Evaluation of New Film Based on Chitosan/Gold Nanocomposites on Antibacterial Property and Wound‐Healing Efficacy

Abstract

Chitosan (CS) is a natural polymer with applications in wound restorations and coatings for artificial implants. However, the lack of antimicrobial activity restricts its further application. Thus, the purpose of this study is to prepare CS/AuNPs film by adding gold nanoparticles (AuNPs) to modify chitosan. Through transmission electron microscopy, we found that the presence of CS can effectively prevent the agglomeration of nanoparticles. Furthermore, through the CCK experiment, we found that AuNPs at a certain concentration can improve the adhesion and proliferation of cells, which has also been proved in animal experiments, including gross appearance, histological observations, and immunofluorescence staining. Such findings support the feasibility of using the film as a promising candidate for tissue engineering of skin in a near future.

The Expanded Role of Chitosan in Localized Antimicrobial Therapy

“…The modification with gold NPs significantly improved the antibacterial activity of the chitosan films against both S. aureus and E. coli. Additionally, the modified film improved wound healing in a noninfected wound model in rats, further proving the potential of these films as wound healing scaffold [241]. Additionally, other inorganic agents have been utilized to improve the antimicrobial potential of chitosan films.…”

Effective Removal of Methylene Blue from Simulated Wastewater Using ZnO-Chitosan Nanocomposites: Optimization, Kinetics, and Isotherm Studies

“…Having the presence of three types of functional groups—amino/acetamido group, primary and secondary hydroxyl groups on its backbone—chitosan is widely used as modifying agents in polymer composites [ 2 , 3 ]. It has shown great potential in industrial applications due to its non-toxic nature, biodegradability, and biocompatibility [ 4 , 5 ]. It has affinity for various organic and inorganic materials due to its abundant reaction sites.…”

“…It can adsorb and chelate metal ions [ 6 ], and interact with bioactive molecules [ 7 ]. Because of its porosity, biocompatibility, biodegradability, non-toxicity, and robustness, it has versatile application in the preparations of novel chitosan-based materials for various applications such as pharmaceutical, nutraceutical [ 8 ], biomedical [ 9 , 10 ], fertilizer delivery [ 11 ], CO 2 capture [ 5 ], catalysis [ 12 ] and wastewater remediations [ 13 , 14 ].…”

The Effect of Nanoparticle-Incorporated Natural-Based Biomaterials towards Cells on Activated Pathways: A Systematic Review

“…Similarly, the presence of nanoparticles in a polymeric composite film significantly improved the antibacterial properties against S. aureus and E. coli . The film was also allowed enrichment in cell adhesion and proliferation, due to the specific concentration of nanoparticles [ 100 ]. Similarly, the AuNPs promote the expression of cell-related growth factors such as vascular endothelial growth factor (VEGF) and collagen type-1 (COL-1), thus inducing the ECM synthesis; this, in turn, provides a more conducive environment for the cells [ 101 , 102 ].…”