“…Several imaging techniques, such as magnetic resonance imaging (MRI) (Ghosh, Jayas, Gruwel, & White, 2006), synchrotron X-ray fluorescence (De Jonge & Vogt, 2010), X-ray absorption (Staedler, Masson, & Schönenberger, 2013), phase contrast (Cloetens, Mache, Schlenker, & Lerbs-Mache, 2006) and neutron tomography (Cleveland IV et al, 2008), have been applied to plant and seed analysis. Among the techniques, high-resolution desktop X-ray absorption tomography (micro-CT) is highly recommended because of its excellent contrast, which allows flexibility to analyze different biological and mineral materials and it increases commercial instrumentation availability (Stuppy, Maisano, Colbert, Rudall, & Rowe, 2003;Pittia et al, 2011;Milien, Renault-Spilmont, Cookson, Sarrazin, & Verdeil, 2012;Dawson, Francis, & Carpenter, 2014;Friis, Marone, Pedersen, Crane, & Stampanoni, 2014). Unlike radiography, which is a projection image, micro-CT produces a pack of two-dimensional cross-sectional images that can be combined into a three-dimensional image allowing internal inspection and measurement (Stuppy et al, 2003).…”