Evaluation of Somaclonal and Ethyl Methane Sulfonate-Induced Genetic Variation of Mexican Oregano (Lippia graveolens H.B.K.)

Abstract

Lippia graveolens, commonly known as Mexican oregano, is an aromatic plant of great industrial, nutritional, and medicinal value, principally for its essential oils. Regeneration via axillary buds was established in MS medium supplemented with 6-benzyladenine (BA) (0.5 mgL−1) as a growth regulator. Three genotypes and three stages of cultivation were considered in the study. On average, 3.5, 4.2, and 6.4 shoots induced per explant were obtained for genotypes B, C, and D, respectively. Several doses (0.1, 0.3, and 0.5%) of ethyl methane sulfonate (EMS) and different exposure times (1, 2, and 3 h) were applied to investigate the effect of the chemical mutagen on the formation of axillary buds. Genetic variation among the collected plants, the micro-propagated plants during three sub-cultivations, and the plants regenerated in the presence of the mutagen was evaluated by means of randomly amplified microsatellite polymorphism (RAMP) markers. A high genetic stability was observed in the micro-propagation of Mexican oregano for the three genotypes and three sub-cultivations, presenting 100% of monomorphic bands. The genetic variation observed in the different collections of wild populations (A, R, and V) and after treatment with EMS regarded 34 and 35% of polymorphic loci, respectively.

Desarrollo de un método eficiente para la micropropagación de orégano

“…Sin embargo, el número de brotes obtenidos con la hormona 6-BAP fue mayor a los resultados reportados en otras investigaciones de esta especie. Muñoz-Miranda et al (2019) reporta un máximo de 6.4 brotes con BA a una concentración de 0.5 mg L -1 , mientras Castellanos-Hernández et al (2013) menciona 2.8 brotes por explante con BA a esa misma concentración.…”

Effect of Bead Composition, PVS Type, and Recovery Medium in Cryopreservation of Bleeding Heart ‘Valentine’—Preliminary Study

“…Moreover, offspring do not have the same genetic set up when propagated by seed. In-vitro, slow-growth conservation is possible [9], but it comes with a cost and the possibility that somaclonal variation will occur over time [10]. Cryopreservation can solve these issues and allows us to store plant genetic resources at the ultra-low temperature of liquid nitrogen (LN), at which biological and chemical activities are halted.…”

Impacto del manejo in situ-ex situ del orégano mexicano ( Lippia origanoides Kunth) en el noroeste de Yucatán

“…Existen numerosos estudios experimentales sobre el manejo agronómico (Dunford & Silva-Vázquez 2005, Silva et al 2015, Martínez-Hernández et al 2017) y biotecnológico (Muñoz-Miranda et al 2019, Aarland et al 2020, Sánchez-Velázquez et al 2021) de la especie. Sin embargo, son escasos y limitados los estudios del manejo tradicional en agroecosistemas.…”