Evaluation of the Thermal Stability of a Vaccine Prototype Based on Virus-like Particle Formulated HIV-1 Envelope

Aguado-Garcia, Diana; Olvera, Àlex; Berthou, Christian; Sánchez-Merino, Víctor; Yuste, Eloísa

doi:10.3390/vaccines10040484

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…Comparative Analysis. Compared to freeze-drying and genetic engineering to improve the thermal stability of influenza vaccine, 27 human immunodeficiency virus type 1 vaccine, 60 and classical swine fever vaccine, 61 HSZ does not require a complex preparation process, can be performed in a short time, and is cost-effective (Table S2). Long-term stability is also a key factor in the use of vaccines, and this part of the study is the focus of our subsequent research.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Silk Fibroin-Coated Nano-MOFs Enhance the Thermal Stability and Immunogenicity of HBsAg

Zhang,

Wang,

et al. 2024

ACS Appl. Mater. Interfaces

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Vaccines are widely regarded as one of the most effective weapons in the fight against infectious diseases. Currently, vaccines must be stored and transported at low temperatures as high temperatures can lead to a loss of vaccine conformation and reduced therapeutic efficacy. Metal−organic frameworks (MOFs), such as zeolitic imidazole framework-8 (ZIF-8), are a new class of hybrid materials with large specific surface areas, high loading rates, and good biocompatibility and are successful systems for vaccine delivery and protection. Silk fibroin (SF) has a good biocompatibility and thermal stability. In this study, the hepatitis B surface antigen (HBsAg) was successfully encapsulated in ZIF-8 to form HBsAg@ZIF-8 (HZ) using a one-step shake and one-pot shake method. Subsequently, the SF coating modifies HZ through hydrophobic interactions to form HBsAg/SF@ZIF-8 (HSZ), which enhanced the thermal stability and immunogenicity of HBsAg. Compared to free HBsAg, HZ and HSZ improved the thermostability of HBsAg, promoted the antigen uptake and lysosomal escape, stimulated dendritic cell maturation and cytokine secretion, formed an antigen reservoir to promote antibody production, and activated CD4 + T and CD8 + T cells to enhance memory T-cell production. Importantly, HSZ induced a strong immune response even after 14 days of storage at 25 °C. Furthermore, the nanoparticles prepared by the one-step shake method exhibited superior properties compared to those prepared by the one-pot shake method. This study highlights the importance of SF-coated ZIF-8, which holds promise for investigating thermostable vaccines and breaking the vaccine cold chain.

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Section: ■ Experimental Sectionmentioning

confidence: 99%

Silk Fibroin-Coated Nano-MOFs Enhance the Thermal Stability and Immunogenicity of HBsAg

Zhang,

Wang,

et al. 2024

ACS Appl. Mater. Interfaces

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“…With the accelerated advancement of nanotechnology and protein engineering, interesting capabilities have been developed for the development and improvement of vaccines and epitope presentation or drug delivery systems (DDSs). Therefore, one possible design inserting carbonbased nanomaterials (CBNs) to induce the synthesis of speci c metabolic pathways, has become an attractive alternative for many researchers in the design and development of novel nanovaccines [28,31,32].…”

Section: What Are the Technological Strategies Advances And Challenge...mentioning

confidence: 99%

Carbon Nanomaterials: Revolutionary Catalysts for the Development of Vaccines with Virus-like Particles

Lima,

Justo,

Piqueira

2023

Preprint

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Virus-like particle (VLP) vaccines are powerful immunostimulants that efficiently trigger robust B-cell and T-cell responses, specifically enhancing the activity of dendritic cells. The integration of nanoparticles, particularly carbon-based nanomaterials (CBNs), with these VLPs, has opened new horizons in creating targeted, durable, and potent immune responses. This innovative approach may be pivotal in formulating therapeutic vaccines against notoriously challenging pathogens, including HIV. Functionalized CBNs have shown the potential to amplify cellular uptake, fostering a highly specific immune activation. The employment of VLP vaccines in conjunction with CBNs has sparked intensive research efforts, with a major focus on nanostructure functionalization for augmenting immune response specificity and strength. In this meta-review, we synthesize, critique, and discuss the current landscape of VLP-based vaccines and CBNs as a dynamic vaccine platform. The goal is to strategize the prevention and treatment of various viral infections, with a special focus on coronaviruses and HIV, paving the way for next-generation antiviral therapeutics.

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Surface functionalization of virus-like particles via bioorthogonal click reactions for enhanced cell-specific targeting

Laomeephol,

Tawinwung,

Suppipat

et al. 2024

International Journal of Pharmaceutics

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Evaluation of the Thermal Stability of a Vaccine Prototype Based on Virus-like Particle Formulated HIV-1 Envelope

Cited by 4 publications

References 14 publications

Silk Fibroin-Coated Nano-MOFs Enhance the Thermal Stability and Immunogenicity of HBsAg

Silk Fibroin-Coated Nano-MOFs Enhance the Thermal Stability and Immunogenicity of HBsAg

Carbon Nanomaterials: Revolutionary Catalysts for the Development of Vaccines with Virus-like Particles

Surface functionalization of virus-like particles via bioorthogonal click reactions for enhanced cell-specific targeting

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