Evaluation of Fungal Laccase Immobilized on Natural Nanostructured Bacterial Cellulose

Chen, Lin; Zou, Min; Hong, Feng

doi:10.3389/fmicb.2015.01245

Cited by 60 publications

(56 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The values of enzyme activity obtained for native laccase are comparable to some reported results in literature being, as predicted, more pronounced on swelled samples [31]. The catalyst is therefore more accessible to react with the ABTS [31][32][33] as well as with other substrates. [10] used BC as support for laccase immobilization by adsorption-crosslinking and obtained a maximum retained activity of 69%.…”

Section: Immobilization Of Native and Pegylated Laccase On Swelled Basupporting

confidence: 90%

“…The bacterial cellulose was produced using HS medium composed of: 20 g/L glucose, 5 g/L yeast extract, and 3 g/L peptone [10,11]. Commercial BC gel from scoby was added to the culture medium in a liquor ratio of 1:5 (v/w).…”

Section: Production Of Bacterial Cellulosementioning

confidence: 99%

“…It must be noteworthy that the activity of laccase decreases considerably after PEGylation. [10] used BC as support for laccase immobilization by adsorption-crosslinking and obtained a maximum retained activity of 69%. Table 5 shows the activity of enzymes immobilized on swelled and non swelled BC samples.…”

Section: Immobilization Of Native and Pegylated Laccase On Swelled Bamentioning

confidence: 99%

“…The nanostructured network of BC is expected to easily entrap enzyme molecules and, due to its large surface area, high mechanical strength, and porosity to promote high protein loading [3][4][5][6]. The three-dimensional porous structure of BC provides also high accessibility onto the active site through low diffusion resistance of the substrate [10][11][12]. BC structures are considered excellent supports for enzyme immobilization since they can enhance enzyme stability against pH and temperature changes [11].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ultrasound‐assisted swelling of bacterial cellulose

Song

Loureiro

et al. 2017

Engineering in Life Sciences

View full text Add to dashboard Cite

Bacterial cellulose (BC) was obtained by static cultivation using commercial BC gel from scoby. BC membranes (oven dried and freeze‐dried) were swelled with 8% NaOH, in the absence and in the presence of ultrasound (US), for 30, 60, and 90 min. The influence of swelling conditions on both physico‐chemical properties and molecules entrapment was evaluated. Considering the highest levels of entrapment, an optimum swelling procedure was established: 8% NaOH for 30 min at room temperature in the presence of US. Native and PEGylated laccase from Myceliophthora thermophila was immobilized on BC membranes and a different catalytic behaviour was observed after immobilization. Native laccase presented activity values similar to published reports (5–7 U/gBC) after immobilization whereas PEGylated enzymes showed much lower activity (1–2 U/gBC). BC swelled membranes are presented herein as a potential support for the preparation of immobilized enzymes for industrial applications, like phenolics polymerization.

show abstract

Section: Immobilization Of Native and Pegylated Laccase On Swelled Basupporting

confidence: 90%

Section: Production Of Bacterial Cellulosementioning

confidence: 99%

Section: Immobilization Of Native and Pegylated Laccase On Swelled Bamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ultrasound‐assisted swelling of bacterial cellulose

Song

Loureiro

et al. 2017

Engineering in Life Sciences

View full text Add to dashboard Cite

show abstract

“…Thus, additional external and internal diffusion steps for the educts and products upstream and downstream from the actual reaction event determine the overall (heterogeneous) kinetics, as also known from heterogeneous catalysis. In other words, the kinetics of the immobilized laccase depend on the diffusion coefficient of the substrate and on the capability of the ABTS molecules to diffuse through the highly porous loofa network …”

Section: Methodsmentioning

confidence: 99%

Enzymatic decolourization of water using loofa sponge as cellular carrier: Immobilization and dye degradation performance

et al. 2018

View full text Add to dashboard Cite

In recent years, strong efforts have been made to develop sustainable biocatalytic decolourization processes for dye‐polluted water. In particular, dye‐oxidizing laccase enzymes immobilized on suitable carriers are promising candidates, and can be reused as long as the activity is sufficiently high. In this work, we propose, for the first time, a new methodology to immobilize laccase from Trametes hirsute on a naturally‐grown and decomposable cellular loofa sponge carrier and assess the capability to degrade dye‐polluted water. High immobilization activity is achieved and about 70 % residual activity remains after 8 cycles. Additionally, we determined homogenous and heterogeneous kinetic parameters for free and immobilized enzymes. The results reveal a four times higher Michaelis‐Menten constant of the laccase immobilized on the loofa due to mass transfer and mixing limitations in a packed bed bio‐reactor. The response surface methodology was applied to identify favourable operating conditions. In particular, dye concentration and treatment time determine the decolourization performance, while mixing velocity has only a minor effect. Eventually, the results demonstrated a remarkable dye removal capability with shorter treatment time compared to the previous studies on immobilized laccase reported in the literature.

show abstract