Evaluation of new medium with chromogenic substrates for members of the family Enterobacteriaceae in urine samples

Kodaka, Hidemasa; Ishikawa, Mariko; Iwata, Morihiro; Kashitani, F; Mizuochi, Shingo; Yamaguchi, Keizo

doi:10.1128/jcm.33.1.199-201.1995

Cited by 24 publications

(11 citation statements)

References 10 publications

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“…Media with chromogens, on which the colonies rather than the surrounding medium is stained, easily allow for the detection of the target organisms even in mixed cultures and at low colony counts (17). However, most of the studies performed with these media did not investigate the recovery of rarer organisms (15,(18)(19)(20)(21)(22)(23).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of BBL CHROMagar orientation medium for detection and presumptive identification of urinary tract pathogens

Hengstler¹,

Hammann²,

Fahr³

1997

J Clin Microbiol

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The microbiological performance of BBL CHROMagar Orientation medium and CPS ID2 agar was compared to that of Columbia agar with 5% sheep blood and MacConkey agar without crystal violet for the enumeration and presumptive identification of bacteria responsible for urinary tract infections. Of a total of 658 clinical urine specimens, 118 specimens yielded no growth, 402 specimens yielded growth with cell counts of >10 5 CFU/ml, and 138 specimens yielded growth with cell counts of <10 5 CFU/ml. Of the specimens with cell counts of >10 5 CFU/ml, 163 were pure cultures and 239 were mixed cultures. A total of 266 Escherichia coli organisms were isolated on both chromogenic media, 260 were isolated on blood agar, and 248 were isolated on MacConkey agar. One strain (0.4%) failed to develop the expected pink color on CHROMagar Orientation medium, and 23 strains (8.7%) failed to develop the expected pink color on CPS ID2 agar. Enterococci (CHROMagar Orientation medium, n ‫؍‬ 266; CPS ID2 agar, n ‫؍‬ 265) produced small blue-green colonies on both chromogenic media. Fifty of the mixed cultures contained enterococci that were detected only on the chromogenic media. The Klebsiella-Enterobacter-Serratia (KES) and the Proteus-Morganella-Providencia (PMP) groups could be identified on both chromogenic media. Of 66 isolates of the KES group, 63 grew with the expected color on CHROMagar Orientation medium and 58 of 64 isolates grew with the expected color on CPS ID2 agar. Other microorganisms required further identification. The use of chromogenic medium formulations offers a time-saving method for the reliable detection, enumeration, and presumptive identification of urinary tract pathogens. One of the greatest advantages of these media is the easy recognition of mixed cultures.For several years chromogenic media that allow for the direct identification of the most frequently isolated bacterial and fungal species, such as CPS ID2, SMID agar, and Albicans ID (BioMérieux), Rambach agar (CHROMagar), and BBL CHROMagar Candida (Becton Dickinson), have been available for use with either urine samples or stool specimens or for the identification of yeasts (1,2,9,12,19,22,(24)(25)(26).Since the diagnosis of urinary tract infections contributes significantly to the daily workload in a microbiology laboratory, any innovation that tends to reduce the workload is always welcome when a high-quality standard is still maintained.Our objectives were to evaluate the accuracy of BBL CHROMagar Orientation medium and CPS ID2 agar compared to those of conventional media (Columbia agar with 5% sheep blood and MacConkey agar without crystal violet) for the detection and enumeration of the organisms commonly involved in urinary tract infections and to compare the ability of both chromogenic media to correctly identify the more frequently occurring organisms or organism groups with only a minimum of confirmatory tests.Apart from specimens with clinically significant organism counts (Ն10 5 CFU/ml), we included specimens with Ͻ10 5 CFU/ml in order to evalu...

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Section: Discussionmentioning

confidence: 99%

Evaluation of BBL CHROMagar orientation medium for detection and presumptive identification of urinary tract pathogens

Hengstler¹,

Hammann²,

Fahr³

1997

J Clin Microbiol

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“…The sensitivities rates of P. aeruginosa with CKNA, NAC, PASA, and CET were 88.2%, 81.3%, 79.2%, and 84.0%, respectively. The specificities of CKNA, NAC, PASA, and CET were 99.2%, 98.4%, 99.2%, and 99.7%, respectively.Pseudomonas aeruginosa was the second most commonly isolated organism from urine samples in Toho University Omori Hospital in Tokyo, Japan (KODAKA et al 1995). P. aeruginosa has increased significantly as a human pathogen (HIGHSMITH et al 1985) and has been an important pathogen among patients with compromised immunity, including those with neutropenia, thermal burns, and AIDS (International Pseudomonas aeruginosa Typing Study Group 1994).…”

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confidence: 99%

Evaluation of a new agar medium containing cetrimide, kanamycin and nalidixic acid for isolation and enhancement of pigment production of Pseudomonas aeruginosa in clinical samples

Kodaka

Iwata

Yumoto

et al. 2003

J. Basic Microbiol.

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A new selective agar medium (CKNA), containing cetrimide 0.3 g/l, kanamycin sulfate 50 mg/l, and nalidixic acid 5 mg/l, was developed for the isolation of Pseudomonas aeruginosa. It was compared to Nalidixic Acid Cetrimide agar (NAC), Pseudomonas Aeruginosa Selective Agar (PASA) and Pseudosel(TM) agar (CET) using 1,148 clinical specimens. The sensitivities rates of P. aeruginosa with CKNA, NAC, PASA, and CET were 88.2%, 81.3%, 79.2%, and 84.0%, respectively. The specificities of CKNA, NAC, PASA, and CET were 99.2%, 98.4%, 99.2%, and 99.7%, respectively.

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“…Thus chromogenic agar medium is advocated as an attractive and easy to use primary screening medium that hopefully will considerably reduce the daily workload and limits the use of further identification tests 15,16 .…”

Section: Introductionmentioning

confidence: 99%

Chromogenic Agar Medium : A Versatile Tool for the Diagnosis of UTI

Nahar¹,

Hasan²,

Khatun³

et al. 2018

TAJ: J of Teachers Assoc

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Objective: The present study was done on Chromogenic agar media to identify uropathogens more efficiently by its characteristic colony colour for each of the organism.Methodology: A total 300 sample were collected from Rajshahi Medical College Hospital, Bangladesh. Urine samples of the suspected UTI cases, showing pus cells >5/HPF on microscopic examination were included for urine culture simultaneously onto Chromogenic agar media, Blood agar and MacConkey agar media.Results: Culture yielded 139 (46.33%) bacterial growth among them, 133 (44.33%) showed single organism and remaining 06 (2.00%) showed mixed growth of two organisms in different combinations. It is evident from the present study that both Chromogenic agar media and Blood agar (BA) media supported growth of all 145 bacteria, while MacConkey (MAC) agar yielded 133(91.72%) bacterial growths. The rate of presumptive identification of the isolates was found significantly higher (97.24%) on Chromogenic agar media when compared with the MacConkey agar (80.68%) and Blood agar (27.58%) media. Out of 91 E. coli isolated, 88(96.70%) could be identified differentially on Chromogenic agar media in contrast to 85(93.40%) on MacConkey agar and only 06(06.59%) on Blood agar. Again, all 06 (100%) of the isolate-pairs of mixed growth were identified distinctly on Chromogenic agar media, whereas both Blood agar and MacConkey agar media could revealed only 01(16.66%) of the polymicrobial growth.Conclusion: Chromogenic agar media has been documented for its very high yielding rate, rapid presumptive identification of both single and polymicrobial growths with greater precision and avoidance of biochemical tests for further identification of uropathogens. Thus it can be recommended as primary urine culture medium to be used by the clinical microbiology laboratories.TAJ 2012; 25: 64-71

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Evaluation of new medium with chromogenic substrates for members of the family Enterobacteriaceae in urine samples

Cited by 24 publications

References 10 publications

Evaluation of BBL CHROMagar orientation medium for detection and presumptive identification of urinary tract pathogens

Evaluation of BBL CHROMagar orientation medium for detection and presumptive identification of urinary tract pathogens

Evaluation of a new agar medium containing cetrimide, kanamycin and nalidixic acid for isolation and enhancement of pigment production of Pseudomonas aeruginosa in clinical samples

Chromogenic Agar Medium : A Versatile Tool for the Diagnosis of UTI

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