Evaluation of oxidative status in patients with brucellosis

Abstract

Oxidative stress can be defined as an increase in oxidants and/or a decrease in antioxidant capacity. We aimed to determine total antioxidant capacity (TAC), total peroxide, malondialdehyde and catalase levels in plasma samples, and calculation of oxidative stress index (OSI) in patients with brucellosis to evaluate their oxidative status using a novel automated method. Sixty-nine patients with brucellosis and 69 healthy control subjects were included in the present study. Plasma levels of total peroxide and malondialdehyde were significantly increased in patients as compared with healthy controls (p<0.001 and p<0.001, respectively). In contrast, TAC level was significantly lower in patients as compared with controls (p<0.001). There was no statistically significant difference between the catalase results of the two groups (p>0.05). OSI level was significantly increased in patients as compared with healthy controls (p<0.001). In conclusion, oxidants were increased and antioxidants were decreased in patients with brucellosis. Oxidative stress was increased in patients with brucellosis.

Evaluation of oxidative status in patients with <i>Fasciola hepatica</i> infection

“…Similar findings were reported by others such as Kaya et al in fascioliasis, 6 Melek et al and Serefhanoglu et al in brucellosis. 18,28 In the present study TOS and OSI were significantly higher in the patients than controls. OSI is the ratio of the total plasma peroxide level to TAC, and it is an indicator of oxidative stress.…”

“…In some diseases, an increase in reactive oxygen species is not the primary cause of the illness; it develops secondarily to the primary disorder and is involved in pathogenesis 14 . There are some studies indicating that oxidative stres plays a role in the pathogenesis of viral hepatitis, sepsis, and bacterial infections [15][16][17][18][19] . Numerous studies demonstrated that in many infectious diseases, a variety of inflammatory cells are activated, which lead to production of reactive oxygen and nitrogen species to kill parasites.…”

Impaired Thiol-Disulfide Balance in Acute Brucellosis

“…They also found increased oxidant capacity and decreased antioxidant parameters before therapy, and they obtained increased antioxidant levels after treatment (36). Therefore, it is well known that brucellosis is involved in enhanced free radical production and a depleted antioxidant system (10,(33)(34)(35)(36). Oxidative burst and oxidative killing in macrophages have a significant and primary role in the control of Brucella pathogens (9).…”

Brusellozisli Sığırlarda Oksidatif Stres İndeksi ve Lipid Profilinin İncelenmesi

“…This study determines the changes in TOC, TAC and lipid profile that may play a role in the pathogenesis of brucellosis. Although there are many studies in the medicine [10,11,[20][21][22] , the number of studies in the veterinarian field, especially on cattle is limited.…”

“…Oxidative stress parameters have been reported to be stimulated while the antioxidant concentration decreased in brucellosis, a chronic [7,24,26] . In studies conducted on patients with diagnosed brucellosis, serum TOC and OSI concentrations were reported to significantly increase, and TAC concentration was reported to decrease [10,11,21] . In this study, we found that Brucella significantly increase TOC (P=0.013) and OSI (P=0.002) concentrations in cattle.…”