Evaluation of Gengigel ®  Application in the  Management of Furcation with Coronally  Advanced Flap through Surgical Re-Entry-  A Split Mouth Clinical Study

Gupta, Sandeep; Kediege, Suresh D; Gupta, Akanksha; Jain, Kishor S.

doi:10.7860/jcdr/2017/21938.9169

Cited by 10 publications

(8 citation statements)

References 30 publications

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“…Gengigel ® contains 0.2% hyaluronic acid (HA) gel that provides stability and elasticity to tissues and delays penetration of viruses and bacteria. 66 This experiment confirmed that a mixture of HA and sEV in an appropriate ratio can still maintain the injectability and release 80% of sEV slowly within one week without affecting the biological functions of sEV. Therefore, HA is a convenient, safe, and effective scaffold material that is suitable for auxiliary sEV in clinical applications.…”

Section: Discussionsupporting

confidence: 65%

Lipopolysaccharide-Preconditioned Dental Follicle Stem Cells Derived Small Extracellular Vesicles Treating Periodontitis via Reactive Oxygen Species/Mitogen-Activated Protein Kinase Signaling-Mediated Antioxidant Effect

Huang¹,

Liu²,

Liu³

et al. 2022

IJN

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Lipopolysaccharide (LPS) pretreatment can enhance the therapeutic effect of dental follicle stem cells-derived small extracellular vesicles (DFC-sEV) for periodontitis, and this study aimed to investigate the underlying mechanisms and clinical application Of LPS-preconditioned DFC-sEV in periodontitis. Methods:The protein spectrum of DFC-sEV before and after LPS pretreatment was determined by liquid chromatography-tandem mass spectrometry and bioinformatic analysis. Their effects on inflammatory periodontal ligament stem cells (PDLSCs) and macrophages were investigated for cell proliferation, migration, type 2 macrophage (M2) polarization, and intracellular reactive oxygen species (ROS) levels separately. In addition, the regulation of ROS/Jun amino-terminal kinases (JNK) and ROS/extracellular signalrelated kinases (ERK) signaling by LPS-preconditioned DFC-sEV was also studied to reveal the antioxidant mechanism. In vivo, two kinds of DFC-sEV loaded with 0.2% hyaluronic acid (HA) gel were applied for canine periodontitis to evaluate the therapeutic potential. Results:The proteomic analysis showed that thirty-eight proteins were differentially expressed in LPS-preconditioned DFC-sEV, and interestingly, the highly expressed proteins were mainly involved in antioxidant and enzyme-regulating activities. In addition to promoting PDLSCs and macrophage proliferation, LPS-preconditioned DFC-sEV inhibited intracellular ROS as an antioxidant. It reduced the RANKL/OPG ratio of PDLSCs by inhibiting ROS/JNK signaling under inflammatory conditions and promoted macrophages to polarize toward the M2 phenotype via ROS/ERK signaling. Furthermore, LPS-preconditioned DFC-sEV loaded with the HA injectable system could sustainably release sEV and enhance the therapeutic efficacy for periodontitis in canines. Conclusion: LPS-preconditioned DFC-sEV could be effectively used as an auxiliary method for periodontitis treatment via antioxidant effects in a subgingival environment, and loading it with HA is feasible and effective for clinical applications.

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Section: Discussionsupporting

confidence: 65%

Lipopolysaccharide-Preconditioned Dental Follicle Stem Cells Derived Small Extracellular Vesicles Treating Periodontitis via Reactive Oxygen Species/Mitogen-Activated Protein Kinase Signaling-Mediated Antioxidant Effect

Huang¹,

Liu²,

Liu³

et al. 2022

IJN

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“…Additionally, rubbing for 1–2 min to achieve the proper effect is difficult in practice. 10 Therefore, challenges remain to be addressed in the field of HA delivery.…”

Section: Introductionmentioning

confidence: 99%

Orodispersible hyaluronic acid film delivery for oral wound healing in rats

Lee

Nam

et al. 2022

Journal of Dental Sciences

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“…This barrier function of hyaluronic acid is very important in the wound healing process, and it is a very promising material for improving the results of periodontitis treatment. [42][43][44][45][46][47] Results our study show that Gengigel Hyaluronic acid preparation used in combination with collapan is a good adjunct to conventional periodontitis therapy. Study demonstrates the higher efficiency and osteoconductive properties of CollapanL for the repair of intrabone defects.…”

Section: Citation: Hakobyan G (2022) Et Al Correlation Of Level Bone ...mentioning

confidence: 63%

Correlation of level bone metabolism biomarker Osteocalcin in serum with periodontal treatment outcome

Hakobyan

Yessayan

Vardevanyan³

et al. 2022

JBSMFS

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Objectives: Assess the diagnostic value of Bone Metabolism Biomarker Osteocalcin for Monitoring Treatment Chronic moderate Periodontitis. Materials and Methods: 84 patients with chronic moderate periodontitis were participated in this study who received periodontal therapy. All patients underwent a thorough clinical and laborator examination including a determination of the serum osteocalcin content, at the beginning of the study and 1, 3 months after therapy. Results: Healing period occurred without complications, and with minimal postoperative discomfort. The mean BOP before treatment was 2.5 ± 0.31, after 1 month treatment the mean BOP 1.7 ± 0.1, after 3 months treatment the mean BOP 1.3 ± 0.12(p>0.05). The probing depth according to the mean value (PD) was 5.27 ± 0.77 mm at the beginning of the study and, respectively, 4.35 ± 0.73 mm 1 month after therapy and 2.82 ± 0.43 (p> 0.05 ) 3 months after therapy. The mean value of clinical attachment level (CAL) at baseline in the study group was 5.84± 0.79 mm, 1 month after therapy it was was 4.92 ±0.71 mm. and 3 month after therapy it was3,4 ±0,7. Therefore, in periodontitis patients; there was a statistically significant decrease in mean CAL post-operatively (at P < 0.001).The mean value of osteocalcin in serum at baseline was 10.58 ng/ml, 1 month after periodontal therapy it was 14.23 ng/ml, 3 month after periodontal therapy it was was 17.74 ng/ml. As a result, there is a statistically significant increase in mean osteocalcin postoperatively (at P < 0.001).The study results showed that elevated serum osteocalcin concentration in patients with chronic periodontitis after periodontal therapy correlates with PPD and CAL, suggesting that its role could be used as a diagnostic test for patient and to complement other diagnostic tests. Conclusions: The results of study showed that a combination treatment with the use of bone graft materials, hyaluronic acid (Gengigel®) and magnetic laser therapy is an effective therapy for the treatment of periodontal disease.

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Evaluation of Gengigel ® Application in the Management of Furcation with Coronally Advanced Flap through Surgical Re-Entry- A Split Mouth Clinical Study

Cited by 10 publications

References 30 publications

Lipopolysaccharide-Preconditioned Dental Follicle Stem Cells Derived Small Extracellular Vesicles Treating Periodontitis via Reactive Oxygen Species/Mitogen-Activated Protein Kinase Signaling-Mediated Antioxidant Effect

Lipopolysaccharide-Preconditioned Dental Follicle Stem Cells Derived Small Extracellular Vesicles Treating Periodontitis via Reactive Oxygen Species/Mitogen-Activated Protein Kinase Signaling-Mediated Antioxidant Effect

Orodispersible hyaluronic acid film delivery for oral wound healing in rats

Correlation of level bone metabolism biomarker Osteocalcin in serum with periodontal treatment outcome

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