Evaluation of the radioimmunoassay for benzoylecgonine (a cocaine metabolite) in human urine.

Mulé, S. J.; Jukofsky, D.; Kogan, Michael J.; Pace, A. De; Verebey, Karl

doi:10.1093/clinchem/23.5.796

Cited by 32 publications

(2 citation statements)

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“…The EC and nitrogen detection methods provided a high degree of precision, accuracy, and sensitivity and the FID method was rapid, simple, and also highly accurate. Furthermore, in a comparison between the GLC and Radioimmunoassay (RIA) data reported previously from this laboratory (27), full agreement was shown between the two methods in 95.5% of the 200 urine samples analyzed for benzoylecgonine.…”

Section: Resultsmentioning

confidence: 58%

Quantitative determination of benzoylecgonine and cocaine in human biofluids by gas-liquid chromatography

Kogan¹,

Verebey²,

DePace³

et al. 1977

Anal. Chem.

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Section: Resultsmentioning

confidence: 58%

Quantitative determination of benzoylecgonine and cocaine in human biofluids by gas-liquid chromatography

Kogan¹,

Verebey²,

DePace³

et al. 1977

Anal. Chem.

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“…RIA is a new technique for the determination of ultramicro substances in vitro, which combines the high sensitivity, accuracy, and specificity of radioisotope measurement with the specificity of antigen-antibody reaction. In the initial stage of the development of immunoassays, this method was used for the detection of toxic alkaloids, such as morphine [ 67 ], vinblastine [ 89 ], atropine [ 97 ], solasodine [ 117 ], and cocaine [ 118 ]. Although the method has the advantages of high sensitivity, strong specificity, simple operation, and small sample consumption, the radiation and pollution problems in the analysis process limit the application and development of this method in the detection of toxic alkaloids in plant-derived medicines.…”

Section: Detection Assaymentioning

confidence: 99%

Progress in Immunoassays of Toxic Alkaloids in Plant-Derived Medicines: A Review

Ren

Zhang

Wang

et al. 2022

Toxins

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Plants are the cradle of the traditional medicine system, assuaging human or animal diseases, and promoting health for thousands of years. However, many plant-derived medicines contain toxic alkaloids of varying degrees of toxicity that pose a direct or indirect threat to human and animal health through accidental ingestion, misuse of plant materials, or through the food chain. Thus, rapid, easy, and sensitive methods are needed to effectively screen these toxic alkaloids to guarantee the safety of plant-derived medicines. Antibodies, due to their inherent specificity and high affinity, have been used as a variety of analytical tools and techniques. This review describes the antigen synthesis and antibody preparation of the common toxic alkaloids in plant-derived medicines and discusses the advances of antibody-based immunoassays in the screening and detection of toxic alkaloids in plants or other related matrices. Finally, the limitations and prospects of immunoassays for toxic alkaloids are discussed.

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Immunoassays in Forensic Toxicology

Moody¹

2000

Encyclopedia of Analytical Chemistry

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Forensic toxicology encompasses the determination of the presence and concentration of drugs, other xenobiotics and their metabolites in physiological fluids and organs and the interpretation of these findings as they may impact on legal issues. These include medical examiner investigations, driving under the influence and other transportation accident investigations, workplace pre‐employment, random and for‐cause drug testing and judicial monitoring of arrestees and parolees. Similar techniques are employed in emergency room clinical toxicology and to monitor the efficacy of substance abuse treatment. The introduction of immunoassays into forensic toxicology in the early 1970s has had a major impact on the speed and efficiency that samples can be screened for the presence of certain drug classes. For the most part, forensic toxicologists use commercial immunoassays directed primarily towards abused drugs. Commercial immunoassays developed for therapeutic monitoring of other drugs, veterinary drugs and pesticides, as well as immunoassays developed in research laboratories for specialized studies, may find a role in the forensic toxicology laboratory for specialized cases. Most immunoassays used for forensic toxicology are competitive. An antigen structurally similar to the target compound is conjugated to a signalling molecule and competes with target drug in a sample for antibody binding. Immunoassays are also classified as homogeneous and heterogeneous. Homogeneous assays do not separate the original sample from the final detection sample. They must use a signal that changes when antibody is bound. Homogeneous immunoassays include enzyme immunoassay (EIA) (enzyme activity decreases when bound), fluorescent polarization immunoassay (FPIA) (emission in a polar field increases when bound) and kinetic interaction of microparticles in solution (KIMS) immunoassay (lattice formation inhibited when bound). Heterogenous immunoassays include radioimmunoassay (RIA) and enzyme‐linked immunosorbent assay (ELISA) where unbound radiolabeled antigen and enzyme conjugated antigen, respectively, are removed from the sample before measurement. In general, the homogeneous immunoassays are more ameniable to full automation, and thereby quicker throughput. The heterogeneous immunoassays are less susceptible to matrix interference, and thereby more versatile with nonurine matrices. While most commercial immunoassays have been developed for a urine matrix, they have been applied by forensic toxicologists to other matrices, including blood, hair, saliva, sweat, tissue homogenates, blood stains and most other physiological samples that may be of value in the investigation. The use of nonurine matrices must contend with two factors. With the exception of parenchymal tissues, the concentration of the target compound is often lower and the sensitivity of the immunoassay may be limiting. In addition, the nonurine matrix usually is much more complex in its composition. Sample pretreatments that range from simple deproteinations to multistep extractions to remove matrix components and/or concentrate the sample are often required. The heterogenous RIAs and ELISAs usually require less rigorous, if any, pretreatments. Interpretation of immunoassay results must take into consideration the limits of detection of the assay, the cross‐reactivity of the antibody(ies) and the potential for interference. Appropriate controls should be included to demonstrate adequate signal separation from blanks (drug‐free sample in the same matrix). The concentration of the low control is often determined by the sensitivity of the assay, or in workplace testing programs by administratively determined cutoffs that draw the line between a negative and presumptive positive. The ability of the antibody to detect compounds other than the target compound (its cross‐reactivity) can be a useful characteristic or, when the drugs that can be confirmed are limited, a nuisance. The amphetamines, barbiturates and benzodiazepines, in particular, all have a number of licit (and for amphetamines illicit) analogs that could be present in a sample. Cross‐reactivities are antibody‐source (i.e. manufacturer) dependent. Further testing to determine the immunoreactive compound often requires rigorous methodologies. In some instances, the potency of the drug and its poor cross‐reactivity make detection by immunoassay difficult (e.g. nitrosobenzodiazepines). Interference in immunoassays may arise from compounds that appear in the matrix during disease states, or those that are intentionally added to a sample in the hope of negating a positive test. These act through many different mechanisms and may decrease or increase the immunoassay test result. Immunoassays have added an extremely useful tool to the forensic toxicology investigation. They can be used to screen rapidly a large number of samples for the potential presence of a drug group. With rare exceptions (emergencies, limited sample volume), their use without a confirmation assay (e.g. gas or liquid chromatography/mass spectrometry (LC/MS)) is unwarranted, as it leads to a risk of improper test result interpretation.

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Evaluation of the radioimmunoassay for benzoylecgonine (a cocaine metabolite) in human urine.

Cited by 32 publications

References 0 publications

Quantitative determination of benzoylecgonine and cocaine in human biofluids by gas-liquid chromatography

Quantitative determination of benzoylecgonine and cocaine in human biofluids by gas-liquid chromatography

Progress in Immunoassays of Toxic Alkaloids in Plant-Derived Medicines: A Review

Immunoassays in Forensic Toxicology

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