Evaluation of plastic scintillators for live imaging of 14C-labeled photosynthate movement in plants

Sugita, Ryohei; Sugahara, Kohei; Kobayashi, Natsuko I.; Hirose, Atsushi; Nakanishi, Teruyuki; Furuta, Etsuko; Sensui, Masaaki; Tanoi, Keitaro

doi:10.1007/s10967-018-6102-z

Cited by 8 publications

(5 citation statements)

References 13 publications

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“…The plants were set on the system after exposure to 14 CO 2 . This picture is reproduced from the studies by Sugita et al 2018 [10] and Sugahara et al 2019 [16].…”

Section: Beta-rays Imagingsupporting

confidence: 65%

“…Recently, we employed plastic scintillators to visualize larger plants ( Figure 2). For example, we obtained an image size of 50 cm × 60 cm for 14 C and 32 P kinetics in plants [10,16]. ( Figure 2).…”

Section: Beta-rays Imagingmentioning

confidence: 99%

“…( Figure 2). For example, we obtained an image size of 50 cm × 60 cm for 14 C and 32 P kinetics in plants [10,16]. Large sizes of the RRIS using plastic scintillators.…”

Section: Beta-rays Imagingmentioning

confidence: 99%

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Recent Advances in Radioisotope Imaging Technology for Plant Science Research in Japan

Suzui

Tanoi

Furukawa

et al. 2019

QuBS

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Soil provides most of the essential elements required for the growth of plants. These elements are absorbed by the roots and then transported to the leaves via the xylem. Photoassimilates and other nutrients are translocated from the leaves to the maturing organs via the phloem. Non-essential elements are also transported via the same route. Therefore, an accurate understanding of the movement of these elements across the plant body is of paramount importance in plant science research. Radioisotope imaging is often utilized to understand element kinetics in the plant body. Live plant imaging is one of the recent advancements in this field. In this article, we recapitulate the developments in radioisotope imaging technology for plant science research in Japanese research groups. This collation provides useful insights into the application of radioisotope imaging technology in wide domains including plant science.

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“…The plants were set on the system after exposure to 14 CO 2 . This picture is reproduced from the studies by Sugita et al 2018 [10] and Sugahara et al 2019 [16].…”

Section: Beta-rays Imagingsupporting

confidence: 65%

Section: Beta-rays Imagingmentioning

confidence: 99%

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Recent Advances in Radioisotope Imaging Technology for Plant Science Research in Japan

Suzui

Tanoi

Furukawa

et al. 2019

QuBS

Self Cite

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“…The carbon dioxide (CO 2 ) assimilation in leaves and subsequent photosynthate partitioning were evaluated radiometrically using radiolabelled CO 2 ( 14 CO 2 ), as previously described by Sugita et al (2018). The 14 CO 2 molecules (0.2 MBq) were generated in a vial by the chemical reaction between 14 C-sodium bicarbonate (200 kBq, PerkinElmer, Waltham, MA, United States) and lactic acid.…”

Section: Determination Of Carbon Assimilation and Photosynthate Partimentioning

confidence: 99%

Short-Term Magnesium Deficiency Triggers Nutrient Retranslocation in Arabidopsis thaliana

et al. 2020

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Magnesium (Mg) is essential for many biological processes in plant cells, and its deficiency causes yield reduction in crop systems. Low Mg status reportedly affects photosynthesis, sucrose partitioning and biomass allocation. However, earlier physiological responses to Mg deficiency are scarcely described. Here, we report that Mg deficiency in Arabidopsis thaliana first modified the mineral profile in mature leaves within 1 or 2 days, then affected sucrose partitioning after 4 days, and net photosynthesis and biomass production after 6 days. The short-term Mg deficiency reduced the contents of phosphorus (P), potassium, manganese, zinc and molybdenum in mature but not in expanding (young) leaves. While P content decreased in mature leaves, P transport from roots to mature leaves was not affected, indicating that Mg deficiency triggered retranslocation of the mineral nutrients from mature leaves. A global transcriptome analysis revealed that Mg deficiency triggered the expression of genes involved in defence response in young leaves.

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“…On the contrary, a previous study showed that the shielding factor of this Al film for 14 C spots was 0.986. 23) The shielding factor for 14 C was less than 1, seemingly because β-ray Table 3 Shielding factor of different shielding materials tested. Images of 32 P spot (8.14 Bq/mm 2 ) was taken using Lumineard-B with and without each shielding material.…”

Section: Shading Materialsmentioning

confidence: 99%

Plastic Scintillators Enable Live Imaging of <sup>32</sup>P-labelled Phosphorus Movement in Large Plants

et al. 2019

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We updated our imaging system, namely real-time radioisotope imaging system (RRIS) to observe 32 P movement in large plants using a large size scintillator. In the previous version of RRIS, the view was limited by the size of a CsI (Tl) scintillator deposited on a fiber optic plate (FOS). However, owing to the high price and difficulty in handling of FOS, the view area was limited to 100×200 mm. In this study, we evaluated 5 large and low-cost plastic scintillators and showed that plastic scintillators can be used in RRIS for the quantitative analysis of 32 P. We also evaluated the resolution of the imaging system with plastic scintillator.

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Evaluation of plastic scintillators for live imaging of 14C-labeled photosynthate movement in plants

Cited by 8 publications

References 13 publications

Recent Advances in Radioisotope Imaging Technology for Plant Science Research in Japan

Recent Advances in Radioisotope Imaging Technology for Plant Science Research in Japan

Short-Term Magnesium Deficiency Triggers Nutrient Retranslocation in Arabidopsis thaliana

Plastic Scintillators Enable Live Imaging of <sup>32</sup>P-labelled Phosphorus Movement in Large Plants

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