Evaluation of neutrophil oxidative metabolism in canine monocytic ehrlichiosis

Hasegawa, M. Y.; Kohayagawa, Aguemi; Brandão, Leonardo Pinto; Morgulis, M. S. F. A.; Hagiwara, Mitika K.

doi:10.1111/j.1939-165x.2005.tb00043.x

Cited by 9 publications

(10 citation statements)

References 25 publications

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“…MDA, a breakdown product, which is frequently quantified as a measure of lipid hydroperoxides is a good predictor of oxidative damage. The finding of non-significant increase in the level of MDA in diseased dogs, as observed in the present study, was in corroboration with the earlier in vitro [36] and in vivo [37] studies. This indicates that a minimal role is played by these free radicals in CME disease process.…”

Section: Oxidant/antioxidant Status In Affected Animalssupporting

confidence: 95%

Molecular Detection and Assessment of Hemato-Biochemistry, Oxidant/Antioxidant Status in Natural Canine Monocytic Ehrlichiosis Cases from Northern India

Behera¹,

Dimri

Banerjee

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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The present study was aimed at detecting natural cases of canine monocytic ehrlichiosis (CME) by molecular and cytological methods and assessment of clinico-patho-biochemical parameters among the affected population. Nested polymerase chain reaction was found to be highly sensitive (47.7 %) in detecting the acute cases, followed by buffy coat (29.5 %) and blood smear examination (22.7 %). CME incidence rate was found to be 8.4 %. Hemogram revealed significant (P \ 0.01) depletion in the levels of hemoglobin, hematocrit, total erythrocyte count, monocyte and platelet count in diseased dogs as compared to healthy controls. Plasma biochemistry revealed hypoproteinemia, hypoalbuminemia and hyperglobulinemia along with significantly (P \ 0.05) higher blood urea nitrogen and alanine aminotransferase values. CME revealed non-significant increase in the levels of erythrocytic lipid peroxides, reduced glutathione and activity of superoxide dismutase, whereas activities of glutathione-S-transferase and glutathione reductase showed significant (P \ 0.05) increase and decrease, respectively as compared to healthy controls. Plasma levels of cortisol, insulin and blood level of copper showed non-significant (P [ 0.05) reduction, whereas blood level of zinc showed significant (P \ 0.05) reduction as compared to healthy controls. These findings revealed that changes in erythrocytic oxidant-antioxidant profile and blood mineral concentration in canine monocytic ehrlichiosis are largely nonsignificant and inconsistent. Hence, further study is required to elucidate their role in pathogenesis of canine monocytic ehrlichiosis.

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Section: Oxidant/antioxidant Status In Affected Animalssupporting

confidence: 95%

Molecular Detection and Assessment of Hemato-Biochemistry, Oxidant/Antioxidant Status in Natural Canine Monocytic Ehrlichiosis Cases from Northern India

Behera¹,

Dimri

Banerjee

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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“…Por outro lado esse achado não é o único, uma vez que a infecção por E. canis pode ocasionar supressão difusa da medula óssea, induzindo trombocitopenia, anemia e leucopenia (BUHLES, HUXSOLL, RISTIC, 1974). Valores eritrocitários abaixo do limite inferior também foram descritos por Harrus et al (1996), Castro et al (2004) e Hasegawa et al (2005). Mesmo diante da ausência de associação com sinais clínicos, a apatia e a presença de mucosas pálidas apresentaram-se frequentes entre os animais positivos para E. canis e podem ser consequência dos baixos valores eritrocitários.…”

Section: Exames Hematológicosunclassified

Prevalência da erliquiose monocítica canina e anaplasmose trombocítica em cães suspeitos de hemoparasitose em Cuiabá, Mato Grosso

et al. 2013

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ResumoO presente estudo avaliou a ocorrência da Erliquiose Monocítica Canina e Anaplasmose Trombocitotrópica Canina em 77 cães atendidos no Hospital Veterinário (HOVET) da Universidade Federal de Mato Grosso (UFMT) no ano de 2009, buscando associar à possíveis dados clínicos e hematológicos. Os cães foram avaliados pela amplificação parcial do genes dsb e 16S rRNA de Ehrlichia canis e Anaplasma platys e Reação de Imunofluorescência Indireta (RIFI) para E. canis. DNA de E. canis foi detectado em 18 (23,3%) e de A. platys em 07 (9,1%) cães. Cinquenta e quatro (70,1%) cães foram soropositivos, com títulos variando de 40 a 327.680. Observou-se maior frequência de positivos pela PCR para E. canis nos cães com idade até 12 meses, com anemia, linfopenia e trombocitopenia (P≤ 0,05). Dentre os positivos para A. platys, os cães leucopênicos apresentaram tendência à positividade pela PCR (P=0,07). Segundo os resultados da RIFI, cães apresentando trombocitopenia e hiperproteinemia foram associados a soropositividade (P≤0,05). Dentre os animais positivos pela PCR para E. canis, a linfoadenopatia e alterações pulmonares foram observada em 15 (30,6%) e 4 (57,1%) cães respectivamente (P≤0,05). Outras alterações não apresentaram associação significativa (P>0,05) para E. canis e A. platys. E. canis foi a única espécie de Ehrlichia detectada em cães atendidos no HOVET no ano de 2009, apresentando alta taxa de infecção em cães jovens e estatisticamente associada a cães anêmicos e trombocitopênicos. Por outro lado, A. platys apresentou baixa ocorrência entre os cães analisados. Palavras-chave: Ehrlichia canis, Anaplasma platys, anemia, trombocitopenia, sorologia, PCR AbstractThe present study evaluated Canine Monocytic Ehrlichiosis and Canine Thrombocytic Anaplasmosis in 77 dogs treated at the Veterinary Hospital (HOVET) of the Federal University of Mato Grosso (UFMT) in

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“…In this condition, neutrophils do not produce superoxide due to a failure in enzyme production, causing recurrent infections (Babior et al, 1973). Currently, NBT is being used in several species to assess not only the oxidative metabolism of neutrophils, but also its diagnostic potential for several infectious diseases, including leishmaniosis (Gómez-Ochoa et al, 2010, 2012Hasegawa et al, 2005;Strasser et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Neutrophil dysfunction varies with the stage of canine visceral leishmaniosis

Almeida

Narciso

Bosco

et al. 2013

Veterinary Parasitology

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Canine visceral leishmaniosis (CVL) causes a dependent-stage alteration in neutrophil oxidative metabolism. When production of reactive oxygen species (ROS) exceeds the antioxidant capacity of neutrophils, apoptosis is triggered, impairing the viability and function of these cells, which can predispose dogs to infection. However, the uremic condition observed in late-stage CVL can also alter the viability and function of human neutrophils. To more clearly understand this relationship, the apoptosis rate and oxidative metabolism of neutrophils from control dogs (n=20) were compared to dogs in moderate (n=15) and very severe (n=15) stage CVL, classified according to LeishVet Consensus. To assess neutrophil oxidative metabolism, superoxide production was measured using the nitroblue tetrazolium reduction test (NBT) in isolated neutrophils. The apoptosis rate of neutrophils was estimated using the morphological method. Moderate-stage dogs presented increased superoxide production, while dogs with very severe stage CVL presented decreased superoxide production and an increase neutrophil apoptosis rate. Leishmaniosis causes differential neutrophil dysfunction according to disease stage. In moderate stage CVL, increased superoxide production is observed with no change in neutrophil viability. However, in very severe stage CVL, decreased superoxide production and increased apoptosis occur associated with uremia.

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Evaluation of neutrophil oxidative metabolism in canine monocytic ehrlichiosis

Abstract: Infection by E canis has no influence on neutrophil oxidative metabolism even though during the remission period of the acute phase of the disease, the neutrophils seem to be more reactive under stimulation.

Cited by 9 publications

References 25 publications

Molecular Detection and Assessment of Hemato-Biochemistry, Oxidant/Antioxidant Status in Natural Canine Monocytic Ehrlichiosis Cases from Northern India

Molecular Detection and Assessment of Hemato-Biochemistry, Oxidant/Antioxidant Status in Natural Canine Monocytic Ehrlichiosis Cases from Northern India

Prevalência da erliquiose monocítica canina e anaplasmose trombocítica em cães suspeitos de hemoparasitose em Cuiabá, Mato Grosso

Neutrophil dysfunction varies with the stage of canine visceral leishmaniosis

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