Evaluation of the Effects of Homologous Platelet Gel on Healing Lower Extremity Wounds in Patients With Diabetes

Abstract

The treatment of chronic diabetic wounds remains complicated, despite new insight into the cellular and molecular basis of wound healing and cutaneous regeneration. A growing body of clinical trials has shown that platelet release has a notable effectiveness on refractory ulcer healing. However, patients with chronic diabetic ulcers usually have poor general health, and the large-volume blood absence required to produce autologous platelet-rich plasma often causes adverse effects. To overcome the limitation, the homologous platelet gel (PG) from healthy donor was used for the treatment of chronic diabetic lower extremity wound in the study. We show here that homologous derived platelets significantly enhanced EVC304 cell and HaCaT cell proliferation and homologous PG was capable of prompting cell migration. Twenty-one patients with refractory diabetic lower extremity ulcers, who had no response to conventional treatments, were treated in this study. Our data indicated that homologous PG was effective for the enhancement and acceleration of diabetic lower extremity wounds healing. We propose that homologous PG appeared to enhance vascularization and epithelialization, which might induce a quicker healing process and and encourage controlled studies in future.

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

“…In this study, where we focused on wounds not associated with compromised blood and located in regions with good blood circulation, heterologous PRP may not induce faster wound reduction or alterations in tissue contraction during the repair process, similar to the results by Bauer et al 27 In contrast, some studies that presented superior effects of PRP studied chronic wounds in peripheral regions or in patients with some type of circulatory compromise 11,12,24,28 .…”

“…Despite the proven efficacy of autologous PRP in the aforementioned studies, another type of safe treatment can be useful when the patient's general condition prevents the use of his/her own blood for the production of this concentrate 11 .…”

Effect of autologous platelet-rich plasma in the promotion of healing of chronic ulcers

“…21 Gui-Qiu Shan et al, in 2013 studied the effect of PRP on the healing of lower extremity chronic ulcers in which 21 patients with chronic ulcers are included in the study they concluded that, there no significance between type and site of ulcers in correlation with the rate of healing. 22 This supports the results we found in our study.…”

Platelet Derivatives in Regenerative Medicine: An Update

“…Of course, many ongoing studies suggest great interest of the scientific community on this topic (Tables 5 and 6) [7,[89][90][91][92][93][94][95][96][97].…”