Evaluation of oral mucosal epithelium in diabetic male patients by exfoliative cytology method

Abstract

BackgroundThe goal of present study is to examine comparatively the epithelium of oral mucosa in persons with diabetes types I and II and the healthy persons by cytomorphometric method.MethodsCytology smear was prepared from oral mucosa epithelium in 24 persons with diabetes and 30 healthy persons were stained by papanicolao method. Its before, from patients were requested acquiring written. The quantitative cytomorphometric characteristics were measured in each group by motic plus 2 software. Also, the qualitative evaluation of cytology slides are performed in three groups.ResultsThere were decrease in the nuclear and cytoplasmic size, (P < 0.001) and increase in the ratio of nuclear to cytoplasm size in buccal mucosal areas (P = 0.001) and tongue (P = 0.011) of diabetic persons compared to the healthy persons. There was no significantly statistical difference in diabetic persons types I and II in buccal mucosal area (P = 0.15) and tongue (P = 0.86) in quantitative characteristics of cytomoerphometry. In nuclear and cytoplasm size, there was a significant statistical difference in persons with diabetes type I and persons of control I and persons with diabetes type II and persons of control II in buccal mucosa and the tongue (P < 0.001). Bilobed or multi-lobed nuleous, karyorrhexis and vacuolization of cytoplasm were more in diabetic persons compared to the healthy ones (P < 0.001).ConclusionDiabetes are effective in creating some quantitative and qualitative cytometric alterations in the oral mucosa but the type of diabetes doesn’t seem to be effective on these changes.

Periodontal Therapy and Gingival Health in Patients with Diabetes Mellitus

“…In our study an increase in NA and parameters of optical density were evident in patients with type 2 DM before and after applied periodontal therapy, a finding similar to other authors (14). Comparing values before and after therapy, higher values were observed before periodontal therapy.…”

“…However, data on gingival tissue is scarce. Therefore in our study, cytomorphometric analysis was performed on smears obtained from gingiva of healthy individuals and patients with type 2 DM before and after periodontal therapy in order to illuminate these changes and open the door to future investigations (13)(14)(15)(16)(17).…”

Cytomorphometric Analysis of Exfoliated Buccal Mucosal Cells in Diabetic Patients

“…At the same time, CyD in DM group is (195.81 ± 27.97) µm and in Control group is (239.74 ± 24.45) µm which showed significant decrease of CD and CyD in DM group compared to Control group (Table-1). This result was similar to the studies carried out by Albertie et al, Parmer et al and Seifi et al 5,12,13 . Age, sex, duration of diabetes and history of medication (Oral / injection) for DM patients had no effect the morphometric changes of the cells.…”

Detection of Cytomorphological Changes in Buccal Mucosal Cells of Type 2 Diabetic Patients Using Oral Exfoliative Cytology

“…The type and pathophysiology of diabetes either type 1 or type 2 do not alter the effect on these cytomorphometric changes. 23 Even though cytological and morphological alterations seen in the oral mucosa of diabetic patient were significant. Yet these cytomorphometric findings are not just associated with diabetes only.…”