Evaluation of the Inter- and Intrahospital Spread of Multidrug Resistant Gram-Negative Bacteria in Lithuanian Hospitals

Kirtikliene, Tatjana; Naugžemys, Donatas; Steponkiene, Ana; Bogdevic, Robert; Vizuje, Greta; Žvingila, Donatas; Kuisienė, Nomeda

doi:10.1089/mdr.2018.0160

Cited by 4 publications

(6 citation statements)

References 24 publications

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“…A possible explanation for these results could be the successful genetic adaptation of bacteria over time and the dissemination of resistance genes, which can occur through transmission via medical staff, contaminated equipment, or patients transferring between healthcare institutions [39]. These data are similar to our previously reported results indicating that clinical isolates of E. coli are spread via intra-and/or inter-hospital dissemination between Lithuanian healthcare institutions [10]. Moreover, 17 BOX-PCR profiles each contained two or three isolates.…”

Section: Discussionsupporting

confidence: 89%

“…The most common β-lactamase gene was TEM (98.8% of all isolates). In comparison, in our previous study [10], we observed a lower frequency of this gene (33.9%). CTX-M-9 was the most commonly detected CTX-M group gene (90.6% of all isolates).…”

Section: Discussioncontrasting

confidence: 89%

“…This difference suggests that this gene emerged in Lithuania between 2012 (when the isolates analyzed by Sepp et al were collected) and 2014 (when isolates were collected for the present study). Furthermore, we observed a high frequency of two additional metallo-β-lactamase genes in this study: NDM, which was previously detected in Lithuania [10], and AIM, which was not, were detected in 98.4% and 97.3% of all isolates, respectively.…”

Section: Discussionsupporting

confidence: 62%

“…However, it is less widespread in Lithuania, as the thirdmost common cause of bacterial infection at healthcare institutions. Moreover, in Lithuania the number of multidrug-resistant bloodstream infections caused by E. coli is increasing annually [10]. Therefore, knowledge of virulence factors, antimicrobial resistance determinants, and genotype classifications of bacteremia-causing E. coli strains is important for determining the epidemiological profiles of bloodstream infections, which could be beneficial for establishing specific interventions.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Characterization of Multidrug-Resistant E. coli Isolates from Bloodstream Infections in Lithuania

Kirtikliene

Mierauskaitė²,

Razmienė³

et al. 2022

Microorganisms

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Extraintestinal pathogenic Escherichia coli (ExPEC) isolates are a main cause of bloodstream infections. The aim of this study was to characterize 256 β-lactam–resistant, bacteremia-causing E. coli isolates collected from 12 healthcare institutions in Lithuania in 2014 and 2018. All isolates were identified as E. coli via MALDI-TOF MS and VITEK ®2. In addition, the isolates were analyzed for the presence of 29 resistance genes and 13 virulence genes, divided into phylogenetic groups (A, B1, B2, C, D, E, and F), and characterized using rep-PCR genotyping methods (BOX-PCR and (GTG)5-PCR). Analyzing the results of this study showed tetA-strB-sul2-TEM-NDM-strA-fosA-AIM-sul3-aadA-CTX-M-9 to be the most common resistance gene combination (67.2% of all isolates). Additionally, the most common virulence genes established were fimH (98.4% of all isolates), fyuA (91.8%), and traT (81.3%) and the most common gene combination was fuyA-fimH-iroN (58.6% of all isolates). Next, the isolates were separated into four phylogenetic groups: A, B1, B2, and F, where group A isolates were detected at a significantly higher frequency (79.3% of all isolates). Finally, a total of 235 genotyping profiles were established using rep-PCR methods, and all profiles were separated into fourteen genotypic clusters, with each cluster containing profiles with a variety of virulence and resistance genes not restricted to any specific cluster. The results of this study elucidate E. coli antimicrobial resistance patterns by highlighting the variability and diversity of resistance and virulence genes and providing phylogenetic classification, genetic profiling, and clustering data. These results may improve clinical control of multidrug-resistant infections in healthcare institutions and contribute to the prevention of potential outbreaks.

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Section: Discussionsupporting

confidence: 89%

Section: Discussioncontrasting

confidence: 89%

Section: Discussionsupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

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Genetic Characterization of Multidrug-Resistant E. coli Isolates from Bloodstream Infections in Lithuania

Kirtikliene

Mierauskaitė²,

Razmienė³

et al. 2022

Microorganisms

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“…These data confirmed our previously described results that clinical isolates of Acinetobacter spp. are involved in the intra-and/or inter-hospital dissemination between Lithuanian healthcare institutions [40]. Transmission of endemic clusters of Acinetobacter spp.…”

Section: Discussionmentioning

confidence: 99%

Multidrug-Resistant Acinetobacter baumannii Genetic Characterization and Spread in Lithuania in 2014, 2016, and 2018

Kirtikliene

Mierauskaitė

Razmienė

et al. 2021

Life

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Bacterial resistance to antimicrobial agents plays an important role in the treatment of bacterial infections in healthcare institutions. The spread of multidrug-resistant bacteria can occur during inter- and intra-hospital transmissions among patients and hospital personnel. For this reason, more studies must be conducted to understand how resistance occurs in bacteria and how it moves between hospitals by comparing data from different years and looking out for any patterns that might emerge. Multidrug-resistant (MDR) Acinetobacter spp. was studied at 14 healthcare institutions in Lithuania during 2014, 2016, and 2018 using samples from human bloodstream infections. In total, 194 isolates were collected and identified using MALDI-TOF and VITEK2 analyzers as Acinetobacter baumannii group bacteria. After that, the isolates were analyzed for the presence of different resistance genes (20 genes were analyzed) and characterized by using the Rep-PCR and MLVA (multiple-locus variable-number tandem repeat analysis) genotyping methods. The results of the study showed the relatedness of the different Acinetobacter spp. isolates and a possible circulation of resistance genes or profiles during the different years of the study. This study provides essential information, such as variability and diversity of resistance genes, genetic profiling, and clustering of isolates, to better understand the antimicrobial resistance patterns of Acinetobacter spp. These results can be used to strengthen the control of multidrug-resistant infections in healthcare institutions and to prevent potential outbreaks of this pathogen in the future.

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Emergence of New Delhi Metallo-β-Lactamase (NDM) Genes Detected from Clinical Strains of Escherichia coli Isolated in Ouagadougou, Burkina Faso

Kaboré

Ouédraogo

Oumarou

et al. 2023

International Journal of Microbiology

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The emergence and spread of carbapenem resistance in Gram-negative bacilli such as Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa through the production of carbapenemases is a global phenomenon. It threatens patient care and leads to therapeutic impasses. This study aims to genotypically determine the prevalence of the most frequent carbapenemase genes among multidrug-resistant E. coli strains isolated from patients at a biomedical analysis laboratory. A total of fifty-three unduplicated E. coli strains isolated from patient samples with a multidrug-resistant (MDR) profile were subjected to polymerase chain reaction (PCR) testing for carbapenem resistance genes. This study allowed us to identify fifteen strains carrying resistance genes among the fifty-three E. coli strains. All fifteen strains produced the metallo-β-lactamase enzymes; this represents a rate of 28.30% of study strains. Among these strains, ten carried the NDM resistance gene, NDM and VIM genes were detected in three strains and VIM was identified in two strains of E. coli. However, carbapenemases A (KPC and IMI), D (OXA-48), and IMP were not detected in the strains studied. Thus, NDM and VIM are the main carbapenemases detected in the strains in our study.

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Evaluation of the Inter- and Intrahospital Spread of Multidrug Resistant Gram-Negative Bacteria in Lithuanian Hospitals

Cited by 4 publications

References 24 publications

Genetic Characterization of Multidrug-Resistant E. coli Isolates from Bloodstream Infections in Lithuania

Genetic Characterization of Multidrug-Resistant E. coli Isolates from Bloodstream Infections in Lithuania

Multidrug-Resistant Acinetobacter baumannii Genetic Characterization and Spread in Lithuania in 2014, 2016, and 2018

Emergence of New Delhi Metallo-β-Lactamase (NDM) Genes Detected from Clinical Strains of Escherichia coli Isolated in Ouagadougou, Burkina Faso

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