Evaluation of increased incubation temperature and cefixime–tellurite treatment for the isolation of Escherichia coli O157:H7 from minced beef

Doğan, Hilal B.; Kuleaşan, Hakan; Çakır, İbrahim; Halkman, A. Kadir

doi:10.1016/s0168-1605(03)00045-x

Cited by 8 publications

(5 citation statements)

References 16 publications

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“…(Donovan and van Netten 1995). Cefixime-tellurite media has been used for isolating organisms from minced beef (Dogan et al 2003), rectal swabs of cattle (Yilmaz et al 2002), raw vegetables (Fujisawa et al 2002), and sprouts (Fujisawa et al 2000).…”

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confidence: 99%

A glimpse on biological activities of tellurium compounds

Cunha

Gouvêa

Juliano

2009

An. Acad. Bras. Ciênc.

166

113

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Tellurium is a rare element which has been regarded as a toxic, non-essential trace element and its biological role is not clearly established to date. Besides of that, the biological effects of elemental tellurium and some of its inorganic and organic derivatives have been studied, leading to a set of interesting and promising applications. As an example, it can be highlighted the uses of alkali-metal tellurites and tellurates in microbiology, the antioxidant effects of organotellurides and diorganoditellurides and the immunomodulatory effects of the non-toxic inorganic tellurane, named AS-101, and the plethora of its uses. Inasmuch, the nascent applications of organic telluranes (organotelluranes) as protease inhibitors and its applications in disease models are the most recent contribution to the scenario of the biological effects and applications of tellurium and its compounds discussed in this manuscript.

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mentioning

confidence: 99%

A glimpse on biological activities of tellurium compounds

Cunha

Gouvêa

Juliano

2009

An. Acad. Bras. Ciênc.

166

113

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“…The main enrichment protocol factors are the enrichment broth (basal medium), the addition of antibiotics, the temperature, and the length of the enrichment period. In previous studies, Trypticase soy broth (TSB) and E. coli broth (EC) have frequently been used as basal media, often with one antibiotic added (novobiocin in most studies) (1,20,22,24,41,58). For incubation, the enrichment broth is most often incubated at 37°C for 16 to 24 h (1,22).…”

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confidence: 99%

“…In previous studies, Trypticase soy broth (TSB) and E. coli broth (EC) have frequently been used as basal media, often with one antibiotic added (novobiocin in most studies) (1,20,22,24,41,58). For incubation, the enrichment broth is most often incubated at 37°C for 16 to 24 h (1,22). Nevertheless, some authors have reported an optimal growth temperature for E. coli O157:H7 of around 40°C (31,44), and in recent studies workers have also used this temperature for incubation of enrichment cultures (1,2,32).…”

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confidence: 99%

Modeling and Predicting the Simultaneous Growth of Escherichia coli O157:H7 and Ground Beef Background Microflora for Various Enrichment Protocols

Vimont

Vernozy-Rozand

Montet

et al. 2006

Appl Environ Microbiol

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The simultaneous growth of Escherichia coli O157:H7 (O157) and the ground beef background microflora (BM) was described in order to characterize the effects of enrichment factors on the growth of these organisms. The different enrichment factors studied were basal medium (Trypticase soy broth and E. coli broth), the presence of novobiocin in the broth, and the incubation temperature (37°C or 40°C). BM and O157 kinetics were simultaneously fitted by using a competitive growth model. The simple competition between the two microfloras implied that O157 growth stopped as soon as the maximal bacterial density in the BM was reached. The present study shows that the enrichment protocol factors had little impact on the simultaneous growth of BM and O157. The selective factors (i.e., bile salts and novobiocin) and the higher incubation temperature (40°C) did not inhibit BM growth, and incubation at 40°C only slightly improved O157 growth. The results also emphasize that when the level of O157 contamination in ground beef is low, the 6-h enrichment step recommended in the immunomagnetic separation protocol (ISO EN 16654) is not sufficient to detect O157 by screening methods. In this case, prior enrichment for approximately 10 h appears to be the optimal duration for enrichment. However, more experiments must be carried out with ground beef packaged in different ways in order to confirm the results obtained in the present study for non-vacuum-and non-modified-atmospherepacked ground beef.

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“…However, the addition of antibiotics can also inhibit some stressed STEC O157 cells from growing, potentially resulting in false‐negative results (Hussein and Bollinger, 2008). It is not uncommon for research laboratories that commonly rely on primary isolation to use a reduced amount of the recommended antibiotics in their enrichment or plating media (Dogan et al, 2003; Durso et al, 2005b; Vimont et al, 2006; Tillman et al, 2012). For the most thorough recovery of STEC from samples, multiple enrichments can be run with and without antibiotics.…”

Section: Shiga Toxigenic Escherichia Coli O157 Isolationmentioning

confidence: 99%

Primary Isolation of Shiga ToxigenicEscherichia colifrom Environmental Sources

Durso

2013

J. Environ. Qual.

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Since the time of the first microbe hunters, primary culture and isolation of bacteria has been a foundation of microbiology. Like other microbial methods, bacterial culture and isolation methodologies continue to develop. Although fundamental concepts like selection and enrichment are as relevant today as they were over 100 yr ago, advances in chemistry, molecular biology and bacterial ecology mean that today's culture and isolation techniques serve additional supporting roles. The primary isolation of Shiga toxigenic (STEC) from environmental sources relies on enriching the target while excluding extensive background flora. Due to the complexity of environmental substrates, no single method can be recommended; however, common themes are discussed. Brilliant Green Bile Broth, with or without antibiotics, is one of many broths used successfully for selective STEC enrichment. Stressed cells may require a pre-enrichment recovery step in a nonselective broth such as buffered peptone water. After enrichment, immunomagnetic separation with serotype specific beads drastically increases the chances for recovery of STEC from environmental or insect sources. Some evidence suggests that acid treating the recovered beads can further enhance isolation. Although it is common in human clinical, food safety, and water quality applications to plate the recovered beads on Sorbitol MacConkey Agar, other chromogenic media, such as modified CHROMagar, have proven helpful in field and outbreak applications, allowing the target to be distinguished from the numerous background flora. Optimum conditions for each sample and target must be determined empirically, highlighting the need for a better understanding of STEC ecology.

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Evaluation of increased incubation temperature and cefixime–tellurite treatment for the isolation of Escherichia coli O157:H7 from minced beef

Cited by 8 publications

References 16 publications

A glimpse on biological activities of tellurium compounds

A glimpse on biological activities of tellurium compounds

Modeling and Predicting the Simultaneous Growth of Escherichia coli O157:H7 and Ground Beef Background Microflora for Various Enrichment Protocols

Primary Isolation of Shiga ToxigenicEscherichia colifrom Environmental Sources

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