Evaluation of Extractive Value and In-Vitro antimicrobial potential of Curcuma longa using disk diffusion method

Abstract

The efficiency of turmeric fractions, such as petroleum ether, chloroform, benzene, methanol and aqueous were evaluated for their inhibitory effect on clinical and standard strains of pathogenic bacteria and fungus. The methanolic fraction of Curcuma longa rhizome had high potential to inhibit some pathogenic bacteria i.e. S. aureus to a greater degree than other fractions of Curcuma longa. In our study the results show that the different fractions (petroleum ether, methanol etc.) of Curcuma longa rhizome were more effective antimicrobial agents than the crude extract of Curcuma longa. Methanolic extract of curcuma longa was evaluated against gram positive and gram-negative bacteria. The bacteria isolates include Bacillus subtilis and Escherichia coli. Antimicrobial activities were estimated by Well diffusion method and antifungal activity was evaluated against Candida Albicans and Aspergillus Niger. The results were pointed as the methanol extract was more effective on both bacteria and fungus then water also similar effective on both bacteria and fungus but against Aspergillus Nigerit has outstand results. 
 Keywords: Curcuma longa, Phytochemical, Antimicrobial, Free radical, Scavenging Potential