Evaluation of Medical Waste Management During the Covid-19 in Diyala Governorate

Introduction and Aim: Thyroid eye illness is widely recognized as one of the most significant concerns confronting the medical profession today. The thyroid-related ophthalmopathy also known as Graves’ ophthalmopathy is an autoimmune disorder that cannot be reversed and hence, research pertaining to the identification of novel markers that can explain both the prognosis and the recovery of the condition is needed. In this study, we aimed to investigate the IL-38 gene expression levels among Graves’ ophthalmopathy patients in Iraq. Methodology: The TSH levels were measured with an enzyme-linked immunosorbent assay (ELISA). The level of IL-38 gene expression in patient blood samples and normal healthy controls was measured qualitatively using RT-PCR. Results: Patients who were diagnosed with Graves’ ophthalmopathy were observed to have abnormally low levels of thyrotropin (TSH) in their serum. In these patients, the interleukin IL-38 gene expression was observed to be significantly greater compared to healthy controls (P<0.01), the tendency of which continued even after one year of therapy with anti-thyroid drugs. Conclusion: The findings of this study indicate that the IL-38 transcript is important in the autoimmune response. The identification of IL-38 expression levels could contribute in the early clinical diagnosis and treatment of thyroid eye disease

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“…There were two identical reactions that were carried out. PCR applied in different area (11)(12)(13)(14)(15)(16)(17).…”

Section: Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

IL-38 gene expression: A new player in Graves’ ophthalmopathy patients in Iraq

Jawad¹,

Numan²,

Ahmed³

et al. 2023

Biomedicine

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Bioremediation by bacteria isolated from water contaminated with hydrocarbons

Fahim Muhsin,

Fadhil AboKsour,

Hadi

2023

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Oil pollution is currently a global problem. However, an oil-contaminated ecology is rich in microorganisms that may utilize petroleum oil and hydrocarbons for growth, feeding, and metabolic processes. In the present study, fifty polluted water samples were collected from five stations (ten samples each) in the Al-Fahama oil refinery in eastern Baghdad. The water contamination parameters of these collected water samples were detected. Then, the percentage of water contamination with some heavy metals (zinc, lead, and cadmium) and radioactive elements (uranium, cesium and actinium) was measured. The proportions of these elements were compared within their limits permitted by the World Health Organization (WHO). Fifty-nine bacterial isolates were isolated from polluted water, and 24 isolates of them succeeded in analyzing crude oil. The results of the current study showed that seven isolates belong to the genus Citrobacter amalonaticus (29.16%), six isolates belong to Enterobacter cloacae (25%), three isolates belonged to both Pseudomonas aeruginosa (12.5%) and Ochrobacterum anthropi (12.5%), and human Ochrobacterum. With a percentage of 12.5%, two isolates of Serratia marcescens (8.3%) and one isolate of each Pseudomonas fluorescens, Serratia fonticola, and Burkholderia pseudomallei (4.16%) of each. The optimum of some conditions for the decomposition process was determined in terms of (pH, temperature and crude oil concentration) and the results showed that the optimum degradation conditions were 35°C at pH equal to 7.5 in the presence of 2% of crude oil. Several experiments were conducted to determine the most efficient isolates for oil analysis. Burkholderia pseudomallei and Pseudomonas fluorescens are the most active bacterial species in their oil degradation. Genes responsible for hydrocarbon analysis were revealed in twenty-four bacterial isolates using a polymerase chain reaction (PCR) assay. The results showed that the ALKB gene (alkane hydroxylase) was observed in all bacterial isolates that succeeded in analyzing crude oil with a percentage equal to 100%, NahAc gene (naphthalene dioxygenase) has been recorded in four isolates (16.7%), these four bacterial isolates were Burkholderia pseudomallei, Pseudomonas aeruginosa, Ochrobacterum anthropic, and Pseudomonas fluorescens. Generally, the isolation rate of both C. amalonaticus and E. cloacae isolates was higher than in other studies, which may be due to the hydrocarbon pollution in isolation; both B. pseudomallei and P. fluorescens isolates were the highest active bacterial species in their oil degradation. Genetic results showed that the AlkB gene was the domain compared with other degradation genes used in the current study, followed by NahAc gene. Keywords: Bioremediation, heavy metal, B. pseudomallei, hydrocarbons, crude oil

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The Immunohistochemical Staining of Vimentin and E-Cadherin in Bladder Cancer Patients Infected with Hepatitis C Virus

Ismael¹,

Qaddoori²,

Sha'ban³

et al. 2023

J. Pure Appl. Microbiol.

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The invasion and spread of cancer cells are two of the most notable characteristics of malignant tumors. Recent studies suggest that the epithelial-mesenchymal transition (EMT) has been linked to this significant occurrence. It is linked to the absence of the epithelial brow and the presence of mesenchymal facial hair. The aims of the present study were to explore the immunohistochemical staining of vimentin and E-cadherin ex vivo as EMT markers and assess their potential as predictive biomarkers for transitional cell cancer (TCC). In this study, 55 paraffin-embedded biopsies from TCC patients and 10 autopsies that appeared to be normal were included. Immunohistochemistry was used to produce patterns of vimentin and E-cadherin expression. When compared to female patients, the expression of E-cadherin and vimentin significantly increased with increasing age in male patients (> 50 years). In contrast to the considerable rise in vimentin expression in higher grades and stages of the tumor, E-cadherin expression was significantly reduced with tumor stage and grade. The findings of this study reveal that elevated vimentin and reduced E-cadherin are important indicators associated with a poor prognosis for TCC.

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Evaluation of Medical Waste Management During the Covid-19 in Diyala Governorate

Cited by 3 publications

References 0 publications

IL-38 gene expression: A new player in Graves’ ophthalmopathy patients in Iraq

IL-38 gene expression: A new player in Graves’ ophthalmopathy patients in Iraq

Bioremediation by bacteria isolated from water contaminated with hydrocarbons

The Immunohistochemical Staining of Vimentin and E-Cadherin in Bladder Cancer Patients Infected with Hepatitis C Virus

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