Evaluation of hair roots for detection of methamphetamine and 3,4-methylenedioxymethamphetamine abuse by use of an HPLC–chemiluminescence method

Abstract

We describe the use of hair roots as a matrix for detection of methamphetamine (MP) and 3,4-methylenedioxymethamphetamine (MDMA) abuse. The concentration of drugs was determined in rat hair roots, hair shafts, and plasma after a single administration of MP or MDMA, by use of an HPLC-peroxyoxalate chemiluminescence (PO-CL) method involving column switching. Plasma and hair roots and shafts were collected from male Wistar rats before and after administration of MP (10 mg kg(-1), i.p.). In addition, the roots and shafts of pigmented and non-pigmented hair of male Lister hooded rats were collected after administration of MDMA (10 mg kg(-1), i.p.). The concentrations of MP and MDMA in plasma and hair were determined by use of the HPLC-PO-CL method, with satisfactory sensitivity and reproducibility. The concentration of MP in hair roots 1-14 days after administration ranged from 0.038 to 0.115 ng mg(-1) (n = 3). By use of the HPLC-PO-CL method, MP could be detected in hair roots for longer (up to 14 days) than it could be detected in conventional biological specimens, for example plasma (~1 day), and MDMA was detected in hair roots from 1 to 10 days after administration. The AUC(1-10) (ng day mg(-1)) for MDMA in roots of non-pigmented and pigmented hair was comparable (4.93 ± 2.09 vs. 6.67 ± 1.28, n = 3), whereas AUC(1-14) for hair shafts differed significantly (1.86 ± 0.93 vs. 4.58 ± 0.63, P < 0.05, n = 3). The window for detecting MP (or MDMA) in hair roots under our conditions was 1-14 (or 1-10) days.

Determination of Trace Amounts of Methamphetamine in Biological Samples by Hollow Fiber Liquid-phase Microextraction Followed by High Performance Liquid Chromatography

“…Manymethodshave been introduced todetection and measurement of methamphetamine till now. There are several methods for measurement of methamphetamine concentration in various samples including HPLC using fluorescence detection [16][17][18] , HPLC with chemiluminescence detection 19,20 , gas chromatography-mass spectrometry 21,22 , gas chromatography with nitrogen-phosphorus detectio 23 , colourimetric detection using digital and mobile phone technology 24 , andelectrochemistry techniques such as electrochemiluminescence 25,26 .…”

All Eyes on Visible‐Light Peroxyoxalate Chemiluminescence Read‐Out Systems

“…[31] Compared to fluorescenced etection, CL detection does not requirep hotoexcitation. Due to the lack of ap hotoexcitation sourcea nd the baseline error associated with it, CL is often much more sensitive at detecting analytes.P O-CL/HPLC has been employed to detect traces of methamphetamine-based compounds in rat hair roots, [32] halofantrine-an anthracenemethanold erivativeu sed to treat malaria-in rat plasma, [33] catecholamines in mice brain samples [34] and human plasma [35] amongst many other applications.…”