Evaluation of micro-organism-detaching efficacy from meat samples by spindle or stomacher treatment and quality analysis of suspensions

Kim, Soo‐Ji; Kim, Do‐Kyun; Kang, Dong‐Hyun

doi:10.1111/jam.13075

Cited by 5 publications

(2 citation statements)

References 14 publications

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“…However, no phages were recovered from the stomached samples. It is important to note that Stomachers are designed for roughly homogenising (typically food) samples in order to permit microbiological profiling [25]. Therefore, they are not an appropriate apparatus to generate the desired peristaltic force.…”

Section: Discussionmentioning

confidence: 99%

Alginate-Encapsulated Mycobacteriophage: A Potential Approach for the Management of Intestinal Mycobacterial Disease

O’Connell,

Coffey,

O’Mahony

2023

Viruses

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Encapsulated medication is a common method of administering therapeutic treatments. As researchers explore alternative therapies, it is likely that encapsulation will remain a feature of these novel treatments, particularly when routes of delivery are considered. For instance, alginate-encapsulation is often favoured where gastric digestion poses an obstacle. When exposed to cations (namely Ca2+), alginate readily forms gels that are resilient to acidic conditions and readily dissociate in response to mid-range pH. This action can be extremely valuable for the encapsulation of phages. The efficient delivery of phages to the intestine is important when considering mycobacteriophage (MP) therapy (or MP prophylaxis) for disseminated mycobacterial infections and chronic gastroenteritis conditions. This study presents the design and in vitro validation of an alginate-encapsulated MP capable of releasing phages in a pH-dependent manner. Ultimately, it is shown that encapsulated phages pretreated with simulated gastric fluid (SGF) are capable of releasing viable phages into simulated intestinal fluid (SIF) and thereby reducing the mycobacterial numbers in spiked SIF by 90%. These findings suggest that alginate encapsulation may be a viable option for therapeutic and prophylactic approaches to the management of intestinal mycobacterial disease, such as Johne’s disease.

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Section: Discussionmentioning

confidence: 99%

Alginate-Encapsulated Mycobacteriophage: A Potential Approach for the Management of Intestinal Mycobacterial Disease

O’Connell,

Coffey,

O’Mahony

2023

Viruses

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“…Overall, the spindle has several advantages over the stomacher and sterile cotton swab. The stomacher disintegrates almost the entire food structure resulting in a turbid suspension, but the spindle produces a very clear diluent which can be used for further studies such as BCA (bicinchoninic acid) protein assay or RT-PCR (Kim et al , 2016). Moreover, a sterile cotton swab has less effectiveness for recovery of bacterial cell populations and takes considerable time and energy.…”

Section: Discussionmentioning

confidence: 99%

Comparison of spindle and stomacher efficacy for detaching biofilms from stainless steel, PVC, and green leafy vegetable surfaces

Kim

Jeong

Cheon

et al. 2019

BFJ

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Purpose Biofilms are bacterial communities embedded in exopolysaccharide, enhancing the difficulty of detaching bacterial cells from surfaces. Due to structural properties, it is difficult to detach biofilms. Many removal methods have been developed, but there are still some limitations such as sample size and reproducibility. “Spindle” was developed, producing a higher quality suspension which can be used for further study. The paper aims to discuss these issues. Design/methodology/approach The authors compared the enumeration of biofilm-forming cells detached from the spindle and stomacher in various surfaces. First, the authors chose stainless steel and polyvinyl chloride to attach biofilms and to be subjected to stomacher and spindle for up to 2 min. Also, the authors evaluated the efficiency of detachment from vegetable surfaces. Findings In a comparative experiment of abiotic surfaces, the spindle showed identical effectiveness for detaching biofilm-forming cells compared to the stomacher, recovering the population by 8-log for Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytogenes. The spindle also showed no significant difference from the stomacher in the number of recovered cells which is 4-log from vegetable surfaces. However, turbidity after spinach was subjected to spindle was 4.37 NTU, while it was 99 NTU for stomacher, which was in accord with visual result about clearance. Originality/value This study demonstrated that the spindle is a useful to separate biofilms from surfaces without destructing structure, and thus it can be used for analysis in food laboratories as well as utilized for vegetable washing step in the food industry.

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Designing primer-probe sets for Escherichia coli and E. coli O157 detection: Comparative genomic approach and food interference assessment

Yong,

Lee,

Kang

2024

Food Bioscience

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Evaluation of micro-organism-detaching efficacy from meat samples by spindle or stomacher treatment and quality analysis of suspensions

Abstract: The Spindle could be an alternative method for detaching micro-organisms, yielding a higher quality of suspensions which may be better suited for further molecular microbiological analysis.

Cited by 5 publications

References 14 publications

Alginate-Encapsulated Mycobacteriophage: A Potential Approach for the Management of Intestinal Mycobacterial Disease

Alginate-Encapsulated Mycobacteriophage: A Potential Approach for the Management of Intestinal Mycobacterial Disease

Comparison of spindle and stomacher efficacy for detaching biofilms from stainless steel, PVC, and green leafy vegetable surfaces

Designing primer-probe sets for Escherichia coli and E. coli O157 detection: Comparative genomic approach and food interference assessment

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