Evaluation of the Effects of Pediococcus acidilactici Isolated from Wara, a Nigerian Milk Product, in the Prevention of Diarrhea and the Modulation of Intestinal Microflora in Wistar Rats

Abstract

Animals have been used in the evaluation of probiotic potentials of lactic acid bacteria for the development of functional food as only in-vitro tests may not be enough to ascertain the probiotic ability of an organism and its safety in a living host. The action of a probiotic Pediococcus acidilactici strain isolated from Wara, a Nigerian milk product, in the treatment of Diarrhea in wistar rats infected orally with Diarrhoeagenic Enterotoxigenic Escherichia coli as well as the survival and microflora modulation of the probiotic strain in the gastrointestinal tract were evaluated. Five groups of seven rats each were infected and treated as the case may be with the pathogen and probiotic respectively. Each group received specific treatments for 30 days, during which the animals were closely monitored and the faecal samples were analyzed for the trend of E. coli and LAB counts. Also, specific organs of the Gastrointestinal tract (GIT) were examined for any histomorphological disparity after 30 days of daily treatment. Clinical signs were observed in the pathogen challenged animals while the probiotic treated groups displayed less pronounced Diarrheal symptoms and a negligible E. coli count. The strain adhered to the mucosal wall and did not initiate any adverse effect on the organs of the GIT after treatment. It was concluded that Pediococcus acidilactici isolated from Wara, a Nigerian milk product, was able to improve gut health through pathogen exclusion and had no adverse effect on the general health of the host.

Isolation, identification, and fermentation characteristics of endogenous lactic acid bacteria derived from edible mushrooms

“…If used in fermented meat products, it can significantly improve the texture and flavor of meat products (Jiang et al, 2023). It can also regulate intestinal flora and improve the immunity (Olajugbagbe et al, 2020). Moreover, P. pentosaceus can control foodborne pathogens, regulate intestinal microflora, reduce cholesterol, and so on.…”

Effect of Some Strains of Lactic Acid Bacteria Isolated From Some Fermented Dairy Products on Carbon Tetrachloride-Induced Hepatotoxicity and Nephrotoxicity of Albino Rats.

“…SBT 2028 had the best antioxidant effects out of 570 strains of LAB (Kaizu et al, 1993) . 19 strains of LAB have antioxidant of 7-12 percent in intracellular cell-free extracts, due to their metal-ionchelating and ROS-scavenging capabilities (Lin and Yen, 1999), Olajugbagbe et al(2020) indicated that Pediococcus acidilactici isolated from Wara was able to act as a therapeutic agent against a Diarrhoeagenic Enterotoxigenic E. coli and improve gut health . Several scienti c studies have con rmed that the daily ingestion of probiotics or their related products, notably lactic acid bacteria, signi cantly improved human health through a variety of effects, including xenobiotic detoxi cation and competition with pathogenic microbial agents (Maurice et al, 2013), Silymarin is a common herbal extract used as a hepatoprotective agent a mixture of avonolignans obtained from milk thistle (Williamson et al, 1996) , In natural dietary sources, antioxidants are available, and the intake of antioxidants have a number of possible health bene ts (Yeung et al, 2019) .…”