Evaluation of the currently used polymerase chain reaction assays for molecular detection of Brucella species

Abstract

The diagnosis of brucellosis is the corner stone in any control and eradication program. Therefore, the main objective of the present study was to apply more advanced techniques for rapid and accurate diagnosis of brucellosis that can overcome the draw backs of the traditional diagnostic techniques. Different polymerase chain reaction (PCR) assays were applied in the present study, either singly or in a multiplex format, that enable to detect and differentiate most of Brucella species. The PCR assay detection limit was evaluated in a preliminary study. The obtained results recommend the PCR assay as a valuable, rapid, very specific, highly sensitive and safe laboratory diagnostic test that can be used not only for detection of Brucella antigen either in culture or in clinical samples but also in differentiating most of the virulent and vaccinal strains.

Molecular characterization of Brucella species in cattle, sheep and goats obtained from selected municipalities in the Eastern Cape, South Africa

“…Serological tests have proved to be either too sensitive, giving false-positive results, or too specific, giving false-negative results [5][6][7]. The presence of antibodies does not always mean an active case of brucellosis, as vaccinated animals tend to yield persistent post-vaccinal immune responses and other Gramnegative bacteria such as Yersinia enterocolitica cross-react with Brucella spp.…”

Single-tube duplex-PCR for specific detection and differentiation of Brucella abortus S19 vaccine strain from other Brucella spp

“…Multiplex PCR for differential identification of Brucella species have been reported (Moussa et al, 2011). AMOS PCR, one of the most popular PCR assays for the differentiation of Brucella species is based on restriction fragment length polymorphism (RFLP), which requires numerous steps, laborious and time-consuming (Tabit, 2016).…”

Molecular Identification of Brucella abortus Collected from Whole Blood Samples of Seronegative Dairy Cattle with Reproductive Disorders in Central Java, Indonesia

“…The antibody level and the number of the bacteria in the circulation were affected by the infection status of the animals at the time of specimen collection (Moussa et al, 2011;Raghava et al, 2017). Brucella is a facultative intracellular microorganism.…”