Evaluation of reactive oxygen species (ROS) and DNA integrity assessment in cases of idiopathic male infertility

Khodair, Hassan Abou; Omran, Tarek

doi:10.4103/1110-6530.123932

Cited by 4 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Under these circumstances, there is a significant increase in sperm DNA damage in semen samples that exhibit OS, whether OS is measured by ROS or sORP, although this is particularly significant when OS is measured by sORP. There is a wealth of evidence demonstrating an association between sperm DNA damage and OS assessed by chemiluminescence [40,42,43,44,52], but this is the first study to report an increase in sperm DNA damage in samples exposed to OS as determined by the MiOXSYS assay.…”

Section: Discussionmentioning

confidence: 99%

“…Oxidative stress is well known to manifest its effects on semen parameters [28,38,39,40,41,46], as well as playing a major role in sperm DNA damage [8,21,22,27,30,40,42,43,44,54]. In this study, while we have shown that both sORP and ROS are significantly negatively correlated with semen parameters, the correlation is approximately twice as strong for all parameters, with the exception of vitality, when OS is measured by sORP.…”

Section: Discussionmentioning

confidence: 99%

“…In order for an assay to be accepted as a clinically relevant diagnostic test, it should be able to predict abnormalities in markers known to be affected by it. While previous studies of the chemiluminescence and redox assays have shown an association of OS with clinical markers such as semen parameters [28,38,39,40,41] and sperm DNA fragmentation [40,42,43,44,45,46], to date a comparison of the two assays has not been performed. This study presents the first comparison of the two assays to determine which assay is more predictive of impaired semen quality.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Comparison Between Two Assays for Measuring Seminal Oxidative Stress and their Relationship with Sperm DNA Fragmentation and Semen Parameters

Homa

Vassiliou

Stone

et al. 2019

Genes

View full text Add to dashboard Cite

Oxidative stress (OS) is a significant cause of DNA fragmentation and is associated with poor embryo development and recurrent miscarriage. The aim of this study was to compare two different methods for assessing seminal OS and their ability to predict sperm DNA fragmentation and abnormal semen parameters. Semen samples were collected from 520 men attending for routine diagnostic testing following informed consent. Oxidative stress was assessed using either a chemiluminescence assay to measure reactive oxygen species (ROS) or an electrochemical assay to measure oxidation reduction potential (sORP). Sperm DNA fragmentation (DFI) and sperm with immature chromatin (HDS) were assessed using sperm chromatin structure assay (SCSA). Semen analysis was performed according to WHO 2010 guidelines. Reactive oxygen species sORP and DFI are negatively correlated with sperm motility (p = 0.0012, 0.0002, <0.0001 respectively) and vitality (p < 0.0001, 0.019, <0.0001 respectively). The correlation was stronger for sORP than ROS. Reactive oxygen species (p < 0.0001), sORP (p < 0.0001), DFI (p < 0.0089) and HDS (p < 0.0001) were significantly elevated in samples with abnormal semen parameters, compared to those with normal parameters. Samples with polymorphonuclear leukocytes (PMN) have excessive ROS levels compared to those without (p < 0.0001), but sORP and DFI in this group are not significantly increased. DNA fragmentation was significantly elevated in samples with OS measured by ROS (p = 0.0052) or sORP (p = 0.004). The results demonstrate the multi-dimensional nature of oxidative stress and that neither assay can be used alone in the diagnosis of OS, especially in cases of leukocytospermia.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Comparison Between Two Assays for Measuring Seminal Oxidative Stress and their Relationship with Sperm DNA Fragmentation and Semen Parameters

Homa

Vassiliou

Stone

et al. 2019

Genes

View full text Add to dashboard Cite

show abstract

“…Perubahan morfologis pada spermatozoa tidak hanya karena kerusakan struktural membran, namun juga diikuti oleh kerusakan DNA dan subunit ribosom yang mengakibatkan kesalahan translasi (Hallaj Salahipour et al, 2019). Peningkatan kerusakan DNA memiliki korelasi yang kuat terhadap kerusakan struktur dan jumlah kejadian apoptosis pada spermatozoa (Khodair & Omran, 2013).…”

Section: Hasil Dan Pembahasanunclassified

Prevalensi Kejadian Aglutinasi Spermatozoa Rattus norvegicus Strain Wistar Albino yang Diberi Paparan Antibiotik Kanamycin

Abeiasa¹

2020

JRBA

View full text Add to dashboard Cite

Kelompok antibiotik dari golongan aminoglikosida seperti kanamycin dan gentamycin menyebabkan kesalahan penerjemahan dan efek inhibisi pada proses translokasi t-RNA dan m-RNA bakteri. Dilaporkan bahwa toksisitas kanamycin dapat meningkat seiring dengan peningkatan dosis yang diterima. Hal ini dapat berakibat penurunan jumlah dan kecacatan spermatozoa. Penelitian ini bertujuan untuk menguji pengaruh antibiotik kanamycin terhadap prevalensi kejadian aglutinasi spermatozoa. Jumlah aglutinasi spermatozoa dihitung menggunakan agglutination grade WHO. Penelitian ini merupakan penelitian eksperimental dengan model post-test-only control group design dengan variabel independen yaitu kanamycin dosis bertingkat dan variabel dependen yaitu jumlah aglutinasi spermatozoa. Data dianalisis menggunakan ANAVA dan dilanjutkan dengan uji lanjut Bonferroni. Hasil penelitian menunjukkan bahwa pemberian kanamycin dosis bertingkat berpengaruh terhadap prevalensi kejadian aglutinasi spermatozoa. Peningkatan jumlah aglutinasi seiring dengan peningkatan dosis yang diterima.

show abstract

Epidemiology of Oxidative Stress in Male Fertility

Owyong

Ramasamy

2019

Oxidants, Antioxidants and Impact of the Oxidative Status in Male Reproduction

View full text Add to dashboard Cite

Evaluation of reactive oxygen species (ROS) and DNA integrity assessment in cases of idiopathic male infertility

Cited by 4 publications

References 0 publications

A Comparison Between Two Assays for Measuring Seminal Oxidative Stress and their Relationship with Sperm DNA Fragmentation and Semen Parameters

A Comparison Between Two Assays for Measuring Seminal Oxidative Stress and their Relationship with Sperm DNA Fragmentation and Semen Parameters

Prevalensi Kejadian Aglutinasi Spermatozoa Rattus norvegicus Strain Wistar Albino yang Diberi Paparan Antibiotik Kanamycin

Epidemiology of Oxidative Stress in Male Fertility

Contact Info

Product

Resources

About