Evaluation of Expression of Human Epidermal Growth Factor Receptor 2 (HER2) in Gastric and Gastroesophageal Junction Adenocarcinoma Using IHC and Dual-ISH

Domínguez, Carolina; Rosa, Marilin; George, T.; Pimiento, José M.; Lauwers, Gregory Y.; Coppola, Domenico

doi:10.21873/anticanres.12231

Cited by 6 publications

(2 citation statements)

References 14 publications

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“…The detection results are affected by many factors, as well as the quality control of the laboratory and the interpretation of staining results. Some studies indicated that the expression rate of HER2 protein was significantly different from 6% to 34% [ 18 – 21 ]. In our research, HER2 protein was detected by combining the positive intensity and the positive area, which was divided into extensive staining, partial staining, and focal staining.…”

Section: Discussionmentioning

confidence: 99%

Significance of Detection of the HER2 Gene and PD-1/PD-L1 in Gastric Cancer

Yun

Wang

Jiang

et al. 2020

Journal of Oncology

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Objective. To explore the relationship between the HER2 gene and PD-1/PD-L1 in gastric cancer and its significance. Methods. Immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) were used to detect HER2 protein expression, HER2 gene amplification, and PD-1/PD-L1 expression in 78 cases of gastric cancer. Results. The expression rate of HER2 protein was 43.6% (34/78), of which 19.4% (14/78) were HER2 3+, 14.1% (11/78) were HER2 2+, and 11.5% (9/78) were HER2 1+. The results showed that 19.2% (15/78) of samples had HER2 gene amplification, 3.8% (3/78) of samples had a HER2/CEP17 ratio <2.0, and 19.2% (15/78) of samples had HER2 gene amplificationf and HER2 copy/cell ≥6.0, as detected by FISH. The positive rate of PD-L1 was 38.5% (30/78) in gastric cancer cells and 50.0% (39/78) in interstitial lymphocytes. The expression of the HER2 gene, PD-L1, and PD-1 in gastric cancer was correlated with the stage and lymph node metastasis of gastric cancer ( P < 0.05 ). Conclusions. The combined detection of the HER2 gene and PD-1/PD-L1 in gastric cancer provides an important reference index for the prognosis of gastric cancer and the benefit of targeted antitumor drugs.

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Section: Discussionmentioning

confidence: 99%

Significance of Detection of the HER2 Gene and PD-1/PD-L1 in Gastric Cancer

Yun

Wang

Jiang

et al. 2020

Journal of Oncology

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“…Immunohistochemical analysis was performed as previously described ( Dominguez et al, 2018 ). The tumor or normal tissues were collected and perfused with 10% formaldehyde solution for 24 h at 4°C, followed by embedment in paraffin.…”

Section: Methodsmentioning

confidence: 99%

Artesunate Suppresses the Proliferation and Development of Estrogen Receptor-α-Positive Endometrial Cancer in HAND2-Dependent Pathway

Yin

Liu

Qin

et al. 2021

Front. Cell Dev. Biol.

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Endometrial cancer (EC) is a common leading cause of cancer-related death in women, which is associated with the increased level of estrogen in the body. Artesunate (ART), an active compound derived from Artemisia annua L., exerted antitumor properties in several cancer types. However, the role of artesunate and the molecular basis on EC remains unclear. Here, we aimed to explore the effects and mechanisms of artesunate. Our results identified that estrogen receptor-α (ER-α) was a key factor for the type I EC (ER-α-positive), which might suppress the downstream LKB1/AMPK/mTOR pathway. Besides, we found ART significantly inhibited tumor proliferation in a dose-dependent manner. Mechanistic studies identified that ART led to tumor cell apoptosis and cell cycle arrest by downregulating the ER-α expression and activating the LKB1/AMPK/mTOR pathway. In addition, we found ART could increase the expression of heart and neural crest derivatives expressed 2 (HAND2) in the ER-α-positive EC cells, which could interact with ER-α. Through the gain-and loss-function experiments, we showed that over expression of HAND2 repressed the proliferation and migration of ER-α-positive EC cells via inhibition of ER-α expression. HAND2 knockdown increased ER-α expression and alleviated the antitumor effect of ART in vitro and in vivo. Overall, our study first showed that ART could be an effective antitumor agent through modulating ER-α-mediated LKB1/AMPK/mTOR pathway in the HAND2 dependent manner. Our findings provide an effective therapeutic agent for ER-α-positive EC treatment.

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The Importance of Human Tissue Bioresources in Advancing Biomedical Research

Diffalha

Sexton

Watson

et al. 2019

Biopreservation and Biobanking

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Medical research advances enabling the realization of precision medicine have relied heavily on the biospecimens provided by bioresources to identify the targets and biomarkers that are the focus of the new generation of more effective molecular-based therapies for specific subtypes of diseases. Through the biospecimens they have distributed, bioresources have permitted subtypes of cancers to be identified and molecular features of these subtypes to be effectively targeted. A prototype example is the human epidermal growth factor receptor type 2 (HER2), which currently is targeted in breast and gastric cancers. In the future, the use of biospecimens from bioresources will continue to increase the understanding of the molecular actions of drugs and how drugs may be more or less active in subpopulations of patients. Although the biospecimen inventories of the initial forms of bioresources may not have always been optimally planned and, therefore, utilized in supporting biomedical research, bioresources are evolving and overall, bioresource inventories and increasingly their prospective collection capabilities will continue to be a critical component of the research infrastructure.

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Evaluation of Expression of Human Epidermal Growth Factor Receptor 2 (HER2) in Gastric and Gastroesophageal Junction Adenocarcinoma Using IHC and Dual-ISH

Cited by 6 publications

References 14 publications

Significance of Detection of the HER2 Gene and PD-1/PD-L1 in Gastric Cancer

Significance of Detection of the HER2 Gene and PD-1/PD-L1 in Gastric Cancer

Artesunate Suppresses the Proliferation and Development of Estrogen Receptor-α-Positive Endometrial Cancer in HAND2-Dependent Pathway

The Importance of Human Tissue Bioresources in Advancing Biomedical Research

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