Evaluation of the Effect of Two Systemic Doses of HESA-A on Prevention of Induced Tongue Neoplasm in Rats

Mehdipour, Masoumeh; Zenouz, Ali Taghavi; Abbasi, Mehran Mesgari; Mohajeri, Daryoush; Damghani, Hossein; Helli, Sanaz; Abdollahi, Bita

doi:10.5681/joddd.2013.035

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…On the other hand, Makita et al described weight gain in the 4-NQO group compared to its experimental groups. Water and feed consumption was described by some authors as not affected by exposure to 4NQO, however, other studies described results similar to ours (Shuvy et al 2011;Mehdipour et al 2013).…”

Section: Discussionsupporting

confidence: 84%

“…The other groups demonstrated weight gain. Mehdipour et al (Mehdipour et al 2013) show loss of body weight in all 4-NQO studied groups. Araki and Salvadori (Ribeiro and Salvadori 2007) described an expressive reduction of body weight in animals exposed to 4-NQO.…”

Section: Discussionmentioning

confidence: 82%

“…It represented a decrease of 52.4% by group and 71.3% in the cancer/rat ratio. Other studies also showed better activity in lower doses in natural chemotherapy treatment, including modulation in epithelial dysplasia and, specifically, moderate dysplasia (Ribeiro and Salvadori 2007;Helli et al 2016) , (Renugadevi and Prabu 2010;Santos-Nascimento et al 2015;Baracho et al 2016) , (Mehdipour et al 2013) and other researchers observed better results in the highest dose, including epithelial acantosis and epithelial dysplasia (Mehdipour et al 2013) , (Yoshida et al 2005). In addition, reduction of squamous cell carcinoma in situ, microinvasive and invasive have also been described.…”

Section: Discussionmentioning

confidence: 89%

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Negative terpinen-4-ol modulate potentially malignant and malignant lingual lesions induced by 4-nitroquinoline-1-oxide in rat model

Neto

Sorbo

Filho

et al. 2022

Naunyn-Schmiedeberg's Arch Pharmacol

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Our aim was to verify the modulative TP-4-ol capacity in 4-nitroquinoline-1-oxide induced oral rat cancer. Methods: The stereoisomers of TP-4-ol were used against the human tongue squamous cell line and the negative stereoisomer showed lower IC50. 31 Holtzman rats (120-130 grams) were cancer induced by 4-nitroquinoline-1-oxide (4-NQO/8 weeks/25 ppm) and 32 Holtzman rats (120-130 grams) were used to Healthy and TP-4-ol toxicity experiments. Six groups were used, healthy, 0.1nL/g of TP-4-ol, 8nL/g of TP-4-ol, 4-NQO, 4-NQO+0.1nL/g of TP-4-ol and 4-NQO+8nL/g of TP-4-ol. We performed the toxicity analysis by biochemical and histopathological analysis. The biochemistry analysis includes alkaline phosphatase [ALP], alanine aminotransferase [ALT], aspartate transaminase [AST], Urea and Creatinine and the histopathology analysis includes the liver, kidney, lung, and spleen. Specifically, for malign modulation we performed a macroscopic and microscopic analysis. Results: The group exposed to 0.1nL/g of TP-4-ol demonstrated a reduced risk of malignancy in dysplasia considering the criteria of architecture and cytology. Similarly, a drop of percentual rats with SCC diagnosis was observed in 4-NQO+0.1nL/g (41.6%) when compared to 4-NQO (87.5%). Moreover, the 4-NQO group presented a median of 2.62 SCC/rat and the 4-NQO+0.1nL/g demonstrated a median of 0.75 SCC/rat. For toxicity analysis, 4-NQO+0.1nL/g showed focal necrosis in the kidney and 4-NQO showed lung hemorrhagic areas. Conclusion: The concentration of 0.1nL/g was more effective in reducing the tongue induction of potentially malignant and malignant lesions by 4-NQO. A kidney toxicity was observed in healthy animals exposed to 0.1nL/g of TP-4-ol. Clinical relevance:The negative isoform of Terpinen-4-ol negatively modulates the development of potentially malignant and malignant lesions in rats (Rattus nonverdicts albinos, Holtzman) exposed to 4-NQO. (-)-Terpinen-4-ol reduced the mice percentual with squamous cell carcinoma, 87.5% to 41.6% and decreased the cancer/rat ratio of 2.62 in 4-NQO to 0.75 in 4-NQO+0.1nL/g. This represents 52.4% by group and 71.3% in the cancer/rat ratio.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

Negative terpinen-4-ol modulate potentially malignant and malignant lingual lesions induced by 4-nitroquinoline-1-oxide in rat model

Neto

Sorbo

Filho

et al. 2022

Naunyn-Schmiedeberg's Arch Pharmacol

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Protection from ionizing radiation-induced genotoxicity and apoptosis in rat bone marrow cells by HESA-A: a new herbal-marine compound

Hazbavi

Zarei

Nazaralivand

et al. 2019

J Bioenerg Biomembr

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Hesa-A Effects on Cell Cycle Signaling in Esophageal Carcinoma Cell Line

Ahmadian

Pashaei-Asl

Samadi

et al. 2016

Middle East J Dig Dis

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BACKGROUNDHesa-A is a natural compound with anticancer properties. The exact mechanism of its action in esophageal cancer is not clear, yet. The aim of this study was to evaluate the cell toxicity effect of Hesa-A on the esophageal carcinoma cell lines, KYSE-30, and cell cycle genes expression.METHODSIn this study, we tested cell toxicity with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay and flow cytometry to evaluatet he cell cycle arrest. Real time polymerase chain reaction was used to assess the expression of P53, P16, P21, cyclin D1, and cyclin B1 genes.RESULTSOur results showed that Hesa-A is effective in the expression of cell cycling check point proteins. Hesa-A induced an arrest in G2 phase of esophageal cell cycle. The levels of P53 (>13 times), P21 (>21 times), P16, cyclin B1, and cyclin D1 genes were increased 48 hours after Hesa-A treatment.CONCLUSIONP21 and P16 expression were the potential mechanisms for G2 arrest of KYSE-30 esophageal cancer cell line by Hesa-A.

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Evaluation of the Effect of Two Systemic Doses of HESA-A on Prevention of Induced Tongue Neoplasm in Rats

Cited by 7 publications

References 16 publications

Negative terpinen-4-ol modulate potentially malignant and malignant lingual lesions induced by 4-nitroquinoline-1-oxide in rat model

Negative terpinen-4-ol modulate potentially malignant and malignant lingual lesions induced by 4-nitroquinoline-1-oxide in rat model

Protection from ionizing radiation-induced genotoxicity and apoptosis in rat bone marrow cells by HESA-A: a new herbal-marine compound

Hesa-A Effects on Cell Cycle Signaling in Esophageal Carcinoma Cell Line

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