Evaluation of the chondrogenic differentiation of mesenchymal stem cells on hybrid biomimetic scaffolds

Karkhaneh, Akbar; Naghizadeh, Ziba; Shokrgozar, Mohammad Ali; Bonakdar, Shahin

doi:10.1002/app.40635

Cited by 17 publications

(16 citation statements)

References 34 publications

(44 reference statements)

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“…The 5AD‐5CMC hydrogel created a more closely packed structure. Thus, these results are comparable to the native cartilage modulus and other studies in this field …”

Section: Resultssupporting

confidence: 90%

“…Weight loss was monitored over time at 37°C. The percentage of weight loss at any time (Δ W %) was calculated by the following formula:

Δ W true(% true) = true(W_{0} - W_{normalt} true) / W_{0} \times 100 true(% true)

…”

Section: Methodsmentioning

confidence: 99%

“…A working range of the standard solution of a known concentration of chondroitin sulfate C (shark cartilage extract, Sigma) was used. The GAG content was quantified using ELISA plate reader at 530 nm …”

Section: Methodsmentioning

confidence: 99%

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Simultaneous release of melatonin and methylprednisolone from an injectable in situ self‐crosslinked hydrogel/microparticle system for cartilage tissue engineering

Naghizadeh

Karkhaneh

Khojasteh

2018

J Biomedical Materials Res

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Recently, injectable hydrogel/microparticle systems have so considered for tissue engineering and regenerative medicine. In this study, we produced an injectable in situ self-crosslinked hydrogel/microparticle system for simultaneous dual drug delivery. First, melatonin conjugated chitosan microparticle loaded with methylprednisolone (MCC-MP) microparticle was fabricated by the covalent linkage of melatonin to chitosan by N-hydroxysuccinimide (NHS)1-Ethyl-3-(3-dimethylamino propyl)-carbodiimide (EDC) followed by an ionic gelation of MCC and MP using tripolyphosphate (TPP). Second, the hydrogel was prepared by the connection between the aldehyde group of alginate oxide (AD) and the amine group belonging to carboxymethyl chitosan (CMC) via Schiff base reaction. Finally, microparticle was incorporated into the AD-CMC hydrogel to produce a hydrogel/microparticle system. Hydrogel/microparticle was assessed by many techniques including microscopy, spectroscopy, particle size measurements, mechanical analysis, injectability, rheological analyses to ascertain hydrogel/microparticle properties. The biological assays of mesenchymal stem cells (MSCs) culture, 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), acridine orange/propidium iodide (AO/PI), and 4, 6-diamidino-2-phenylindole (DAPI) to assess cell viability and dimethylmethylene blue (DMMB) to evaluate proteoglycan content were done. The release profiles of melatonin and MP showed acceptable release after 60 and 20 days, respectively. The hydrogel/microparticle system has the ability to sustain cells alive. A higher rate of proteoglycan content was observed in hydrogel/microparticle as compared with hydrogel. With appropriate biocompatibility and adequate properties, this system can be a proper alternative for cartilage tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1932-1940, 2018.

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“…The 5AD‐5CMC hydrogel created a more closely packed structure. Thus, these results are comparable to the native cartilage modulus and other studies in this field …”

Section: Resultssupporting

confidence: 90%

“…Weight loss was monitored over time at 37°C. The percentage of weight loss at any time (Δ W %) was calculated by the following formula:

Δ W true(% true) = true(W_{0} - W_{normalt} true) / W_{0} \times 100 true(% true)

…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous release of melatonin and methylprednisolone from an injectable in situ self‐crosslinked hydrogel/microparticle system for cartilage tissue engineering

Naghizadeh

Karkhaneh

Khojasteh

2018

J Biomedical Materials Res

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“…For the reference purpose, cells were seeded into a fresh culture medium (control) with same seeding condition. The percentage (%) viability was estimated as per the following formula:

Viability % = ({OD}_{s} / {OD}_{c}) \times 100

where OD s and OD c are the average optical densities of the sample and the control, respectively . Each assay was performed four times.…”

Section: Methodsmentioning

confidence: 99%

Preparation and characterization of (PCL‐crosslinked‐PEG)/hydroxyapatite as bone tissue engineering scaffolds

Koupaei

Karkhaneh

Joupari

2015

J Biomedical Materials Res

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In this study, interconnected porous bioactive scaffolds were synthesized for bone tissue engineering. At the first step, poly( ɛ-caprolactone) (PCL) diols were diacrylated with acryloyl chloride. Then, the scaffolds were synthesized by radical crosslinking reaction of PCL and poly(ethyleneglycol) (PEG) diacrylates in the presence of hydroxyapatite (HA) particles. Morphological, swelling, thermal, and mechanical characteristics as well as degradability of the scaffolds were investigated. Results showed that increasing the ratio of PEG to PCL led to significant increase of swelling ratio and degradation rate, and decrease of crystallinity and compressive modulus of the networks, respectively. It was found that the incorporation of HA particles with the polymer matrices resulted in an augmented crystallinity, a decreased swelling ratio, and also a significantly increased compressive modulus of the networks. Cytocompatability and osteoconductivity of the scaffolds were assessed by MTT and alkaline phosphatase (ALP) assays, respectively. The results confirmed the cytocompatible nature of PCL/PEG/HA scaffolds with no toxicity. MG-63 cells attached and spread on the pore walls offered by the scaffolds. PCL/PEG/HA scaffolds compared with PCL/PEG ones showed higher ALP activity. Thus, the results indicated that the PCL/PEG/HA scaffolds have the potential of being used as promising substrates in bone tissue engineering.

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“…[160][161][162][163][164] However, there appears to have some limitations in achieving identical properties of native cartilages. Additionally, generation of functional cartilage by MSCs has been found to be troublesome, as it depends on the viable cell source for extracellular cartilage matrix production, leading to high quality cartilage regeneration.…”

Section: Cartilagementioning

confidence: 99%

Fabrication of polymeric biomaterials: a strategy for tissue engineering and medical devices

2015

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Polymeric biomaterials have significant impact in today's health care technology. Polymer hydrogels were the first experimentally designed biomaterials for human use. In this article the design, synthesis and properties of hydrogels, derived from synthetic and natural polymers and their use as biomaterials in tissue engineering are reviewed. The stimuliresponsive hydrogels with controlled degradability and examples of suitable methods for designing such biomaterials, using multidisciplinary approaches from traditional polymer chemistry, materials engineering to molecular biology, have been discussed. Examples of the fabrication of polymer-based biomaterials, utilized for various cells type manipulations for tissue re-generation are also elaborated. Since a highly porous three-dimensional scaffold is crucially important in cellular process, for tissue engineering, recent advances in effective methods of scaffolds fabrication are described. Additionally, the incorporation of factor molecules for the enhancement of tissue formation and their controlled release are also elucidated in this article. Finally, the future challenges in the efficient fabrication of effective polymeric biomaterials in tissue regeneration and medical devices applications.

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Evaluation of the chondrogenic differentiation of mesenchymal stem cells on hybrid biomimetic scaffolds

Cited by 17 publications

References 34 publications

Simultaneous release of melatonin and methylprednisolone from an injectable in situ self‐crosslinked hydrogel/microparticle system for cartilage tissue engineering

Simultaneous release of melatonin and methylprednisolone from an injectable in situ self‐crosslinked hydrogel/microparticle system for cartilage tissue engineering

Preparation and characterization of (PCL‐crosslinked‐PEG)/hydroxyapatite as bone tissue engineering scaffolds

Fabrication of polymeric biomaterials: a strategy for tissue engineering and medical devices

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