Evaluation of Secondary Enrichment for Detecting Salmonellae in Bobwhite Quail

Abstract

Secondary enrichment of cultures in tetrathionate-brilliant-green broth substantially increased Salmonella recovery over that achieved with primary tetrathionate-brilliant-green broth or primary selenite-cystine broth.

The Isolation of Salmonellae from Poultry Environmental Samples by Several Enrichment Procedures Using Plating Media with and Without Novobiocin

“…The DSE broth permitted higher recoveries of salmonellae than either of the primary-broth techniques investigated. This observation (increased Salmonella recovery using DSE) is supported by the studies of Pourciau and Springer (1977) and of Rigby and Petit (1980). Both of those studies demonstrated considerably higher recoveries from DSE compared with primary enrichments.…”

“…Cox et al (1980) also did not observe increased recovery from lactose preenrichment broth over direct enrichment broth when examining meat or poultry samples. Pourciau and Springer (1977) analyzed samples from bobwhite quail incubated overnight at 37 C in tetrathionate-brilliant green broth, held at room temperature for 6 to 7 days, then transferred to fresh tetrathionate-brilliant green broth at 37 C. This delayed, secondary enrichment (DSE) resulted in significantly higher recoveries of salmonellae than did direct enrichment. Rigby and Petit (1980) also observed the greater efficacy of DSE broth over preenrichment and direct enrichment 721 by guest on April 10, 2015 http://ps.oxfordjournals.org/ Downloaded from broth for recovering salmonellae samples obtained at poultry houses.…”

Comparison of Conventional and Delayed Secondary Enrichment for Isolation of Salmonella Spp. from Swine Samples

“…7,[10][11][12] For delayed secondary enrichment, samples are inoculated into primary enrichment broth, incubated for 24 hours at 37 C, and held at room temperature for several days. 7,[10][11][12] The primary broth is then used to inoculate tubes of fresh broth, which are routinely incubated for 24 hours and plated onto selective agar. The purpose of this study was to determine if the combination of delayed secondary enrichment and primary enrichment improved Salmonella isolation over that obtained by 24-hour primary enrichment alone.…”

“…7,[10][11][12] After 24 hours' incubation of tetrathionate and 3MC broths at 37 C and Rappaport-Vassiliadis broth at 42 C, the samples were held at room temperature for 5 days, after which 1 ml of each broth was added to a fresh tube containing 9 ml of the same enrichment medium. The tetrathionate and 3MC broths were incubated for 24 hours at 37 C, and the Rappaport-Vassiliadis broth was incubated for 24 hours at 42 C, after which approximately 100 l of each sample was plated onto MacConkey and Hektoen enteric agar plates.…”

“…from bobwhite quail, chickens, chicken environments, and cattle. 7,[10][11][12] This study suggests that 3MC and Rappaport-Vassiliadis broths may also be used for isolation of salmonellae from swine and their environments in a delayed-secondary-enrichment procedure. Recovery of all Salmonella serotypes isolated in this study was improved by delayed secondary enrichment.…”