Evaluation of Gellan Gum Film Containing Virgin Coconut Oil for Transparent Dressing Materials

Abstract

We examined the potential of virgin coconut oil (VCO) incorporated in gellan gum (GG) films as a dressing material. Pure GG film is extremely brittle and inclusion of 0.3% (w/w) VCO in the GG film (GG-VCO3) improved the toughness (T≈0.67±0.33 J g−1) of the composite films. Swelling properties and water vapor transmission rates of GG-VCO composite films decreased, whereas thermal behavior values increased upon the addition of higher concentrations of VCO. Cell studies exhibit that the VCO is noncytotoxic to human skin fibroblast cells (CRL2522) with limited cell growth observed on GG-VCO3 films at 1,650 cells/well after incubation for 72 h which could be due to hydrophobic influence of the material surface. The qualitative andin vitroquantitative antibacterial results revealed that VCO does not possess strong bacterial resistance against all four tested bacteria, that is, two Gram-positive (Staphylococcus aureus and Staphylococcus epidermidis) and two Gram-negative bacteria (Pseudomonas aeruginosa and Proteus mirabilis).

Gellan gum/clay hydrogels for tissue engineering application: Mechanical, thermal behavior, cell viability, and antibacterial properties

“…13 The physical and mechanical properties, including hardness and roughness of hydrogel containing clays could be another reason cells were dying. 17,42 In addition, the solubility of clays in biopolymer solutions also plays an important role which may influence the growth of cell as reported by a previous study. 30 They demonstrated that the reduction of weight percentage of polyethylene glycol (PEG) in polypeptide-PEG exhibits better cell adhesion.…”

“…It is also used as a scaffold material for tissue engineering application. 14,15 GG has been reported to have good biocompatibility on mouse fibroblast cells (L929), 16 human skin fibroblast cells (CRL2522), 17 and human fetal osteoblasts (hFOBs 1.19). 18 In general, hydrogel can be classified as a three dimensional polymer network which has received more attention in past decade due to its ability to absorb and retain high amount of water.…”

Gellan Gum Hydrogels Filled Edible Oil Microemulsion for Biomedical Materials: Phase Diagram, Mechanical Behavior, and In Vivo Studies

“…GG has a broad band at 3476 cm −1 , which is a typical region for stretching of the O-H group (3000–3500 cm −1 ) [ 27 ]. A new distinguishing peak observed at 1638–1675 cm −1 in GVCO hydrogels correlates to the shifting of -C-H (-CH 2 ) bending scissoring of VCO and C=C due to the appearance of phenolic content and stretching of that aromatic ring [ 28 ]. The stretching alkyl signal in VCO microemulsion at 2943 cm −1 shifted to 2930–2920 cm −1 in GVCO hydrogels confirming the hydrogen bonding interaction in GVCO blends [ 28 ].…”

“…This facilitates the tight packaging of gellan gum chains, resulting in fragile hydrogels. However, the addition of VCO microemulsion that contains lauric acid (C12 ≈ 78%) and a hydroxyl group is responsible for promoting the formation of hydrogen bonds between gellan gum and VCO microemulsion [ 28 ]. This bonding replaces the hydrogen bonds between gellan gum chains and thus decreases the intermolecular bonds along polymer chains and improves strain-at-break [ 6 ] of the hydrogels regarding the content of VCO microemulsion.…”

Fabrication and Characterization of Antibacterial Titanium Dioxide Nanorods Incorporating Gellan Gum Films

“…GG is an anionic straight chain biopolymer containing β-D-glucose, α-L-rhamnose and β-D-glucuronate residues 1 and usefulness industrially due to its unique structure and physical properties. Previously, GG has been approached in wound healing study 2,3 as well as candidate material for tissue engineering application 4 and hold a vital role in pharmaceutical. Since 1992, this materials were approved to be utilized in food industry by the US FDA (United States Food and Drug Administration) and the EU (European Union) regulation classified as E 415 5 .…”