Evaluation of the <i>in vitro</i> cytotoxicity of the antimicrobial peptide P34

Vaucher, Rodrigo de Almeida; Motta, Amanda de Souza da; Brandelli, Adriano

doi:10.1042/cbi20090025

Cited by 54 publications

(26 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Briefly, cells were incubated with serial dilutions (ranging from 0.31 to 100 g/ml) of either eCATH1, DEFA1, nisin, or 1% (vol/vol) Triton X-100 (positive control) for 24 h. Nisin was used here as a "safe control" because of its generally recognized as safe (GRAS) status in the food industry. The percentage of cytotoxicity was calculated as described by Vaucher et al (43). Each experiment was performed in triplicate, and values were expressed as mean Ϯ standard error.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Potential of Equine DEFA1 and eCATH1 as Alternative Antimicrobial Drugs in Rhodococcosis Treatment

Schlusselhuber¹,

Jung

Bruhn³

et al. 2012

Antimicrob Agents Chemother

View full text Add to dashboard Cite

f Rhodococcus equi, the causal agent of rhodococcosis, is a severe pathogen of foals but also of immunodeficient humans, causing bronchopneumonia. The pathogen is often found together with Klebsiella pneumoniae or Streptococcus zooepidemicus in foals. Of great concern is the fact that some R. equi strains are already resistant to commonly used antibiotics. In the present study, we evaluated the in vitro potential of two equine antimicrobial peptides (AMPs), eCATH1 and DEFA1, as new drugs against R. equi and its associated pathogens. The peptides led to growth inhibition and death of R. equi and S. zooepidemicus at low micromolar concentrations. Moreover, eCATH1 was able to inhibit growth of K. pneumoniae. Both peptides caused rapid disruption of the R. equi membrane, leading to cell lysis. Interestingly, eCATH1 had a synergic effect together with rifampin. Furthermore, eCATH1 was not cytotoxic against mammalian cells at bacteriolytic concentrations and maintained its high killing activity even at physiological salt concentrations. Our data suggest that equine AMPs, especially eCATH1, may be promising candidates for alternative drugs to control R. equi in mono-and coinfections.

show abstract

Section: Methodsmentioning

confidence: 99%

In Vitro Potential of Equine DEFA1 and eCATH1 as Alternative Antimicrobial Drugs in Rhodococcosis Treatment

Schlusselhuber¹,

Jung

Bruhn³

et al. 2012

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Email: abrand@ufrgs.br Brandelli, 2008). A recent study indicates that peptide P34 had no adverse effects on eukaryotic cells, similarly to nisin, suggesting its potential as biopreservative (Vaucher, Motta, & Brandelli, 2010). Natamycin is a naturally occurring antifungal agent classified as a macrolide polyene, produced during fermentation by the bacterium Streptomyces natalensis.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of chitosan films containing nisin, peptide P34, and natamycin

Cé

Noreña

Brandelli

2012

CyTA - Journal of Food

Self Cite

View full text Add to dashboard Cite

The antimicrobial effect of edible chitosan films containing nisin, peptide P34, and natamycin was investigated. The activity of chitosan films was tested against Listeria monocytogenes, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Salmonella enteritidis, Clostridium perfringens, Lactobacillus acidophilus, Aspergillus phoenicis, and Penicillium stoloniferum. The addition of nisin and P34 significantly increased the activity against bacteria. Films containing natamycin showed similar inhibition than those with chitosan alone. Chitosan films were effective to control microbial growth in minimally processed pear. Fourier transform infrared spectra of the films were similar, indicating the absence of relevant interaction between the polymer and the antimicrobial agent. The films were also analyzed by scanning electron microscopy to visualize the surface topography. Chitosan films were rough and heterogeneous. The incorporation of antimicrobials agents to edible chitosan films or covering may be a feasible and attractive method for food biopreservation.Keywords: bacteriocin; chitosan; edible film; food protection; polysaccharide El efecto antimicrobiano de pelı´culas comestibles de quitosano formuladas con nisina, pe´ptido P34 y natamicina fue investigado. La actividad de pelı´culas de quitosano se sometio´a prueba con Listeria monocytogenes, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Salmonella enteritidis, Clostridium perfringens, Lactobacillus acidophilus, Aspergillus phoenicis y Penicillium stoloniferum. La adicio´n de nisina y P34 incremento´significativamente la actividad contra las bacterias. Las pelı´culas formuladas con natamicina mostraron una inhibicio´n similar a aquellas con so´lo quitosano. Las pelı´culas de quitosano fueron efectivas para el control del crecimiento microbiano en peras mı´nimamente procesadas. Los espectros FTIR de las pelı´culas fueron similares, indicando la ausencia de interaccio´n relevante entre el polı´mero y el agente antimicrobiano. Las pelı´culas tambie´n se analizaron con microscopı´a electro´nica de barrido para visualizar la topografı´a de la superficie. Las pelı´culas de quitosano fueron a´speras y heteroge´neas. La incorporacio´n de agentes antimicrobianos a pelı´culas o recubrimientos comestibles de quitosano puede ser un me´todo viable y atractivo para la bioconservacio´n de alimentos.

show abstract

“…The antimicrobial peptide P34 was isolated and characterized as a bacteriocin-like substance (BLS) by Motta et al (2007a) and the inhibitory activity was detected against Listeria monocytogenes, Bacillus cereus, and Salmonella Enteritidis (Motta et al 2007b. Furthermore, some studies showed low cytotoxicity in eukaryotic cells and antiviral activity against pathogenic virus of domestic animals (Vaucher et al 2010, Silva et al 2014.…”

mentioning

confidence: 99%

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Costa

Rossatto

Medeiros

et al. 2018

An. Acad. Bras. Ciênc.

Self Cite

View full text Add to dashboard Cite

The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocinlike substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods. The BLS P34 showed inhibitory effect against all planktonic cells of E. faecalis. The inhibition of biofilm formation and the eradication of pre-formed biofilm were evaluated with the crystal violet assay and with the reduction of 3-bromide [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium. The BLS P34 promoted a reduction of percentage of adhered microbial cells on the surface, not being able to perform the complete elimination of biofilm formation. The metabolic activity of S. aureus biofilms decreased considerably between 41-95%. However, E. faecalis cells showed up metabolically stimulated. The BLS P34 has the potential antibiofilm for the species S. aureus. Studies suggest more detailed approaches to a better understanding of the interactions between the antimicrobial and bacterial cells within the biofilm structure.

show abstract

Evaluation of the in vitro cytotoxicity of the antimicrobial peptide P34

Cited by 54 publications

References 42 publications

In Vitro Potential of Equine DEFA1 and eCATH1 as Alternative Antimicrobial Drugs in Rhodococcosis Treatment

In Vitro Potential of Equine DEFA1 and eCATH1 as Alternative Antimicrobial Drugs in Rhodococcosis Treatment

Antimicrobial activity of chitosan films containing nisin, peptide P34, and natamycin

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Contact Info

Product

Resources

About