Evaluation of sensitivity of TaqMan RT-PCR for rubella virus detection in clinical specimens

Okamoto, Kazuhira; Mori, Yoshio; Komagome, Rika; Nagano, Hideki; Miyoshi, Masahiro; Okano, Motohiko; Aoki, Yoko; Ogura, Atsushi; Hotta, Chiemi; Ogawa, Tomoko; Saikusa, Miwako; Kodama, Hiroe; Yasui, Yoshihiro; Minagawa, Hiroko; Kurata, Takako; Kanbayashi, Daiki; Kase, Tetsuo; Murata, Sachiko; Shirabe, Komei; Hamasaki, Mitsuhiro; Kato, Takashi; Otsuki, Noriyuki; Sakata, Masafumi; Komase, Katsuhiro; Takeda, Makoto

doi:10.1016/j.jcv.2016.05.005

Cited by 18 publications

(9 citation statements)

References 6 publications

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“…Rubella virus gene extraction was performed using real-time RT-PCR. TaqMan RT-PCR and nested RT-PCR have been recommended to local public health centers under the guidance of National Institute of Infectious Disease in Japan [16,17]. The TaqMan RT-PCR could detect…”

Section: Laboratory Analysismentioning

confidence: 99%

Conjunctivitis, the key clinical characteristic of adult rubella in Japan during two large outbreaks, 2012–2013 and 2018–2019

et al. 2020

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Background Rubella virus infection mainly causes illness with mild fever, rash, and lymphadenopathy in children; however, the clinical characteristics of adult rubella are not well-known. Methods An observational study was conducted to compare the characteristics between adult rubella and adult non-rubella among participants aged �18 years, with suspected symptomatic rubella. Participants were screened for rubella-specific IgM expression using an enzyme immune assay kit, at a tertiary care hospital in

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Section: Laboratory Analysismentioning

confidence: 99%

Conjunctivitis, the key clinical characteristic of adult rubella in Japan during two large outbreaks, 2012–2013 and 2018–2019

et al. 2020

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“…Throat swab, blood, and urine specimens of rubella‐suspected cases were collected, and the confirmatory laboratory test for rubella was performed by RT‐PCR according to the national guidelines and the previous report at the public health institutes in Sakai, Osaka, and Chiba in accordance with the Act on the Prevention of Infectious Diseases and Medical Care for Patients with Infectious Diseases. Additionally, the following information about the suspected case was collected: gender, age, vaccination history, clinical signs, and the date of rash onset.…”

Section: Summary Of Participants Enrolled In This Studymentioning

confidence: 99%

Clinical value of enzyme immunoassay that detects rubella‐specific immunoglobulin M immediately after disease onset

Kurata

Uchino

Hotta

et al. 2019

Microbiology and Immunology

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A total of 300 patients with nucleic acid test-confirmed rubella, mostly adults, were investigated to determine the clinical value of a rubella-specific IgM test using an EIA kit. IgM titers increased after rash onset, the median IgM titer being significantly higher 3 days post-onset than on previous days (P < 0.0001). Similarly, the IgMpositive rate at 3 days post-onset (61.5%) was significantly higher than on previous days (P < 0.0001). This IgM test against rubella at 3 days or more post-disease onset provides the clinically relevant information. K E Y W O R D S immunoglobulin M, laboratory diagnosis, nucleic acid test, rubella Abbreviations: EIA, enzyme immunoassay; NAT, nucleic acid test; RU, relative unit; WHO, World Health Organization. 32 |

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“…Detection of the rubella virus is performed using molecular methods, especially real-time RT-PCR, for which several well-validated assays are available [60][61][62]. Culture isolation and direct immunofluorescence are no longer in general use, being supplanted by the faster and more sensitive molecular tests.…”

Section: Molecular Detection Of Rubella Virusmentioning

confidence: 99%

Dilemmas and Pitfalls in Rubella Laboratory Diagnostics in Low Prevalence or Elimination Settings

Charlton

Severini

2016

Curr Treat Options Infect Dis

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The changing epidemiology of rubella in populations with either low prevalence levels, or where rubella elimination has been achieved, has challenged laboratory diagnostics. This review will discuss the challenges in identification of acute rubella and outline appropriate confirmatory testing, particularly for cases with low pretest probability. Implementation of pre-analytical screening processes can mitigate follow-up testing required to confirm false positive antibody results, and laboratories should consider not accepting requests for testing that do not have appropriate travel or clinical history. The role of laboratory surveillance will be examined, and challenges in determining protective immunity to rubella infection in light of waning rubella antibody levels in vaccinated populations will be explored. Special populations including prenatal women and the prevention of congenital rubella syndrome will be discussed in the context of low prevalence populations.

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Evaluation of sensitivity of TaqMan RT-PCR for rubella virus detection in clinical specimens

Cited by 18 publications

References 6 publications

Conjunctivitis, the key clinical characteristic of adult rubella in Japan during two large outbreaks, 2012–2013 and 2018–2019

Conjunctivitis, the key clinical characteristic of adult rubella in Japan during two large outbreaks, 2012–2013 and 2018–2019

Clinical value of enzyme immunoassay that detects rubella‐specific immunoglobulin M immediately after disease onset

Dilemmas and Pitfalls in Rubella Laboratory Diagnostics in Low Prevalence or Elimination Settings

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