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ABSTRACTThe aim of this study was to assess the combined effects of the three plant growth promoting Rhizobacteria (Azospirillum lipoferum, Pseudomonas fluorescens and Pseudomonas putida) and a biostimulating molecule (chitosan) on plant growth parameters and seeds yield of maize in southern Benin. This study was conducted in reddish ferrous soil with fisher block experimental design. Maize seeds were soaked into chitosan solution for 12 hours and introduced into a seed hole with bacterial suspension. Experimental maize plants were fertilized by half or complete dose of NitrogenPhosphorus-Potassium (NPK) and Urea. Result of the study revealed that the combination of P. fluorescens along with chitosan and half dose of NPK-Urea increased maize height, circumference and seeds yield of 10.18%, 22.05% and 44.10% respectively. Furthermore, the produced biomass increased 71.43% compared to the control in the combination of P. fluorescens, chitosan and complete dose of NPK-Urea. Results of study suggesting that PGPR and chitosan can be used as an effective biological fertilizer combination for increasing maize production under field conditions.