Evaluation of histological appearance of tissues removed by cavitron ultrasonic surgical aspirator (CUSA)

Malhotra, Veena; Malik, Rena D.; Gondal, Ranjana; Beohar, P. C.; Parkash, B.

doi:10.1007/bf01401235

Cited by 27 publications

(10 citation statements)

References 5 publications

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“…The patients had received no prior treatment to craniotomy. Recurrent tumors, which are known to change the expression of a number of proteins were not included in the study as well as tumors removed exclusively by ultrasonic aspiration since this procedure is known to cause tissue degeneration [21]. The material has been used to investigate the prognostic potential of other markers [4, 22, 23].…”

Section: Methodsmentioning

confidence: 99%

Expression and Prognostic Value of Oct-4 in Astrocytic Brain Tumors

et al. 2016

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BackgroundGlioblastomas are the most frequent type of malignant primary brain tumor with a median overall survival less than 15 months. Therapy resistance of glioblastomas has been attributed to the presence of tumor initiating stem-like cells (TSCs). TSC-related markers have therefore been suggested to have promising potentials as prognostic markers in gliomas.Methodology/Principal FindingsThe aim of the present study was to investigate the expression and prognostic impact of the TSC-related marker Oct-4 in astrocytic brain tumors of increasing grade. In total 114 grade II, III and IV astrocytic brain tumors were immunohistochemically stained for Oct-4, and the fraction and intensity of Oct-4 positive cells were determined by morphometric analysis of full tumor sections. Oct-4 was expressed in all tumors, and the Oct-4 positive cell fraction increased with tumor grade (p = 0.045). There was no association between survival and Oct-4 positive cell fraction, neither when combining all tumor grades nor in analysis of individual grades. Oct-4 intensity was not associated with grade, but taking IDH1 status into account we found a tendency for high Oct-4 intensity to be associated with poor prognosis in anaplastic astrocytomas. Double immunofluorescence stainings showed co-localization in the perivascular niches of Oct-4 and two other TSC markers CD133 and nestin in glioblastomas. In some areas Oct-4 was expressed independently of CD133 and nestin.ConclusionsIn conclusion, high Oct-4 fraction was associated with tumor malignancy, but seemed to be without independent prognostic influence in glioblastomas. Identification of a potential prognostic value in anaplastic astrocytomas requires additional studies using larger patient cohorts.

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Section: Methodsmentioning

confidence: 99%

Expression and Prognostic Value of Oct-4 in Astrocytic Brain Tumors

et al. 2016

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“…Due to their disaggregated nature, the aspirated cellular specimens are usually discarded. However, ultrasonic aspirations have been previously noted to yield viable tumor cells [1][2][3][4], with immunoreactivity for vimentin, S-100 protein, and glial fibrillary acidic protein (GFAP) [5], that are appropriate for potential diagnostic utility [6,7]. However, functional competence of ultrasonically aspirated cells, other than growth in culture, has not been determined.…”

Section: Introductionmentioning

confidence: 99%

Tumor cells from ultrasonic aspirations of glioblastomas migrate and form spheres with radial outgrowth

et al. 2007

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Studies of primary cells from malignant brain tumors such as glioblastomas are limited by the small size of surgically resected specimens. However, glioblastomas are also frequently debulked via ultrasonic aspiration. In this study, we examined the functional competence and growth of their aspirated cells. Cells from minced tissue and aspirations were comparable in migration, formation of pseudopodia, development of cellular spheres with radial outgrowth, and neuroectodermal features. Cultures were maintained for more than six weeks without fibroblastic overgrowth. Our observations show that ultrasonically aspirated specimens contain cells useful for studies of tumor migration and growth of tumorspheres.

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“…Even though CUSA is typically used as a tissue disintegration device, our results show the recovery of diagnostic-quality tissue, reinforcing previous reports. 36–38 …”

Section: Discussionmentioning

confidence: 99%

Development of Stereotactic Mass Spectrometry for Brain Tumor Surgery

Agar¹,

Golby²,

Ligon³

et al. 2011

Neurosurgery

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BACKGROUND Surgery remains the first and most important treatment modality for the majority of solid tumors. Across a range of brain tumor types and grades, postoperative residual tumor has a great impact on prognosis. The principal challenge and objective of neurosurgical intervention is therefore to maximize tumor resection while minimizing the potential for neurological deficit by preserving critical tissue. OBJECTIVE To introduce the integration of desorption electrospray ionization mass spectrometry into surgery for in vivo molecular tissue characterization and intraoperative definition of tumor boundaries without systemic injection of contrast agents. METHODS Using a frameless stereotactic sampling approach and by integrating a 3-dimensional navigation system with an ultrasonic surgical probe, we obtained image-registered surgical specimens. The samples were analyzed with ambient de-sorption/ionization mass spectrometry and validated against standard histopathology. This new approach will enable neurosurgeons to detect tumor infiltration of the normal brain intraoperatively with mass spectrometry and to obtain spatially resolved molecular tissue characterization without any exogenous agent and with high sensitivity and specificity. RESULTS Proof of concept is presented in using mass spectrometry intraoperatively for real-time measurement of molecular structure and using that tissue characterization method to detect tumor boundaries. Multiple sampling sites within the tumor mass were defined for a patient with a recurrent left frontal oligodendroglioma, World Health Organization grade II with chromosome 1p/19q codeletion, and mass spectrometry data indicated a correlation between lipid constitution and tumor cell prevalence. CONCLUSION The mass spectrometry measurements reflect a complex molecular structure and are integrated with frameless stereotaxy and imaging, providing 3-dimensional molecular imaging without systemic injection of any agents, which can be implemented for surgical margins delineation of any organ and with a rapidity that allows real-time analysis.

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Evaluation of histological appearance of tissues removed by cavitron ultrasonic surgical aspirator (CUSA)

Cited by 27 publications

References 5 publications

Expression and Prognostic Value of Oct-4 in Astrocytic Brain Tumors

Expression and Prognostic Value of Oct-4 in Astrocytic Brain Tumors

Tumor cells from ultrasonic aspirations of glioblastomas migrate and form spheres with radial outgrowth

Development of Stereotactic Mass Spectrometry for Brain Tumor Surgery

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