Evaluation of serologic assays for diagnosis of whooping cough

Granström, Gösta; Wretlind, Bengt; Salenstedt, C. R.; Granström, Marta

doi:10.1128/jcm.26.9.1818-1823.1988

Cited by 58 publications

(15 citation statements)

References 14 publications

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“…The enzyme-linked immunosorbent assay (ELISA), using purified pertussis factors such as pertussis toxin (PT) or filamentous hemagglutinin (FHA), has recently been used extensively for epidemiological studies (8). This technique has proven to be reasonably sensitive and specific (1,3,13). It is generally concluded that a significant increase in specific anti-PT immunoglobulin G (IgG) or IgA and/or anti-FHA IgG or IgA in paired sera correlates with Bordetella pertussis infection.…”

mentioning

confidence: 99%

Evaluation of an Immunoglobulin G Enzyme-Linked Immunosorbent Assay for Pertussis Toxin and Filamentous Hemagglutinin in Diagnosis of Pertussis in Senegal

Simondon¹,

Iteman

Preziosi³

et al. 1998

Clin Diagn Lab Immunol

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The enzyme-linked immunosorbent assay is widely employed for the serological diagnosis of pertussis. It is generally concluded that a significant increase in specific immunoglobulin G (IgG) or IgA against the pertussis toxin (PT) or against filamentous hemagglutinin (FHA) in paired sera correlates with Bordetella pertussis infection. However, this type of diagnosis of pertussis has mainly been applied to unvaccinated children, with timely sampling of acute- and convalescent-phase sera. In current practice and in epidemiological studies, such criteria are not always fulfilled. The aim of this study was to analyze the significance of decreases in IgG antibody titers against PT and FHA between paired sera observed in suspected cases of pertussis infection. Serological results from paired sera were available for 460 children experiencing at least 8 days of cough. An anti-PT IgG decrease was observed in 25% of the children, more frequently than the anti-FHA IgG decrease. Fourteen percent of the serologic decreases were observed in children with culture-confirmed infection, and 59% of the decreases were observed in children with confirmation criteria according to World Health Organization recommendations. Most of the decreases were observed when serum samples were collected according to a standard recommended schedule. Serologic decreases were observed more frequently among vaccinated children than among unvaccinated children. This difference, which was highly significant (P < 0.00001), was explained by the different kinetics of the antibody responses between vaccinated and unvaccinated children. The importance of the antibody response for the evaluation of vaccine efficacy, namely a bias toward higher absolute vaccine efficacy when this response is not taken into account, is discussed. This study supports an earlier recommendation that a significant decrease in PT or FHA should be added to the diagnostic criteria for pertussis.

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confidence: 99%

Evaluation of an Immunoglobulin G Enzyme-Linked Immunosorbent Assay for Pertussis Toxin and Filamentous Hemagglutinin in Diagnosis of Pertussis in Senegal

Simondon¹,

Iteman

Preziosi³

et al. 1998

Clin Diagn Lab Immunol

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“…Procedures currently available for the serodiagnosis of pertussis include the microagglutination test with whole bacteria (7), indirect enzyme-linked immunosorbent assay (indirect ELISA) with purified antigens (2,4,8,10), and Chinese hamster ovary (CHO) cell assay (3). Four pertussis-specific antigens are currently available; these are pertussis toxin (PT), filamentous hemagglutinin (FHA), pertactin, and fimbriae 2 and 3, among which PT is the most important antigen against Bordetella pertussis infection (1,9).…”

mentioning

confidence: 99%

“…Antibody against PT can be measured by ELISA and the CHO cell assay. Among these antibodies, immunoglobulin G (IgG) antibodies against PT detected by ELISA is the most useful for the serodiagnosis of pertussis (2,4). Although ELISA with these purified antigens has been successfully applied to the diagnosis of pertussis, the requirements for special equipment and techniques, as well as difficulties with technical reproducibility, limit its availability (8).…”

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Simple, speedy, sensitive, and specific serodiagnosis of pertussis by using a particle agglutination test

et al. 1997

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We developed a particle agglutination test (KPA) with poly(␥-methyl L-glutamate) as the solid particle for measurement of pertussis toxin (PT) antibody. In this study, KPA was assessed as a means of serodiagnosing pertussis, and the results were compared with those of indirect enzyme-linked immunosorbent assay (indirect ELISA) and the microagglutination test. First, four serum samples were collected from each of 21 healthy children: before and 4 weeks after receiving three primary doses of acellular pertussis vaccines and before and 4 weeks after receiving a booster dose. In all 21 vaccinees, a significant rise in PT antibody titers was observed by KPA after each vaccination, and among all 84 serum samples collected, an excellent correlation was demonstrated between the values obtained by indirect ELISA and those obtained by KPA (r ‫؍‬ 0.92). Second, paired serum samples were collected at intervals of approximately 2 weeks from 51 patients with cultureconfirmed pertussis. A significant increase in titer (fourfold or more) was observed in 39 (76%) patients by KPA, 34 (67%) patients by indirect ELISA, and 23 (45%) patients by the microagglutination test. In acute-and convalescent-phase sera collected from 20 nonpertussis patients, there were no changes in titers by KPA. The KPA procedure was as simple as that of the microagglutination test, and the reaction time was only 2 h (or overnight). In this study, KPA was demonstrated to be a simple, speedy, sensitive, and specific serodiagnostic method for pertussis.

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“…Purified FHA and PT have been under intensive investigation for use as immunogens in experimental acellular pertussis vaccines (1, 14) and as antigens for the serodiagnosis of pertussis (2,4,(18)(19)(20). Because of meager antigen yields in the commonly used growth media (3,16) and the relatively complicated purification techniques, these antigens have been quite expensive and hard to obtain.…”

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confidence: 99%

Simple, efficient purification of filamentous hemagglutinin and pertussis toxin from Bordetella pertussis by hydrophobic and affinity interaction

Skelton

Wong

1990

J Clin Microbiol

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Two major antigens of Bordetella pertussis, filamentous hemagglutinin (FHA) and pertussis toxin (PT), were efficiently purified from culture filtrate by exploiting their relative hydrophobicities and differences in affinity to sialic acid-containing protein. High yields of FHA (40 to 80 mg/liter) and PT (8 to 16 mg/liter) were first produced by growing the bacteria in the modified CL medium. The FHA and PT in the culture filtrate were adsorbed onto butyl-Sepharose by hydrophobic interaction at appropriately high ionic strength. Elution of the antigens was effected by decreasing their hydrophobicities with a buffer of low ionic strength. FHA was then separated from PT with an affinity column of fetuin-Sepharose. The fraction passing through the column contained purified FHA, and the fetuin-bound PT was eluted with buffered MgCl2. The FHA and PT purified by these steps were electrophoretically and serologically identical to the reference purified FHA and PT preparations. Approximately 16 to 32 mg of purified FHA and 4 to 8 mg of purified PT were obtained from 1 liter of culture filtrate. The described procedure for making FHA and PT antigens from B. pertussis for serologic and immunologic use is very simple, efficient, and reproducible.

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Evaluation of serologic assays for diagnosis of whooping cough

Cited by 58 publications

References 14 publications

Evaluation of an Immunoglobulin G Enzyme-Linked Immunosorbent Assay for Pertussis Toxin and Filamentous Hemagglutinin in Diagnosis of Pertussis in Senegal

Evaluation of an Immunoglobulin G Enzyme-Linked Immunosorbent Assay for Pertussis Toxin and Filamentous Hemagglutinin in Diagnosis of Pertussis in Senegal

Simple, speedy, sensitive, and specific serodiagnosis of pertussis by using a particle agglutination test

Simple, efficient purification of filamentous hemagglutinin and pertussis toxin from Bordetella pertussis by hydrophobic and affinity interaction

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