The rapid development of nanotechnology paved the way for further expansion of polymer chemistry and the fabrication of advanced polymeric membranes. Such modifications allowed enhancing or adding some unique properties, including mechanical strength, excellent biocompatibility, easily controlled degradability, and biological activity. This chapter discusses various applications of polymeric membranes in three significant areas of biomedicine, including tissue engineering, drug delivery systems, and diagnostics. It is intended to highlight here possible ways of improvement the properties of polymeric membranes, by modifying with other polymers, functional groups, compounds, drugs, bioactive components, and nanomaterials.