Evaluation of synthetic serum substitute versus serum as protein supplementation for mouse and human embryo culture

Tucker, Kathleen; Hurst, Bradley S.; Guadagnoli, Susie; Dymecki, Cecelia; Mendelsberg, Barbara; Awoniyi, Caleb A.; Schlaff, William D.

doi:10.1007/bf02068866

Cited by 20 publications

(16 citation statements)

References 9 publications

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“…Desai et al [3] reported that Synthetic Serum Substitute 1 with alphaMEM medium may be a good basal medium for culture of human embryos to the blastocyst stage. Tucker et al [35] demonstrated that Synthetic Serum Substitute 1 is a good protein source for the culture of mouse embryos.…”

Section: Discussionmentioning

confidence: 99%

Use of two replacements of serum during bovine embryo culture in vitro

et al. 2003

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This study evaluated the effect of two commercial serum replacements (Ultroser G 1 and CPSR-3 1 ) on in vitro bovine embryo culture. In Experiment 1, zygotes were cultured in SOF Ultroser G 1 (2, 4 and 6%), SOF CPSR-3 1 (2, 4 and 6%), and SOF 5% FCS (control). Blastocyst rates obtained after culturing with Ultroser G 1 were lower than those with FCS. However, blastocyst rates for CPSR-3 1 were similar to those for serum. In addition, embryos produced in SOF CPSR-3 1 had the same proportion inner cell mass number and total cell number as embryos cultured with FCS. In Experiment 2, a combination of serum replacements during different periods showed that treatment before the ®ve-to eight-cell stages had no effect on further embryo development. However, treatments up to the morula stage affected blastocyst formation. The concentration of supplement and the timing of its inclusion in culture markedly affected embryo development. The serum replacement CPSR-3 1 can supplement embryo culture with blastocyst rates and quality similar to those for serum. #

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Section: Discussionmentioning

confidence: 99%

Use of two replacements of serum during bovine embryo culture in vitro

et al. 2003

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“…Since its introduction for use in IVF laboratories, the mouse embryo assay (MEA) has been instrumental for quality control (QC) and quality improvement by establishing basic guidelines for improving human embryo growth in vitro (1)(2)(3)(4). The MEA tests mainly the in vitro developmental potential of in vivo obtained mouse zygote or twocell embryos to the blastocyst stage.…”

mentioning

confidence: 99%

Double-quality control reveals high-level toxicity in gloves used for operator protection in assisted reproductive technology

Lierman

Sutter

Dhont

et al. 2007

Fertility and Sterility

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“…The first difference is the presence of alpha- and beta-globulin in MRC media. Several studies have reported that the use of synthetic serum substitute containing alpha- and beta-globulin has beneficial effects during the culture of human and mouse embryos [18,20,30]. The second difference is the use of hyaluronic acid in MRC media, which is found at high concentrations in the fluid of the female reproductive tract [31-33].…”

Section: Discussionmentioning

confidence: 99%

Application of two different synthetic sequential media for the human IVF-ET program: a prospective, randomized, and comparative study

Yoon

Juhn

et al. 2011

Clin Exp Reprod Med

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ObjectiveSince IVF program was first established, various types of media and culture systems have been developed either in-house or commercially. The aim of this study was to compare the efficacy of in-house Maria Research Center (MRC) media to that of commercially available Sydney IVF media in human day 3 embryo transfer cycles.MethodsThree hundred sixty nine couples were included in this prospective, randomized, and comparative study. All couples undergoing IVF treatment at the Maria Fertility Hospital were randomly assigned to either Sydney IVF (n=178) or MRC (n=191) media.ResultsNo difference was observed between the MRC media and Sydney IVF media groups with respect to fertilization rate (74.4% vs. 75.5%). The clinical pregnancy and implantation rates of MRC media (47.1% and 20.0%, respectively) were also similar to those of Sydney IVF media (44.4% and 19.4%, respectively). However, the proportion of embryos with good quality on day 3 was significantly higher in the MRC media group than the Sydney IVF media group (50.2% vs. 43.2%) (p<0.05).ConclusionMRC media were as effective as Sydney IVF media for sustaining embryo development and pregnancy rates. The present study implies that MRC media can be a suitable alternative to commercially available media for human IVF-ET program.

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Evaluation of synthetic serum substitute versus serum as protein supplementation for mouse and human embryo culture

Abstract: SSS appears to be a superior protein source for mouse embryo growth and is as good as serum from fertile donors in promoting in vitro human embryo development.

Cited by 20 publications

References 9 publications

Use of two replacements of serum during bovine embryo culture in vitro

Use of two replacements of serum during bovine embryo culture in vitro

Double-quality control reveals high-level toxicity in gloves used for operator protection in assisted reproductive technology

Application of two different synthetic sequential media for the human IVF-ET program: a prospective, randomized, and comparative study

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