Evaluation of salt tolerance in Nicotiana tabacum plants bearing an antisense suppressor of the proline dehydrogenase gene

Partial suppression of gene encoding proline dehydrogenase enhances plant tolerance to various abiotic stresses

“…According to the earlier data, transgenic tobacco plants mani SR1 no. 8 [21] and higher resistance to salin ization [22] and heavy metals [23]. In most cases, the experiments designed to assess stress resistance were performed in vitro (on MS medium supplemented with stress agents).…”

Elevated proline content in maize plants expressing a fragment of the proline dehydrogenase gene in antisense orientation

“…Proline dehydro genase catalyzes the first stage of proline transforma tion into glutamic acid. When a construct carrying a suppressor of the proline dehydrogenase gene was inserted into tobacco plants, the content of free pro line therein became 1.5-3.0 times higher than in the control plants, and the transformed plants manifested greater stress resistance under laboratory conditions [3,5,6], whereas the transformed arabidopsis plants became more resistant to salinization and low temper atures [7]. A salt resistant canola (Brassica napus L.) genotype with lower activity of proline dehydrogenase became capable of more active proline accumulation as compared with the less resistant genotype [8].…”

“…The increase in proline content is assumed to improve plant resistance to drought and high salt content in soil [2]. For instance, improved nonspecific resistance to salinization and drought was characteristic of tobacco plants with ele vated proline content in plant tissues [3]. Surplus pro line production in transgenic tobacco plants following their transformation with the proline biosynthesis genes proA and proBosm from Escherichia coli improved plant resistance to salt stress [4].…”

“…PCR analysis of total DNA isolated from maize leaves for the antisense sequence of the fragment of the proline dehydrogenase gene (ASPG) (1,3,5). Maize plants containing ASPG; (2) maize plant lacking ASPG; (4) nontransformed maize plant (negative control); (6) plasmid DNA from the LBA4404 strain of A. tumefaciens containing the pBiE2 binary vector (posi tive control); (7) molecular weight marker with an interval of 0.1 kb (Fermentas, Lithuania).…”

Transgenic plants as genetic models for studying functions of plant genes

“…(b) Proline contents in transformant leaves. (c) Transgenic plants were characterized by elevated tolerance to drought, cold, and toxic salts of heavy metals (Kochetov et al, 2004;Kolodyazhnaya et al, 2006;Ibragimova et al, 2012).…”