Evaluation of mutagenic effects of formocresol: detection of DNA-protein cross-links and micronucleus in mouse bone marrow

Ramos, Maria Emília Santos Pereira; Cavalcanti, Bruno C.; Costa-Lotufo, Letı́cia V.; Moraes, Manoel Odoríco de; Cerqueira, Eneida de Moraes Marcílio; Pessoa, Cláudia

doi:10.1016/j.tripleo.2007.08.009

Cited by 19 publications

(19 citation statements)

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“…The components of formocresol, especially formaldehyde, have been reported to be associated with cytotoxicity, carcinogenicity, immune sensitization and mutagenicity (Lewis & Chestner , Ramos et al . , Cardoso et al . , Lewis ).…”

Section: Discussionmentioning

confidence: 99%

“…Formocresol has been shown to cause DNA damage in mouse bone marrow (Ramos et al . ), and formocresol induces apoptosis and necrosis in murine peritoneal macrophages (Cardoso et al . ).…”

Section: Discussionmentioning

confidence: 99%

“…Formaldehyde (paraformaldehyde and trioxymethylene), a primary component of formocresol, is recognized as an allergen, which can provoke anaphylactic reactions (Ha€ ıkel et al 2000, Kunisada et al 2002, Braun et al 2003. In addition, the components of formocresol have been reported to be associated with cytotoxicity, carcinogenicity, immune sensitization and mutagenicity (Lewis & Chestner 1981, Ramos et al 2008, Cardoso et al 2010, Lewis 2010.…”

Section: Introductionmentioning

confidence: 99%

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Brain injury due to anaphylactic shock as a result of formocresol used during root canal treatment

et al. 2013

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Section: Discussionmentioning

confidence: 99%

“…Formocresol has been shown to cause DNA damage in mouse bone marrow (Ramos et al . ), and formocresol induces apoptosis and necrosis in murine peritoneal macrophages (Cardoso et al . ).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Brain injury due to anaphylactic shock as a result of formocresol used during root canal treatment

et al. 2013

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“…However, components of FC formulation, especially formaldehyde, have controversial properties, being implicated in the occurrence of periapical changes, alteration of the healing process, systemic distribution in animal organs, and mutagenic effects (Block et al 1983;Soffritti et al 2002;Ramos et al 2008). …”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the cytotoxic effects of FC have been studied in vitro in a variety of systems (Hagiwara et al 2006;Ramos et al 2008), while in vivo, FC treatment promotes necrosis and inflammatory reaction in the connective tissue (Sant' Anna et al 2008). Apoptosis and necrosis have traditionally been addressed as two forms of cell death that exhibit very distinctive biochemical and morphological characteristics (Majno and Joris 1995), but these processes are currently viewed as two extremes of a continuum of possible types of cell demise (Denecker et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Morphological and biochemical changes during formocresol induced cell death in murine peritoneal macrophages: apoptotic and necrotic features

et al. 2010

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The present study was conducted to investigate the role of Formocresol (FC)-induced apoptosis and necrotic cell death in murine peritoneal macrophages (pMø). Macrophages were cultured with 1:100 FC for 2 to 24 h. The viability (trypan blue assay), cell morphology (scanning electronic microscope), and apoptotic and necrotic indexes (light and fluorescent microscopy) were determined at different scheduled times. Simultaneously, the expressions of proteins related to stress, survival, and cell death were measured by western blotting. FC-exposed macrophages exhibited maximal apoptosis from 2 to 6 h, coincident with Bax overexpression (P < 0.001). Additionally, Bcl-x(L) showed maximal expression between 12 and 24 h suggesting its survival effect in pMø. The lowest pMø viability and the increment of the necrotic rate from 4 to 12 h were observed in accordance to Fas and Hsp60 overexpressions. In summary, all the experimental data suggest that two different pathways emerge in pMø exposed to FC, one leading Bax-dependent apoptosis (2-6 h) and the other one favoring necrosis (4-18 h), related to Fas-receptor and Hsp60 stress signal.

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Effect of laser (λ 660 nm) and LED (λ 630 nm) photobiomodulation on formocresol-induced oral ulcers: a clinical and histological study on rodents

et al. 2014

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The aim of this study was to evaluate, clinically and histologically, the influence of laser and LED photobiomodulation in the healing of formocresol-induced oral mucosa ulcers of rats. We used 60 Wistar rats in which oral ulcers were induced on the gingiva of the lower incisors. Forty-eight hours after inducing the ulcers, the animals were divided into three groups: laser, LED, and untreated. Animals from the laser group received irradiation with GaAlAs, 660 nm, CW, 40 mW, φ 4 mm(2), 4.8 J/cm(2). Animals from the LED group received irradiation with InGaAIP, 630 nm, 150 mW, 4.8 J/cm(2), 0.8 cm spot. Forty-eight hours after oral ulcer induction, both irradiations were applied in a punctuate manner in the center of the ulcer at 48-h interval until the end of the experimental period. The animals were killed at 3, 5, 7, and 11 days after day 0. The results of the clinical evaluation showed that the laser and LED phototherapies were able to accelerate the healing of formocresol-induced oral ulcers, which occurred first in the laser group (ANOVA, p < 0.05). Histologically, there was a slight variation between LED and laser therapy; therefore, the laser group proved to be effective in accelerating wound healing, especially at 5 days, whereas the LED group was more effective at the end of the experimental period. It was concluded that laser and LED photobiomodulation were effective in accelerating the healing of formocresol-induced oral ulcers in both clinical and histological aspects.

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Evaluation of mutagenic effects of formocresol: detection of DNA-protein cross-links and micronucleus in mouse bone marrow

Cited by 19 publications

References 50 publications

Brain injury due to anaphylactic shock as a result of formocresol used during root canal treatment

Brain injury due to anaphylactic shock as a result of formocresol used during root canal treatment

Morphological and biochemical changes during formocresol induced cell death in murine peritoneal macrophages: apoptotic and necrotic features

Effect of laser (λ 660 nm) and LED (λ 630 nm) photobiomodulation on formocresol-induced oral ulcers: a clinical and histological study on rodents

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