus that could be useful for ethnic group differentiation. DXS7423 results revealed two different sequence structures and one of these structures seems to be restricted to a single allele class in just one population group (Africans).

Diversity study of 12 X‐chromosomal STR loci in Hui ethnic from China

“…. Hence, X‐STRs have been the focus in many researches of both population genetics and forensic sciences .…”

Genetic insight into Nigerian population groups using an X-chromosome decaplex system

“…Thermocycling conditions used for the DNA amplification……….……42 Table 6. Primer sequences used for sequencing according to (Gomes et al, 2009a, Gomes et al, 2009c Table 7. Thermocycling conditions used for the purification step………….....…46 Table 8.…”

“…Table 6. Primer sequences used for sequencing according to (Gomes et al, 2009a, Gomes et al, 2009c Final sequenced products were purified with 5% Sephadex using in-house filtration columns, following the protocol:…”

“…In addition, allele 31.1 presented a T->C transition mutation at the 3 rd base nucleotide at the tenth-base nucleotide stretch attatctatc (that is not considered for allele nomenclature by the described structure) and does not interfere with fragment size. In the study by Gomes and collaborators (Gomes et al, 2009a)…”

False paternity with one or two mismatches using commercial STR kits

“…The X-chromosome analysis was carried out by using Genephile X-Plex Human X Chromosome STR mapping set (GenePhile Bioscience Co., Ltd., Taipei, Taiwan) that selectively amplified the following STR markers; DXS8378, DXS9898, DSX8377, HPRTB, GATA172D05, DXS7423, DXS6809, DXS7132, DXS101, DXS6789, DXS9902, DXS6807 and DXS7424. These STR loci have been evaluated for forensic purpose by many different groups [13][14][15][16] . PCR amplification was carried out by using Veriti thermal cycler (Applied Biosystems).…”