Evaluation of seminal plasma HSPA2 protein as a biomarker of human spermatogenesis status

Nowicka-Bauer, Karolina; Malcher, Agnieszka; Włoczkowska, Olga; Kamieniczna, Marzena; Olszewska, Marta; Kurpisz, Maciej

doi:10.1016/j.repbio.2021.100597

Cited by 16 publications

(15 citation statements)

References 40 publications

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“…Such unbiased findings align with independent evidence that the overall abundance of HSPA2 in human spermatozoa provides a robust discriminative index of their fertilizing potential (52). By way of a biological explanation, HSPA2 appears to be a critical component of the machinery that orchestrates sperm plasma membrane remodeling events during spermiogenesis and capacitation (56,59,62,(123)(124)(125). Thus, an under-representation of HSPA2 likely has functional consequences in terms of the functional priming of ZP binding domains on the mature sperm surface.…”

Section: Sperm Protein Biomarkerssupporting

confidence: 73%

“…Illustrative of this potential is HSPA2, a molecular chaperone protein previously discussed in relation to its role in the capacitation associated remodeling of the sperm surface, and one that is intimately associated with spermatogenesis. Recent work has also documented the presence of sperm-free HSPA2 in seminal plasma wherein its relative abundance is positively correlated with spermatogenic status (125). It follows that sub-classes of infertile males, such as cryptozoospermic patients, have low to nondetectable seminal plasma HSPA2; likely reflective of an almost total meiosis arrest.…”

Section: Seminal Plasma Biomarkersmentioning

confidence: 97%

“…Beyond the sperm component of the ejaculate, the seminal plasma in which they are bathed is also recognized as comprising a rich variety of biomolecules; many of which could potentially serve as non-invasive biomarkers of a male's fertility status [e.g. proteins (125,184,185), small-molecule metabolites (186), lipids (187), cellfree nucleic acid (DNA, sncRNA and lncRNA) (188)(189)(190)(191), as well as antioxidant agents and inorganic chemicals (ions) (192)]. Indeed, seminal plasma is an elaborate nutrient-rich fluid generated by the accessory glands of the male reproductive tract, which constitutes as much as 95% of human semen (193).…”

Section: Seminal Plasma Biomarkersmentioning

confidence: 99%

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New horizons in human sperm selection for assisted reproduction

Nixon

Schjenken²,

Burke³

et al. 2023

Front. Endocrinol.

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Male infertility is a commonly encountered pathology that is estimated to be a contributory factor in approximately 50% of couples seeking recourse to assisted reproductive technologies. Upon clinical presentation, such males are commonly subjected to conventional diagnostic andrological practices that rely on descriptive criteria to define their fertility based on the number of morphologically normal, motile spermatozoa encountered within their ejaculate. Despite the virtual ubiquitous adoption of such diagnostic practices, they are not without their limitations and accordingly, there is now increasing awareness of the importance of assessing sperm quality in order to more accurately predict a male’s fertility status. This realization raises the important question of which characteristics signify a high-quality, fertilization competent sperm cell. In this review, we reflect on recent advances in our mechanistic understanding of sperm biology and function, which are contributing to a growing armory of innovative approaches to diagnose and treat male infertility. In particular we review progress toward the implementation of precision medicine; the robust clinical adoption of which in the setting of fertility, currently lags well behind that of other fields of medicine. Despite this, research shows that the application of advanced technology platforms such as whole exome sequencing and proteomic analyses hold considerable promise in optimizing outcomes for the management of male infertility by uncovering and expanding our inventory of candidate infertility biomarkers, as well as those associated with recurrent pregnancy loss. Similarly, the development of advanced imaging technologies in tandem with machine learning artificial intelligence are poised to disrupt the fertility care paradigm by advancing our understanding of the molecular and biological causes of infertility to provide novel avenues for future diagnostics and treatments.

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Section: Sperm Protein Biomarkerssupporting

confidence: 73%

Section: Seminal Plasma Biomarkersmentioning

confidence: 97%

Section: Seminal Plasma Biomarkersmentioning

confidence: 99%

See 1 more Smart Citation

New horizons in human sperm selection for assisted reproduction

Nixon

Schjenken²,

Burke³

et al. 2023

Front. Endocrinol.

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“…A reduction of its expression leads to complications with sperm-egg recognition and fertilization, as reviewed in Nixon et al ( Nixon et al, 2015b ). The reduction of HSPA2 protein expression level was observed in seminal plasma samples of men with oligozoospermia as compared to the controls ( Nowicka-Bauer et al, 2022 ), suggesting the possibility of using HSPA2 genes as a biomarker for spermatogenesis status, especially in cryptozoospermic males, and as a predictive biomarker for the success of assisted reproductive techniques (ART) ( Nowicka-Bauer et al, 2022 ). Moreover, the expression of the HSPA2 gene was down-regulated in human testes with abnormal spermatogenesis ( Son et al, 2000 ).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-targeting in male infertility: Sperm microRNA-19a/b-3p and its spermatogenesis related transcripts content in men with oligoasthenozoospermia

Abu‐Halima

Becker

Ayesh

et al. 2022

Front. Cell Dev. Biol.

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Objective: To elucidate and validate the potential regulatory function of miR-19a/b-3p and its spermatogenesis-related transcripts content in sperm samples collected from men with oligoasthenozoospermia.Methods: Men presenting at an infertility clinic were enrolled. MicroRNA (miRNA) and target genes evaluation were carried out using in silico prediction analysis, Reverse transcription-quantitative PCR (RT-qPCR) validation, and Western blot confirmation.Results: The expression levels of miRNA-19a/b-3p were significantly up-regulated and 51 target genes were significantly down-regulated in oligoasthenozoospermic men compared with age-matched normozoospermic men as determined by RT-qPCR. Correlation analysis highlighted that sperm count, motility, and morphology were negatively correlated with miRNA-19a/b-3p and positively correlated with the lower expression level of 51 significantly identified target genes. Furthermore, an inverse correlation between higher expression levels of miRNA-19a/b-3p and lower expression levels of 51 target genes was observed. Consistent with the results of the RT-qPCR, reduced expression levels of STK33 and DNAI1 protein levels were identified in an independent cohort of sperm samples collected from men with oligoasthenozoospermia.Conclusion: Findings suggest that the higher expression of miRNA-19a/b-3p or the lower expression of target genes are associated with oligoasthenozoospermia and male infertility, probably through influencing basic semen parameters. This study lay the groundwork for future studies focused on investigating therapies for male infertility.

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“…Nowicka‐Bauer and collaborators investigated testicular Heat shock‐related 70 kDa protein 2 (HSPA2) as a possible seminal plasma biomarker for spermatogenesis status [56]. They conducted a targeted analysis in cryptozoospermia ( n = 6), NOA ( n = 10), OA ( n = 3), and normozoospermia ( n = 10) cohort.…”

Section: Proteomics Biomarkers For Azoospermiamentioning

confidence: 99%

Proteomics of azoospermia: Towards the discovery of reliable markers for non‐invasive diagnosis

Rusevski

Plaseska‐Karanfilska

Davalieva

2022

Proteomics Clinical Apps

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Purpose: Azoospermia, as the most severe form of male infertility, no longer indicates sterility due to modern medical advancements. The current diagnostic procedure based on testicular biopsy has several drawbacks which urges the development of novel, non-invasive diagnostic procedures based on biomarkers. In the last two decades, there have been many proteomics studies investigating potential azoospermia biomarkers. In this review, we aimed to provide a critical evaluation of these studies.Experimental Design: Published articles were gathered by systematic literature search using Pubmed, Science Direct, and Google Scholar databases until March 2022 and were further preselected to include only studies on human samples.Results: A detailed review of these studies encompassed the proteomics platforms, sources of material, proposed candidate biomarkers, and their potential diagnostic specificity and sensitivity. In addition, emphasis was put on the top, most identified and validated biomarker candidates and their potential for discriminating azoospermia types and subtypes as well as predicting sperm retrieval success rate.Conclusions: Proteomics research of azoospermia has laid the groundwork for the development of a more streamlined biomarker testing. The future research should be focused on well-designed studies including samples from all types/subtypes as well as further testing of the most promising biomarkers identified so far.

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Evaluation of seminal plasma HSPA2 protein as a biomarker of human spermatogenesis status

Cited by 16 publications

References 40 publications

New horizons in human sperm selection for assisted reproduction

New horizons in human sperm selection for assisted reproduction

MicroRNA-targeting in male infertility: Sperm microRNA-19a/b-3p and its spermatogenesis related transcripts content in men with oligoasthenozoospermia

Proteomics of azoospermia: Towards the discovery of reliable markers for non‐invasive diagnosis

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