Evaluation of Resins for Stereolithographic 3D-Printed Surgical Guides: The Response of L929 Cells and Human Gingival Fibroblasts

Kurzmann, Christoph; Janjić, Klara; Shokoohi-Tabrizi, Hassan Ali; Edelmayer, Michael; Pensch, Manuela; Moritz, Andreas; Agis, Hermann

doi:10.1155/2017/4057612

Cited by 48 publications

(37 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This highlights the importance of the relevant culture mode. This is in line with previous studies evaluating the cytotoxicity of 3D printing material [31].…”

Section: Discussionsupporting

confidence: 93%

The impact of 3D-printed LAY-FOMM 40 and LAY-FOMM 60 on L929 cells and human oral fibroblasts

et al. 2020

Self Cite

View full text Add to dashboard Cite

Objectives LAY-FOMM is a promising material for FDA-approved Fused Deposition Modeling (FDM) applications in drug delivery. Here we investigated the impact on oral cells. Materials and methods We evaluated the impact of 3D-printed LAY-FOMM 40, LAY-FOMM 60, and biocompatible polylactic acid (PLA) on the activity of murine L929 cells, gingival fibroblasts (GF), and periodontal ligament fibroblasts (PDLF) using indirect (samples on cells), direct monolayer culture models (cells on samples), and direct spheroid cultures with resazurin-based toxicity assay, confirmed by MTT and Live-dead staining. The surface topography was evaluated with scanning electron microscopy. Results The materials LAY-FOMM 40 and LAY-FOMM 60 led to a reduction in resazurin conversion in L929 cells, GF, and PDLF, higher than the impact of PLA in indirect and direct culture models. Fewer vital cells were found in the presence of LAY-FOMM 40 and 60 than PLA, in the staining in both models. In the direct model, LAY-FOMM 40 and PLA showed less impact on viability in the resazurin-based toxicity assay than in the indirect model. Spheroid microtissues showed a reduction of cell activity of GF and PDLF with LAY-FOMM 40 and 60. Conclusion Overall, we found that LAY-FOMM 40 and LAY-FOMM 60 can reduce the activity of L292 and oral cells. Based on the results from the PLA samples, the direct model seems more reliable than the indirect model. Clinical relevance A material modification is desired in terms of biocompatibility as it can mask the effect of drugs and interfere with the function of the 3D-printed device.

show abstract

“…This highlights the importance of the relevant culture mode. This is in line with previous studies evaluating the cytotoxicity of 3D printing material [31].…”

Section: Discussionsupporting

confidence: 93%

The impact of 3D-printed LAY-FOMM 40 and LAY-FOMM 60 on L929 cells and human oral fibroblasts

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…12 Based on the KH results of this research, when considering the new material, the SG resin showed higher KH than thermopolymerized resin, being the hardest material of these samples, the SG resin indication is to manufacture surgical guide appliances and the material hardness must keep the accuracy and reliability for the guidance. 13 The Dental LT and Clear resin presents hardness lower than Dental SG and thermopolymerized resins,…”

Section: Discussionmentioning

confidence: 98%

The influence of the post-processing method on knoop hardness of photosensitive resins for 3D SLA printer used in Dentistry

Vasques¹,

Mulder²,

Machado³

et al. 2019

Clin Lab Res Dent

View full text Add to dashboard Cite

Objectives: The aim of this study is to compare the mechanical characteristics of resins for 3D printers with the acrylic resins that have been used in Dentistry, according to the post-processing method. Materials and Methods: Using an SLA 3D printer (Form 2 – Formlabs, Massachusetts, USA), samples (discs) were produced with the printer’s resins, Dental SG®, Dental LT®, Clear®, and Grey®. Samples made of thermopolimerized and auto polymerized acrylic resins were produced as well (Gold Standart parameters). The Knoop Hardness (KH) tests were performed using microdurometer HMV-2 (Shimadzu, Kyoto, Japan). Results: The results showed the Dental SG resin presented the highest KH, compared with thermopolimerized acrylic resin, the Clear and Dental LT resins KH were compared with auto polymerized acrylic resin, and the non-post cured Grey resin (not for intraoral use), as well as the Dental LT resin, showed the lowest KH. Conclusions: Considering the hardness, the printer’s resins are comparable with the acrylic resins established, when the post-processing method is thoroughly followed. The absence of material’s post-processing reduce significantly the material’s hardness. Clinical relevance: Regarding the use of 3D printing in Dentistry, the development of materials adequate for the equipment, biocompatible for intraoral uses, and compatible mechanical proprieties are essential.

show abstract

“…Similar complex in vitro models can be developed as screening assays for novel target for periodontal regeneration and the optimization of biomaterials (Xu and Hu, 2017 ). Also scaffold-free approaches of bioprinting seem feasible as spheroids (Janjić et al, 2017 ; Kurzmann et al, 2017 ) and more complex microtissues (Janjić et al, 2017 ) have been generated successfully from periodontal ligament and gingival cells. The application of such self-assembled building blocks for periodontal regeneration has been proposed (Yang et al, 2010 ; Berahim et al, 2011 ).…”

Section: Periodonticsmentioning

confidence: 99%

3D Printing—Encompassing the Facets of Dentistry

Oberoi

Nitsch

Edelmayer

et al. 2018

Front. Bioeng. Biotechnol.

Self Cite

180

125

View full text Add to dashboard Cite

This narrative review presents an overview on the currently available 3D printing technologies and their utilization in experimental, clinical and educational facets, from the perspective of different specialties of dentistry, including oral and maxillofacial surgery, orthodontics, endodontics, prosthodontics, and periodontics. It covers research and innovation, treatment modalities, education and training, employing the rapidly developing 3D printing process. Research-oriented advancement in 3D printing in dentistry is witnessed by the rising number of publications on this topic. Visualization of treatment outcomes makes it a promising clinical tool. Educational programs utilizing 3D-printed models stimulate training of dental skills in students and trainees. 3D printing has enormous potential to ameliorate oral health care in research, clinical treatment, and education in dentistry.

show abstract

Evaluation of Resins for Stereolithographic 3D-Printed Surgical Guides: The Response of L929 Cells and Human Gingival Fibroblasts

Cited by 48 publications

References 26 publications

The impact of 3D-printed LAY-FOMM 40 and LAY-FOMM 60 on L929 cells and human oral fibroblasts

The impact of 3D-printed LAY-FOMM 40 and LAY-FOMM 60 on L929 cells and human oral fibroblasts

The influence of the post-processing method on knoop hardness of photosensitive resins for 3D SLA printer used in Dentistry

3D Printing—Encompassing the Facets of Dentistry

Contact Info

Product

Resources

About