Evaluation of <i>in Vitro</i> Bactericidal Activity of 1.5% Olanexidine Gluconate, a Novel Biguanide Antiseptic Agent

Abstract

Recently, 1.5% olanexidine gluconate, a biguanide compounds, was launched as a new antiseptic agent in Japan. However, the comprehensive bactericidal spectrum of olanexidine gluconate is still unknown. In this study, we evaluated in vitro bactericidal activity of olanexidine gluconate using time-kill assay against various bacteria, mycobacteria, and fungi. With the exception of Burkholderia cepacia and Mycobacterium spp., 1.5% olanexidine gluconate exhibited fast-acting (≤60 s) bactericidal activity against all tested Gram-positive and Gram-negative bacteria, including vancomycin-resistant Enterococcus faecalis, methicillin-resistant Staphylococcus aureus, methicillin-resistant Staphylococcus epidermidis, extended spectrum β-lactamase producing Klebsiella pneumoniae, and multidrug-resistant Pseudomonas aeruginosa. Furthermore, 1.5% olanexidine gluconate eradicated Candida albicans, Microsporum canis, and Malassezia furfur within 3 min. Our findings indicate that olanexidine gluconate has broad spectrum bactericidal activity; therefore, it may be useful for the prevention of a wide range of infectious diseases.

In vitro growth-inhibitory effects of Portulaca oleracea L. formulation on intestinal pathogens

“…We measured growth-inhibitory effects of GSC against various bacteria and fungi using the methods that were confirmed by previous studies [10,11] (Fig. 1…”

“…Anaerobic bacteria were cultured under the recommended conditions. Antimicrobial susceptibility and inhibitory effects against these strains were confirmed by previous study [10,11]. GSC (Lot, 2020.1/FCA IK; Iskra Co. Ltd., Tokyo, Japan) was employed as the P. oleracea formulation and contained 3 g (dry weight) of condensed P. oleracea extract per gram and no other bioactive components.…”

“…We measured growth-inhibitory effects of GSC against various bacteria and fungi using the methods that were confirmed by previous studies [10, 11] (). GSC significantly reduced the growth rates of Bacillus subtilis , Escherichia coli , Citrobacter freundii , Shigella boydii , Shigella sonnei , and Salmonella Enteritidis ( P <0.01 for all) and inhibited the growth of Staphylococcus aureus , Klebsiella pneumoniae , Proteus mirabilis , Vibrio cholerae , and Shigella dysenteriae .…”

A case of allergic contact dermatitis caused by Olanedine solution—A diagnostic patch‐testing method involving dried filter paper

“…Olanedine solution (1.5%) (pH ~5, specific gravity ~1.0), with its main component olanexidine gluconate, was launched as a new antiseptic agent in 2015, but it is currently only commercially available in Japan. Several studies 1,2,11 have demonstrated that it exhibits fast‐acting and long‐lasting antimicrobial activity. The bactericidal activity of Olanedine solution persists for 12 hours after the solution is used for antiseptic rinsing, whereas other antiseptics, such as 0.5% chlorhexidine gluconate and 10% povidone‐iodine, hardly display any long‐lasting effectiveness 11 .…”

“…It first became commercially available in Japan in 2015. It has marked bactericidal effects against a wide range of bacteria, including methicillin‐resistant Staphylococcus aureus , vancomycin‐resistant enterococci, and Pseudomonas aeruginosa 1,2 . The active ingredient of Olanedine solution is olanexidine gluconate (Figure 1) (CAS no.…”