Evaluation of the Autof MS1000 mass spectrometer in the identification of clinical isolates

Abstract

Background To evaluate the accuracy and performance of the Autof MS1000 mass spectrometer in bacteria and yeast identification, 2342 isolates were obtained from microbial cultures of clinical specimens (e.g. blood, cerebrospinal fluid, respiratory tract samples, lumbar puncture fluid, wound samples, stool, and urine) collected in 2019 in Henan Provincial People’s Hospital. Repetitive strains from the same patient were excluded. We tested the Autof MS1000 and Bruker Biotyper mass spectrometry systems and the classical biochemical identification system VITEK 2/API 20C AUX. Inconsistencies in strain identification among the three systems were identified by 16S rDNA and gene sequencing. Results At the species level, the Autof MS1000 and Bruker Biotyper systems had isolate identification accuracies of 98.9 and 98.5%, respectively. At the genus level, the Autof MS1000 and Bruker Biotyper systems were 99.7 and 99.4% accurate, respectively. The instruments did not significantly differ in identification accuracy at either taxonomic level. The frequencies of unreliable identification were 1.1% (26/2342) for the Autof MS1000 and 1.5% (34/2342) for the Bruker Biotyper. In vitro experiments demonstrated that the coincidence rate of the Autof MS1000 mass spectrometer in the identification of five types of bacteria was > 93%, the identification error rate was < 3%, and the no identification rate was 0. This indicates that the Autof MS1000 system is acceptable for identification. Conclusions The Autof MS1000 mass spectrometer can be utilised to identify clinical isolates. However, an upgradation of the database is recommended to correctly identify rare strains.

Evaluation of Autof MS2600 and MBT Smart MALDI-TOF MS Systems for Routine Identification of Clinical Bacteria and Yeasts

“…To this regard, the Autobio MS1000 mass spectrometer from Autobio Diagnostics has been recently presented as the first system in the landscape of the identification of clinical pathogenic microorganisms. The instrument is equipped with an ion source vacuum (up to 10 −7 mPa) and possesses higher speed over the existing systems being able to deliver a full target plate (96 tests) in about 20 min; moreover, its performance of identification has been proved comparable to the commonly used MALDI-TOF systems [ 10 ].…”

“…Until now, only three papers have been published that referred to the Autobio MS1000 platform performance of identification [ 10 , 11 , 12 , 13 ]; nonetheless, to the best of our knowledge, there does not exist any research in the literature regarding the evaluation of the Autobio MS2600 mass spectrometer for the routine identification of clinical microorganisms.…”

Evaluation of the Autof ms1000 mass spectrometry for rapid clinical identification of filamentous fungi

“…Since 2018, the Autof ms1000 has been successfully used in microbiological laboratories for routine bacteria identification [ 10 , 11 ]. However, its use for identifying filamentous fungi has been limited.…”

Evaluation of the Zybio EXS3000 mass spectrometry in routine identification of Clinical isolates

“…At present, the most widely used MS-based microbial identification systems worldwide are the BioTyper (Bruker Daltonics GmbII, Germany) and VITEK MS (bioMérieux, France) systems [ 11 ]. Other more recently introduced MALDI-TOF MS devices based on similar principles, such as MicroIDSys system (ASTA corp., South Korea), Clin-TOF (Bioyong Technologies, China), and Autof ms1000 (Autobio Diagnostics, China) are also gradually put into use in clinical laboratories [ [12] , [13] , [14] ].…”